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The cytology, isozyme, HPLC fingerprint, and interspecific hybridization studies of genus epimedium (berberidaceae).

Wang LJ, Sheng MY - ISRN Pharmacol (2013)

Bottom Line: Chromosomes C-banding of barrenwort species varies, with 15 to 22 bands, consisting of centromeric bands, intercalary bands, terminal bands, and middle satellite bands.Results of POD isozyme showed that the zymographs vary greatly and sixteen bands were detected in the eleven species, and each species has its own characteristic bands different from the others.Based on these results, the system and phylogeny of this genus were inferred.

View Article: PubMed Central - PubMed

Affiliation: School of Vocation and Technology, Guizhou Normal University, Guiyang, Guizhou 550001, China.

ABSTRACT
104 samples from 27 accessions belonging to 12 species of genus Epimedium were studied on the basis of cytology observation, POD (i.e., peroxide) isozyme, high performance liquid chromatography (i.e., HPLC) fingerprint, and interspecific hybridization. The cytology observation showed karyotypes of twelve species studied; all are 2A symmetry type of Stebbins standard and similar to each other, and except for karyotype of E. leptorrhizum which is 2n = 2x = 8m (2SAT) + 4sm, the rest are 2n = 2x = 6m (2SAT) + 6sm. Chromosomes C-banding of barrenwort species varies, with 15 to 22 bands, consisting of centromeric bands, intercalary bands, terminal bands, and middle satellite bands. Results of POD isozyme showed that the zymographs vary greatly and sixteen bands were detected in the eleven species, and each species has its own characteristic bands different from the others. Studies on the HPLC fingerprint showed that the HPLC fingerprint of different species has characteristic peaks, divided into two regions (retention time < 10 min and retention time > 10 min). Results of interspecific hybridization showed that crosses of any combination among seven species studied are successful and the rates of grain set vary greatly. Based on these results, the system and phylogeny of this genus were inferred.

No MeSH data available.


Related in: MedlinePlus

Clustering of seven species by the grain set rate of hybridization.
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fig8: Clustering of seven species by the grain set rate of hybridization.

Mentions: Results also showed that there are no various differences between the grain set rates of positive and negative hybridization, suggesting that the significance of grain set rats of positive and negative hybridization in symboling the interspecific relationship of this genus is the same. So the means of grain set rats of positive and negative hybridization were analyzed by the unweighted pair-group method (UPGMA) (Figure 8) to find the relationship of seven species researched in this paper. Results showed that E. myrianthum is very distant from other six species, and the relation between E. luodianense and E. yinjiangense is the nearest, and they are nearer related to E. letorrhizum.


The cytology, isozyme, HPLC fingerprint, and interspecific hybridization studies of genus epimedium (berberidaceae).

Wang LJ, Sheng MY - ISRN Pharmacol (2013)

Clustering of seven species by the grain set rate of hybridization.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3852721&req=5

fig8: Clustering of seven species by the grain set rate of hybridization.
Mentions: Results also showed that there are no various differences between the grain set rates of positive and negative hybridization, suggesting that the significance of grain set rats of positive and negative hybridization in symboling the interspecific relationship of this genus is the same. So the means of grain set rats of positive and negative hybridization were analyzed by the unweighted pair-group method (UPGMA) (Figure 8) to find the relationship of seven species researched in this paper. Results showed that E. myrianthum is very distant from other six species, and the relation between E. luodianense and E. yinjiangense is the nearest, and they are nearer related to E. letorrhizum.

Bottom Line: Chromosomes C-banding of barrenwort species varies, with 15 to 22 bands, consisting of centromeric bands, intercalary bands, terminal bands, and middle satellite bands.Results of POD isozyme showed that the zymographs vary greatly and sixteen bands were detected in the eleven species, and each species has its own characteristic bands different from the others.Based on these results, the system and phylogeny of this genus were inferred.

View Article: PubMed Central - PubMed

Affiliation: School of Vocation and Technology, Guizhou Normal University, Guiyang, Guizhou 550001, China.

ABSTRACT
104 samples from 27 accessions belonging to 12 species of genus Epimedium were studied on the basis of cytology observation, POD (i.e., peroxide) isozyme, high performance liquid chromatography (i.e., HPLC) fingerprint, and interspecific hybridization. The cytology observation showed karyotypes of twelve species studied; all are 2A symmetry type of Stebbins standard and similar to each other, and except for karyotype of E. leptorrhizum which is 2n = 2x = 8m (2SAT) + 4sm, the rest are 2n = 2x = 6m (2SAT) + 6sm. Chromosomes C-banding of barrenwort species varies, with 15 to 22 bands, consisting of centromeric bands, intercalary bands, terminal bands, and middle satellite bands. Results of POD isozyme showed that the zymographs vary greatly and sixteen bands were detected in the eleven species, and each species has its own characteristic bands different from the others. Studies on the HPLC fingerprint showed that the HPLC fingerprint of different species has characteristic peaks, divided into two regions (retention time < 10 min and retention time > 10 min). Results of interspecific hybridization showed that crosses of any combination among seven species studied are successful and the rates of grain set vary greatly. Based on these results, the system and phylogeny of this genus were inferred.

No MeSH data available.


Related in: MedlinePlus