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Metabolic markers and microecological characteristics of tongue coating in patients with chronic gastritis.

Sun ZM, Zhao J, Qian P, Wang YQ, Zhang WF, Guo CR, Pang XY, Wang SC, Li FF, Li Q - BMC Complement Altern Med (2013)

Bottom Line: Strip 10 sequencing showed a 100% similarity to Rothia mucilaginosa.Strip 8 sequencing showed a 96.2% similarity to Moraxella catarrhalis.Compared with other diagnostic regimens, such as blood tests or tissue biopsies, tongue coating is more amenable to, and more convenient for, both patients and doctors.

View Article: PubMed Central - HTML - PubMed

Affiliation: Shanghai University of Traditional Chinese Medicine, Cai Lun Road, Pu dong district, Shanghai 201203, China. li_fufeng@aliyun.com.

ABSTRACT

Background: In Traditional Chinese Medicine (TCM), tongue diagnosis has been an important diagnostic method for the last 3000 years. Tongue diagnosis is a non-invasive, simple and valuable diagnostic tool. TCM treats the tongue coating on a very sensitive scale that reflects physiological and pathological changes in the organs, especially the spleen and stomach. Tongue coating can diagnose disease severity and determine the TCM syndrome ("Zheng" in Chinese). The biological bases of different tongue coating appearances are still poorly understood and lack systematic investigation at the molecular level.

Methods: Tongue coating samples were collected from 70 chronic gastritis patients and 20 normal controls. 16S rRNA denatured gradient gel electrophoresis (16S rRNA-DGGE) and liquid chromatography and mass spectrometry (LC-MS) were designed to profile tongue coatings. The statistical techniques used were principal component analysis and partial least squares-discriminate analysis.

Results: Ten potential metabolites or markers were found in chronic gastritis patients, including UDP-D-galactose, 3-ketolactose, and vitamin D2, based on LC-MS. Eight significantly different strips were observed in samples from chronic gastritis patients based on 16S rRNA-DGGE. Two strips, Strips 8 and 10, were selected for gene sequencing. Strip 10 sequencing showed a 100% similarity to Rothia mucilaginosa. Strip 8 sequencing showed a 96.2% similarity to Moraxella catarrhalis.

Conclusions: Changes in glucose metabolism could possibly form the basis of tongue coating conformation in chronic gastritis patients. The study revealed important connections between metabolic components, microecological components and tongue coating in chronic gastritis patients. Compared with other diagnostic regimens, such as blood tests or tissue biopsies, tongue coating is more amenable to, and more convenient for, both patients and doctors.

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PCR–DGGE atlas of 16S rRNA gene V3 region between the chronic gastritis group and the normal controls. (A) Normal group, M1-marker, M2- control,2,34,6,7,8,9,10,11,12,13,15,17,18,19- normal groupsample. (B) Chronic gastritis group, M1-marker, M2-control,2,34,6,7,8,9,10,11,12,13,15,17,18,19- sample.
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Figure 5: PCR–DGGE atlas of 16S rRNA gene V3 region between the chronic gastritis group and the normal controls. (A) Normal group, M1-marker, M2- control,2,34,6,7,8,9,10,11,12,13,15,17,18,19- normal groupsample. (B) Chronic gastritis group, M1-marker, M2-control,2,34,6,7,8,9,10,11,12,13,15,17,18,19- sample.

Mentions: PCR products of the bacterial 16S rRNA gene V3 region from two groups of tongue coating samples were processed using DGGE. The atlas is shown in Figure 5. Examination of the DGGE atlas revealed numerous bacterial DGGE atlas strips and some main strips in the two groups.


Metabolic markers and microecological characteristics of tongue coating in patients with chronic gastritis.

Sun ZM, Zhao J, Qian P, Wang YQ, Zhang WF, Guo CR, Pang XY, Wang SC, Li FF, Li Q - BMC Complement Altern Med (2013)

PCR–DGGE atlas of 16S rRNA gene V3 region between the chronic gastritis group and the normal controls. (A) Normal group, M1-marker, M2- control,2,34,6,7,8,9,10,11,12,13,15,17,18,19- normal groupsample. (B) Chronic gastritis group, M1-marker, M2-control,2,34,6,7,8,9,10,11,12,13,15,17,18,19- sample.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3852724&req=5

Figure 5: PCR–DGGE atlas of 16S rRNA gene V3 region between the chronic gastritis group and the normal controls. (A) Normal group, M1-marker, M2- control,2,34,6,7,8,9,10,11,12,13,15,17,18,19- normal groupsample. (B) Chronic gastritis group, M1-marker, M2-control,2,34,6,7,8,9,10,11,12,13,15,17,18,19- sample.
Mentions: PCR products of the bacterial 16S rRNA gene V3 region from two groups of tongue coating samples were processed using DGGE. The atlas is shown in Figure 5. Examination of the DGGE atlas revealed numerous bacterial DGGE atlas strips and some main strips in the two groups.

Bottom Line: Strip 10 sequencing showed a 100% similarity to Rothia mucilaginosa.Strip 8 sequencing showed a 96.2% similarity to Moraxella catarrhalis.Compared with other diagnostic regimens, such as blood tests or tissue biopsies, tongue coating is more amenable to, and more convenient for, both patients and doctors.

View Article: PubMed Central - HTML - PubMed

Affiliation: Shanghai University of Traditional Chinese Medicine, Cai Lun Road, Pu dong district, Shanghai 201203, China. li_fufeng@aliyun.com.

ABSTRACT

Background: In Traditional Chinese Medicine (TCM), tongue diagnosis has been an important diagnostic method for the last 3000 years. Tongue diagnosis is a non-invasive, simple and valuable diagnostic tool. TCM treats the tongue coating on a very sensitive scale that reflects physiological and pathological changes in the organs, especially the spleen and stomach. Tongue coating can diagnose disease severity and determine the TCM syndrome ("Zheng" in Chinese). The biological bases of different tongue coating appearances are still poorly understood and lack systematic investigation at the molecular level.

Methods: Tongue coating samples were collected from 70 chronic gastritis patients and 20 normal controls. 16S rRNA denatured gradient gel electrophoresis (16S rRNA-DGGE) and liquid chromatography and mass spectrometry (LC-MS) were designed to profile tongue coatings. The statistical techniques used were principal component analysis and partial least squares-discriminate analysis.

Results: Ten potential metabolites or markers were found in chronic gastritis patients, including UDP-D-galactose, 3-ketolactose, and vitamin D2, based on LC-MS. Eight significantly different strips were observed in samples from chronic gastritis patients based on 16S rRNA-DGGE. Two strips, Strips 8 and 10, were selected for gene sequencing. Strip 10 sequencing showed a 100% similarity to Rothia mucilaginosa. Strip 8 sequencing showed a 96.2% similarity to Moraxella catarrhalis.

Conclusions: Changes in glucose metabolism could possibly form the basis of tongue coating conformation in chronic gastritis patients. The study revealed important connections between metabolic components, microecological components and tongue coating in chronic gastritis patients. Compared with other diagnostic regimens, such as blood tests or tissue biopsies, tongue coating is more amenable to, and more convenient for, both patients and doctors.

Show MeSH
Related in: MedlinePlus