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Dominance of Haemophilus influenzae in ear discharge from Indigenous Australian children with acute otitis media with tympanic membrane perforation.

Smith-Vaughan HC, Binks MJ, Marsh RL, Kaestli M, Ward L, Hare KM, Pizzutto SJ, Thornton RB, Morris PS, Leach AJ - BMC Ear Nose Throat Disord (2013)

Bottom Line: S. pneumoniae and M. catarrhalis abundances were significantly lower in ear discharge compared with nasopharyngeal swabs (p = 0.001, p < 0.001); no significant difference was observed in H. influenzae mean abundance at the two sites.High prevalence and abundance of S. pneumoniae and M. catarrhalis in the nasopharynx did not predict ear discharge prevalence and abundances of these pathogens.Quantitative methods are required to understand species abundance in polymicrobial infections and may be needed to measure accurately the microbiological impact of interventions and to provide a better understanding of clinical failure in these children.

View Article: PubMed Central - HTML - PubMed

Affiliation: Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia. heidi@menzies.edu.au.

ABSTRACT

Background: Indigenous Australian children living in remote communities experience high rates of acute otitis media with tympanic membrane perforation (AOMwiP). Otitis media in this population is associated with dense nasopharyngeal colonization of three primary otopathogens; Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis. Little is known about the relative abundance of these pathogens during infection. The objective of this study was to estimate the abundance and concordance of otopathogens in ear discharge and paired nasopharyngeal swabs from children with AOMwiP (discharge of not more than 6 weeks' duration and perforation size <2%).

Methods: Culture and quantitative PCR (qPCR) estimation of H. influenzae, S. pneumoniae, M. catarrhalis and total bacterial load were performed on paired nasopharyngeal and ear discharge swabs from 55 Indigenous children with AOMwiP aged 3.5 - 45.6 months and resident in remote communities.

Results: By culture, H. influenzae, S. pneumoniae, and M. catarrhalis were detected in 80%, 84% and 91% of nasopharyngeal swabs, and 49%, 33% and 4% of ear discharge swabs, respectively. Using qPCR, H. influenzae, S. pneumoniae, and M. catarrhalis were detected in 82%, 82%, and 93% of nasopharyngeal swabs, and 89%, 41% and 18% of ear discharge swabs, respectively. Relative abundance of H. influenzae in ear discharge swabs was 0-68% of the total bacterial load (median 2.8%); whereas S. pneumoniae and M. catarrhalis relative abundances were consistently <2% of the total bacterial load. S. pneumoniae and M. catarrhalis abundances were significantly lower in ear discharge compared with nasopharyngeal swabs (p = 0.001, p < 0.001); no significant difference was observed in H. influenzae mean abundance at the two sites.

Conclusions: H. influenzae was the dominant otopathogen detected in ear discharge swabs collected from children with AOMwiP. High prevalence and abundance of S. pneumoniae and M. catarrhalis in the nasopharynx did not predict ear discharge prevalence and abundances of these pathogens. PCR was substantially more sensitive than culture for ear discharge, and a necessary adjunct to standard microbiology. Quantitative methods are required to understand species abundance in polymicrobial infections and may be needed to measure accurately the microbiological impact of interventions and to provide a better understanding of clinical failure in these children.

No MeSH data available.


Related in: MedlinePlus

Relative abundance of H. influenzae, S. pneumoniae and M. catarrhalis in nasopharyngeal and ear discharge swabs. Median relative abundance and interquartile ranges for H. influenzae, S. pneumoniae, M. catarrhalis and a combination of the three bacteria as a proportion of total bacterial load in nasopharyngeal (a) and ear discharge (b) swabs.
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Figure 4: Relative abundance of H. influenzae, S. pneumoniae and M. catarrhalis in nasopharyngeal and ear discharge swabs. Median relative abundance and interquartile ranges for H. influenzae, S. pneumoniae, M. catarrhalis and a combination of the three bacteria as a proportion of total bacterial load in nasopharyngeal (a) and ear discharge (b) swabs.

Mentions: The relative abundances of individual species were estimated as a proportion of the total bacterial load. The median relative abundances of H. influenzae, S. pneumoniae and M. catarrhalis in nasopharyngeal swabs was 3.8% (range 0-48%), 2% (0-43%) and 13% (0-44%), respectively. In contrast, the median relative abundance of S. pneumoniae (0%; range, 0-12%) and M. catarrhalis (0%, range 0–1.8%) in ear discharge was low. The median H. influenzae abundance in ear discharge was marginally higher at 2.8%, but with a range of up to 68% of the total bacterial load. Figure 4 illustrates the median proportions of H. influenzae, S. pneumoniae and M. catarrhalis in nasopharyngeal and ear discharge swabs demonstrating the dominance of H. influenzae in ear discharge. Notably, a large proportion of the total bacterial load represented bacteria that we did not identify, and which may have originated from the middle ear or the ear canal.


Dominance of Haemophilus influenzae in ear discharge from Indigenous Australian children with acute otitis media with tympanic membrane perforation.

Smith-Vaughan HC, Binks MJ, Marsh RL, Kaestli M, Ward L, Hare KM, Pizzutto SJ, Thornton RB, Morris PS, Leach AJ - BMC Ear Nose Throat Disord (2013)

Relative abundance of H. influenzae, S. pneumoniae and M. catarrhalis in nasopharyngeal and ear discharge swabs. Median relative abundance and interquartile ranges for H. influenzae, S. pneumoniae, M. catarrhalis and a combination of the three bacteria as a proportion of total bacterial load in nasopharyngeal (a) and ear discharge (b) swabs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3852835&req=5

Figure 4: Relative abundance of H. influenzae, S. pneumoniae and M. catarrhalis in nasopharyngeal and ear discharge swabs. Median relative abundance and interquartile ranges for H. influenzae, S. pneumoniae, M. catarrhalis and a combination of the three bacteria as a proportion of total bacterial load in nasopharyngeal (a) and ear discharge (b) swabs.
Mentions: The relative abundances of individual species were estimated as a proportion of the total bacterial load. The median relative abundances of H. influenzae, S. pneumoniae and M. catarrhalis in nasopharyngeal swabs was 3.8% (range 0-48%), 2% (0-43%) and 13% (0-44%), respectively. In contrast, the median relative abundance of S. pneumoniae (0%; range, 0-12%) and M. catarrhalis (0%, range 0–1.8%) in ear discharge was low. The median H. influenzae abundance in ear discharge was marginally higher at 2.8%, but with a range of up to 68% of the total bacterial load. Figure 4 illustrates the median proportions of H. influenzae, S. pneumoniae and M. catarrhalis in nasopharyngeal and ear discharge swabs demonstrating the dominance of H. influenzae in ear discharge. Notably, a large proportion of the total bacterial load represented bacteria that we did not identify, and which may have originated from the middle ear or the ear canal.

Bottom Line: S. pneumoniae and M. catarrhalis abundances were significantly lower in ear discharge compared with nasopharyngeal swabs (p = 0.001, p < 0.001); no significant difference was observed in H. influenzae mean abundance at the two sites.High prevalence and abundance of S. pneumoniae and M. catarrhalis in the nasopharynx did not predict ear discharge prevalence and abundances of these pathogens.Quantitative methods are required to understand species abundance in polymicrobial infections and may be needed to measure accurately the microbiological impact of interventions and to provide a better understanding of clinical failure in these children.

View Article: PubMed Central - HTML - PubMed

Affiliation: Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia. heidi@menzies.edu.au.

ABSTRACT

Background: Indigenous Australian children living in remote communities experience high rates of acute otitis media with tympanic membrane perforation (AOMwiP). Otitis media in this population is associated with dense nasopharyngeal colonization of three primary otopathogens; Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis. Little is known about the relative abundance of these pathogens during infection. The objective of this study was to estimate the abundance and concordance of otopathogens in ear discharge and paired nasopharyngeal swabs from children with AOMwiP (discharge of not more than 6 weeks' duration and perforation size <2%).

Methods: Culture and quantitative PCR (qPCR) estimation of H. influenzae, S. pneumoniae, M. catarrhalis and total bacterial load were performed on paired nasopharyngeal and ear discharge swabs from 55 Indigenous children with AOMwiP aged 3.5 - 45.6 months and resident in remote communities.

Results: By culture, H. influenzae, S. pneumoniae, and M. catarrhalis were detected in 80%, 84% and 91% of nasopharyngeal swabs, and 49%, 33% and 4% of ear discharge swabs, respectively. Using qPCR, H. influenzae, S. pneumoniae, and M. catarrhalis were detected in 82%, 82%, and 93% of nasopharyngeal swabs, and 89%, 41% and 18% of ear discharge swabs, respectively. Relative abundance of H. influenzae in ear discharge swabs was 0-68% of the total bacterial load (median 2.8%); whereas S. pneumoniae and M. catarrhalis relative abundances were consistently <2% of the total bacterial load. S. pneumoniae and M. catarrhalis abundances were significantly lower in ear discharge compared with nasopharyngeal swabs (p = 0.001, p < 0.001); no significant difference was observed in H. influenzae mean abundance at the two sites.

Conclusions: H. influenzae was the dominant otopathogen detected in ear discharge swabs collected from children with AOMwiP. High prevalence and abundance of S. pneumoniae and M. catarrhalis in the nasopharynx did not predict ear discharge prevalence and abundances of these pathogens. PCR was substantially more sensitive than culture for ear discharge, and a necessary adjunct to standard microbiology. Quantitative methods are required to understand species abundance in polymicrobial infections and may be needed to measure accurately the microbiological impact of interventions and to provide a better understanding of clinical failure in these children.

No MeSH data available.


Related in: MedlinePlus