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Concordance between in vivo and postmortem measurements of cholinergic denervation in rats using PET with [18F]FEOBV and choline acetyltransferase immunochemistry.

Parent MJ, Cyr M, Aliaga A, Kostikov A, Schirrmacher E, Soucy JP, Mechawar N, Rosa-Neto P, Bedard MA - EJNMMI Res (2013)

Bottom Line: For both PET binding and postmortem OD, the highest losses were found in the cortical areas, with the highest reductions in the orbitofrontal, sensorimotor, and cingulate cortices.In addition, OD quantification in the affected areas accurately predicts [18F]FEOBV uptake in the same regions when regressed linearly.These findings support [18F]FEOBV as a reliable imaging agent for eventual use in human neurodegenerative conditions in which cholinergic losses are an important aspect.

View Article: PubMed Central - HTML - PubMed

Affiliation: Douglas Mental Health University Institute, McGill University, Montreal, QC H4H 1R3, Canada. maxime.parent@mail.mcgill.ca.

ABSTRACT

Background: Fluorine-18 fluoroethoxybenzovesamicol ([18F]FEOBV) is a radioligand for the selective imaging of the vesicular acetylcholine transporter with positron emission tomography (PET). The current study demonstrates that pathological cortical cholinergic deafferentation can be quantified in vivo with [18F]FEOBV PET, yielding analogous results to postmortem histological techniques.

Methods: Fifteen male rats (3 months old) underwent a cerebral infusion of 192 IgG-saporin at the level of the nucleus basalis magnocellularis. They were scanned using [18F]FEOBV PET, then sacrificed, and their brain tissues collected for immunostaining and quantification of cholinergic denervation using optical density (OD).

Results: For both PET binding and postmortem OD, the highest losses were found in the cortical areas, with the highest reductions in the orbitofrontal, sensorimotor, and cingulate cortices. In addition, OD quantification in the affected areas accurately predicts [18F]FEOBV uptake in the same regions when regressed linearly.

Conclusions: These findings support [18F]FEOBV as a reliable imaging agent for eventual use in human neurodegenerative conditions in which cholinergic losses are an important aspect.

No MeSH data available.


Related in: MedlinePlus

[18F]FEOBV correlation maps. Left cortex optical density of ChAT-immunostained slices correlates with [18F]FEOBV DVR in a cluster located in the left frontal cortex. Right cortex optical density correlates with a cluster in the right frontal cortex.
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Figure 4: [18F]FEOBV correlation maps. Left cortex optical density of ChAT-immunostained slices correlates with [18F]FEOBV DVR in a cluster located in the left frontal cortex. Right cortex optical density correlates with a cluster in the right frontal cortex.

Mentions: Regression analysis between the [18F]FEOBV DVR at the voxel level (see Figure 4) and the OD values for the whole cortex of the left hemisphere reveals a correlation cluster in the dorsal area of the left frontal cortex (26.35 mm3, peak r(12) = 81%, p = 0.0004). Conversely, OD values of the whole right hemisphere correlate significantly with a symmetrical (although smaller) cluster of [18F]FEOBV DVR in the right frontal cortex (17.28mm3, peak r(12) = 73%, p = 0.003). When using only OD values restricted to the same cortical area as the [18F]FEOBV DVR cluster in the dorsal left frontal cortex (see Figure 5), a significant correlation of 75% (p = 0.002) was still present.


Concordance between in vivo and postmortem measurements of cholinergic denervation in rats using PET with [18F]FEOBV and choline acetyltransferase immunochemistry.

Parent MJ, Cyr M, Aliaga A, Kostikov A, Schirrmacher E, Soucy JP, Mechawar N, Rosa-Neto P, Bedard MA - EJNMMI Res (2013)

[18F]FEOBV correlation maps. Left cortex optical density of ChAT-immunostained slices correlates with [18F]FEOBV DVR in a cluster located in the left frontal cortex. Right cortex optical density correlates with a cluster in the right frontal cortex.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3852759&req=5

Figure 4: [18F]FEOBV correlation maps. Left cortex optical density of ChAT-immunostained slices correlates with [18F]FEOBV DVR in a cluster located in the left frontal cortex. Right cortex optical density correlates with a cluster in the right frontal cortex.
Mentions: Regression analysis between the [18F]FEOBV DVR at the voxel level (see Figure 4) and the OD values for the whole cortex of the left hemisphere reveals a correlation cluster in the dorsal area of the left frontal cortex (26.35 mm3, peak r(12) = 81%, p = 0.0004). Conversely, OD values of the whole right hemisphere correlate significantly with a symmetrical (although smaller) cluster of [18F]FEOBV DVR in the right frontal cortex (17.28mm3, peak r(12) = 73%, p = 0.003). When using only OD values restricted to the same cortical area as the [18F]FEOBV DVR cluster in the dorsal left frontal cortex (see Figure 5), a significant correlation of 75% (p = 0.002) was still present.

Bottom Line: For both PET binding and postmortem OD, the highest losses were found in the cortical areas, with the highest reductions in the orbitofrontal, sensorimotor, and cingulate cortices.In addition, OD quantification in the affected areas accurately predicts [18F]FEOBV uptake in the same regions when regressed linearly.These findings support [18F]FEOBV as a reliable imaging agent for eventual use in human neurodegenerative conditions in which cholinergic losses are an important aspect.

View Article: PubMed Central - HTML - PubMed

Affiliation: Douglas Mental Health University Institute, McGill University, Montreal, QC H4H 1R3, Canada. maxime.parent@mail.mcgill.ca.

ABSTRACT

Background: Fluorine-18 fluoroethoxybenzovesamicol ([18F]FEOBV) is a radioligand for the selective imaging of the vesicular acetylcholine transporter with positron emission tomography (PET). The current study demonstrates that pathological cortical cholinergic deafferentation can be quantified in vivo with [18F]FEOBV PET, yielding analogous results to postmortem histological techniques.

Methods: Fifteen male rats (3 months old) underwent a cerebral infusion of 192 IgG-saporin at the level of the nucleus basalis magnocellularis. They were scanned using [18F]FEOBV PET, then sacrificed, and their brain tissues collected for immunostaining and quantification of cholinergic denervation using optical density (OD).

Results: For both PET binding and postmortem OD, the highest losses were found in the cortical areas, with the highest reductions in the orbitofrontal, sensorimotor, and cingulate cortices. In addition, OD quantification in the affected areas accurately predicts [18F]FEOBV uptake in the same regions when regressed linearly.

Conclusions: These findings support [18F]FEOBV as a reliable imaging agent for eventual use in human neurodegenerative conditions in which cholinergic losses are an important aspect.

No MeSH data available.


Related in: MedlinePlus