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The cytology, isozyme, HPLC fingerprint, and interspecific hybridization studies of genus epimedium (berberidaceae).

Wang LJ, Sheng MY - ISRN Pharmacol (2013)

Bottom Line: Chromosomes C-banding of barrenwort species varies, with 15 to 22 bands, consisting of centromeric bands, intercalary bands, terminal bands, and middle satellite bands.Results of POD isozyme showed that the zymographs vary greatly and sixteen bands were detected in the eleven species, and each species has its own characteristic bands different from the others.Based on these results, the system and phylogeny of this genus were inferred.

View Article: PubMed Central - PubMed

Affiliation: School of Vocation and Technology, Guizhou Normal University, Guiyang, Guizhou 550001, China.

ABSTRACT
104 samples from 27 accessions belonging to 12 species of genus Epimedium were studied on the basis of cytology observation, POD (i.e., peroxide) isozyme, high performance liquid chromatography (i.e., HPLC) fingerprint, and interspecific hybridization. The cytology observation showed karyotypes of twelve species studied; all are 2A symmetry type of Stebbins standard and similar to each other, and except for karyotype of E. leptorrhizum which is 2n = 2x = 8m (2SAT) + 4sm, the rest are 2n = 2x = 6m (2SAT) + 6sm. Chromosomes C-banding of barrenwort species varies, with 15 to 22 bands, consisting of centromeric bands, intercalary bands, terminal bands, and middle satellite bands. Results of POD isozyme showed that the zymographs vary greatly and sixteen bands were detected in the eleven species, and each species has its own characteristic bands different from the others. Studies on the HPLC fingerprint showed that the HPLC fingerprint of different species has characteristic peaks, divided into two regions (retention time < 10 min and retention time > 10 min). Results of interspecific hybridization showed that crosses of any combination among seven species studied are successful and the rates of grain set vary greatly. Based on these results, the system and phylogeny of this genus were inferred.

No MeSH data available.


Related in: MedlinePlus

HPLC fingerprint of eight species. 1: E. wushanense. 2: E. sagittatum. 3: E. yinjiangense. 4: E. leptorrhizum. 5: E. simplicifolium. 6: E. luodianense. 7: E. davidii. 8: E. acuminatum.
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fig7: HPLC fingerprint of eight species. 1: E. wushanense. 2: E. sagittatum. 3: E. yinjiangense. 4: E. leptorrhizum. 5: E. simplicifolium. 6: E. luodianense. 7: E. davidii. 8: E. acuminatum.

Mentions: The flavonoids HPLC fingerprints of twenty-one barrenwort accessions, belonging to eight species were observed (Figure 7). The result showed that the HPLC fingerprint of different species were different. Each species had characteristic peaks, which were different from those seen in other species. All the HPLC fingerprint peaks can be divided into two regions: Region 1, retention time < 10 min, and Region 2, retention time > 10 min. Based on the number and location of peaks held by the species in Region 2, the eight species can be grouped into three types: Type 1 (E. sagittatum) with Peak 1–8, Type 2 (E. acuminatum, E. yinjiangense, E. wushanense, and E. simplicifolium) with Peak 1–5, and Type 3 (E. luodianense and E. leptorrhizum) without any peak, suggesting that the relation among E. acuminatum, E. yinjiangense, E. wushanense, E. simplicifolium, and E. davidii is near each other; E. luodianense and E. leptorrhizum are also near. E. sagittatum is distant from other species.


The cytology, isozyme, HPLC fingerprint, and interspecific hybridization studies of genus epimedium (berberidaceae).

Wang LJ, Sheng MY - ISRN Pharmacol (2013)

HPLC fingerprint of eight species. 1: E. wushanense. 2: E. sagittatum. 3: E. yinjiangense. 4: E. leptorrhizum. 5: E. simplicifolium. 6: E. luodianense. 7: E. davidii. 8: E. acuminatum.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3852721&req=5

fig7: HPLC fingerprint of eight species. 1: E. wushanense. 2: E. sagittatum. 3: E. yinjiangense. 4: E. leptorrhizum. 5: E. simplicifolium. 6: E. luodianense. 7: E. davidii. 8: E. acuminatum.
Mentions: The flavonoids HPLC fingerprints of twenty-one barrenwort accessions, belonging to eight species were observed (Figure 7). The result showed that the HPLC fingerprint of different species were different. Each species had characteristic peaks, which were different from those seen in other species. All the HPLC fingerprint peaks can be divided into two regions: Region 1, retention time < 10 min, and Region 2, retention time > 10 min. Based on the number and location of peaks held by the species in Region 2, the eight species can be grouped into three types: Type 1 (E. sagittatum) with Peak 1–8, Type 2 (E. acuminatum, E. yinjiangense, E. wushanense, and E. simplicifolium) with Peak 1–5, and Type 3 (E. luodianense and E. leptorrhizum) without any peak, suggesting that the relation among E. acuminatum, E. yinjiangense, E. wushanense, E. simplicifolium, and E. davidii is near each other; E. luodianense and E. leptorrhizum are also near. E. sagittatum is distant from other species.

Bottom Line: Chromosomes C-banding of barrenwort species varies, with 15 to 22 bands, consisting of centromeric bands, intercalary bands, terminal bands, and middle satellite bands.Results of POD isozyme showed that the zymographs vary greatly and sixteen bands were detected in the eleven species, and each species has its own characteristic bands different from the others.Based on these results, the system and phylogeny of this genus were inferred.

View Article: PubMed Central - PubMed

Affiliation: School of Vocation and Technology, Guizhou Normal University, Guiyang, Guizhou 550001, China.

ABSTRACT
104 samples from 27 accessions belonging to 12 species of genus Epimedium were studied on the basis of cytology observation, POD (i.e., peroxide) isozyme, high performance liquid chromatography (i.e., HPLC) fingerprint, and interspecific hybridization. The cytology observation showed karyotypes of twelve species studied; all are 2A symmetry type of Stebbins standard and similar to each other, and except for karyotype of E. leptorrhizum which is 2n = 2x = 8m (2SAT) + 4sm, the rest are 2n = 2x = 6m (2SAT) + 6sm. Chromosomes C-banding of barrenwort species varies, with 15 to 22 bands, consisting of centromeric bands, intercalary bands, terminal bands, and middle satellite bands. Results of POD isozyme showed that the zymographs vary greatly and sixteen bands were detected in the eleven species, and each species has its own characteristic bands different from the others. Studies on the HPLC fingerprint showed that the HPLC fingerprint of different species has characteristic peaks, divided into two regions (retention time < 10 min and retention time > 10 min). Results of interspecific hybridization showed that crosses of any combination among seven species studied are successful and the rates of grain set vary greatly. Based on these results, the system and phylogeny of this genus were inferred.

No MeSH data available.


Related in: MedlinePlus