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The cytology, isozyme, HPLC fingerprint, and interspecific hybridization studies of genus epimedium (berberidaceae).

Wang LJ, Sheng MY - ISRN Pharmacol (2013)

Bottom Line: Chromosomes C-banding of barrenwort species varies, with 15 to 22 bands, consisting of centromeric bands, intercalary bands, terminal bands, and middle satellite bands.Results of POD isozyme showed that the zymographs vary greatly and sixteen bands were detected in the eleven species, and each species has its own characteristic bands different from the others.Based on these results, the system and phylogeny of this genus were inferred.

View Article: PubMed Central - PubMed

Affiliation: School of Vocation and Technology, Guizhou Normal University, Guiyang, Guizhou 550001, China.

ABSTRACT
104 samples from 27 accessions belonging to 12 species of genus Epimedium were studied on the basis of cytology observation, POD (i.e., peroxide) isozyme, high performance liquid chromatography (i.e., HPLC) fingerprint, and interspecific hybridization. The cytology observation showed karyotypes of twelve species studied; all are 2A symmetry type of Stebbins standard and similar to each other, and except for karyotype of E. leptorrhizum which is 2n = 2x = 8m (2SAT) + 4sm, the rest are 2n = 2x = 6m (2SAT) + 6sm. Chromosomes C-banding of barrenwort species varies, with 15 to 22 bands, consisting of centromeric bands, intercalary bands, terminal bands, and middle satellite bands. Results of POD isozyme showed that the zymographs vary greatly and sixteen bands were detected in the eleven species, and each species has its own characteristic bands different from the others. Studies on the HPLC fingerprint showed that the HPLC fingerprint of different species has characteristic peaks, divided into two regions (retention time < 10 min and retention time > 10 min). Results of interspecific hybridization showed that crosses of any combination among seven species studied are successful and the rates of grain set vary greatly. Based on these results, the system and phylogeny of this genus were inferred.

No MeSH data available.


Related in: MedlinePlus

Idiograms of C-banded karyotypes in the eight species of genus Epimedium. 1: E. simplicifolium. 2: E. leptorrhizum. 3: E. wushanense. 4: E. alpinum. 5: E. davidii. 6: E. acuminatum. 7: E. pubigerum. 8: E. sagittatum.
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fig5: Idiograms of C-banded karyotypes in the eight species of genus Epimedium. 1: E. simplicifolium. 2: E. leptorrhizum. 3: E. wushanense. 4: E. alpinum. 5: E. davidii. 6: E. acuminatum. 7: E. pubigerum. 8: E. sagittatum.

Mentions: Eight species, E. sagittatum, E. alpinum, E. pubigerum, E. acuminatum, E. simplicifolium, E. wushanense, E. letorrhizum, and E. davidii, were treated by chromosomes C-banding. The metaphase chromosomes of C-banding, Giemsa C-banding karyotypes and idiograms of C-banding karyotype can be seen in Figures 3, 4, and 5, respectively.


The cytology, isozyme, HPLC fingerprint, and interspecific hybridization studies of genus epimedium (berberidaceae).

Wang LJ, Sheng MY - ISRN Pharmacol (2013)

Idiograms of C-banded karyotypes in the eight species of genus Epimedium. 1: E. simplicifolium. 2: E. leptorrhizum. 3: E. wushanense. 4: E. alpinum. 5: E. davidii. 6: E. acuminatum. 7: E. pubigerum. 8: E. sagittatum.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3852721&req=5

fig5: Idiograms of C-banded karyotypes in the eight species of genus Epimedium. 1: E. simplicifolium. 2: E. leptorrhizum. 3: E. wushanense. 4: E. alpinum. 5: E. davidii. 6: E. acuminatum. 7: E. pubigerum. 8: E. sagittatum.
Mentions: Eight species, E. sagittatum, E. alpinum, E. pubigerum, E. acuminatum, E. simplicifolium, E. wushanense, E. letorrhizum, and E. davidii, were treated by chromosomes C-banding. The metaphase chromosomes of C-banding, Giemsa C-banding karyotypes and idiograms of C-banding karyotype can be seen in Figures 3, 4, and 5, respectively.

Bottom Line: Chromosomes C-banding of barrenwort species varies, with 15 to 22 bands, consisting of centromeric bands, intercalary bands, terminal bands, and middle satellite bands.Results of POD isozyme showed that the zymographs vary greatly and sixteen bands were detected in the eleven species, and each species has its own characteristic bands different from the others.Based on these results, the system and phylogeny of this genus were inferred.

View Article: PubMed Central - PubMed

Affiliation: School of Vocation and Technology, Guizhou Normal University, Guiyang, Guizhou 550001, China.

ABSTRACT
104 samples from 27 accessions belonging to 12 species of genus Epimedium were studied on the basis of cytology observation, POD (i.e., peroxide) isozyme, high performance liquid chromatography (i.e., HPLC) fingerprint, and interspecific hybridization. The cytology observation showed karyotypes of twelve species studied; all are 2A symmetry type of Stebbins standard and similar to each other, and except for karyotype of E. leptorrhizum which is 2n = 2x = 8m (2SAT) + 4sm, the rest are 2n = 2x = 6m (2SAT) + 6sm. Chromosomes C-banding of barrenwort species varies, with 15 to 22 bands, consisting of centromeric bands, intercalary bands, terminal bands, and middle satellite bands. Results of POD isozyme showed that the zymographs vary greatly and sixteen bands were detected in the eleven species, and each species has its own characteristic bands different from the others. Studies on the HPLC fingerprint showed that the HPLC fingerprint of different species has characteristic peaks, divided into two regions (retention time < 10 min and retention time > 10 min). Results of interspecific hybridization showed that crosses of any combination among seven species studied are successful and the rates of grain set vary greatly. Based on these results, the system and phylogeny of this genus were inferred.

No MeSH data available.


Related in: MedlinePlus