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Leptin increases mitotic index and regeneration ratio in hepatectomized rats.

Cilekar M, Uysal O, Bal C, Turel S, Yılmaz S - Med Sci Monit Basic Res (2013)

Bottom Line: Group 1 was evaluated as the sham group and no surgical procedure was performed on animals.These groups were evaluated as the experiment groups at 24, 48, and 72 hours.Blood samples were collected for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and the remaining tissue samples were obtained for liver histopathology, regeneration rate, and mitotic index (MI).

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Eskişehir Osmangazi University, Medical Faculty, Eskisehir, Turkey.

ABSTRACT

Background: The aim of this study was to evaluate the potential effect of intraperitoneal administration of leptin on the hepatic regeneration and the mitotic index.

Material and methods: 56 Sprague-Dawley rats were divided into 7 groups each containing 8 rats. Group 1 was evaluated as the sham group and no surgical procedure was performed on animals. The rats in groups 2, 3, and 4 (named C24, C48, C72, respectively) were given intraperitoneal injection of 2 ml/kg normal saline 60 minutes before the surgical procedure consisting of laparotomy and 70% hepatectomy. These groups were used as controls at 24, 48, and 72 hours. The rats in groups 5, 6, and 7 (named L24, L48, and L72, respectively) were given intraperitoneal injection of 20 µg/kg doses of recombinant mouse leptin 60 minutes before the same surgical procedure. These groups were evaluated as the experiment groups at 24, 48, and 72 hours. Blood samples were collected for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and the remaining tissue samples were obtained for liver histopathology, regeneration rate, and mitotic index (MI). The weights of the remaining livers were also noted.

Results: The values of AST and ALT were higher in the groups that were administered leptin and they had significantly higher mitotic index than the other groups. Leptin also significantly increased the regeneration ratio as compared to the control group. The weights of the remaining livers were also higher in the leptin groups.

Conclusions: Intraperitoneal administration of leptin was observed to increase liver regeneration and mitotic rate in 70% hepatectomized rats.

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Histopathologic sections of the leptin groups at 24th (A), 48th (B) and 72nd (C) hours. (A) Diffuse replication and mitosis in the liver sections of L24 group. H&E. Bar 50 μm. (B) Hepatocytes at different phases of mitosis (arrow) in the liver sections of L48 group. H&E. Bar 50 μm. (C) Mitosis and reduced vacuolization of the hepatocytes in liver sections of L72 group. H&E. Bar 50 μm.
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f5-medscimonitbasicres-19-279: Histopathologic sections of the leptin groups at 24th (A), 48th (B) and 72nd (C) hours. (A) Diffuse replication and mitosis in the liver sections of L24 group. H&E. Bar 50 μm. (B) Hepatocytes at different phases of mitosis (arrow) in the liver sections of L48 group. H&E. Bar 50 μm. (C) Mitosis and reduced vacuolization of the hepatocytes in liver sections of L72 group. H&E. Bar 50 μm.

Mentions: A highly diffused replication was observed in the liver sections of the 24-hour leptin group (Figure 5A), whereas the liver sections of the 48-hour leptin group showed increased number of hepatocyte counts in different phases of mitosis (Figure 5B). The liver sections of the 72-hour leptin group demonstrated the progression of mitosis (Figure 5C). In all of the leptin groups, the vacuolization of the hepatocytes was partially reduced and polymorphonuclear cell infiltration was rarely observed.


Leptin increases mitotic index and regeneration ratio in hepatectomized rats.

Cilekar M, Uysal O, Bal C, Turel S, Yılmaz S - Med Sci Monit Basic Res (2013)

Histopathologic sections of the leptin groups at 24th (A), 48th (B) and 72nd (C) hours. (A) Diffuse replication and mitosis in the liver sections of L24 group. H&E. Bar 50 μm. (B) Hepatocytes at different phases of mitosis (arrow) in the liver sections of L48 group. H&E. Bar 50 μm. (C) Mitosis and reduced vacuolization of the hepatocytes in liver sections of L72 group. H&E. Bar 50 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3852623&req=5

f5-medscimonitbasicres-19-279: Histopathologic sections of the leptin groups at 24th (A), 48th (B) and 72nd (C) hours. (A) Diffuse replication and mitosis in the liver sections of L24 group. H&E. Bar 50 μm. (B) Hepatocytes at different phases of mitosis (arrow) in the liver sections of L48 group. H&E. Bar 50 μm. (C) Mitosis and reduced vacuolization of the hepatocytes in liver sections of L72 group. H&E. Bar 50 μm.
Mentions: A highly diffused replication was observed in the liver sections of the 24-hour leptin group (Figure 5A), whereas the liver sections of the 48-hour leptin group showed increased number of hepatocyte counts in different phases of mitosis (Figure 5B). The liver sections of the 72-hour leptin group demonstrated the progression of mitosis (Figure 5C). In all of the leptin groups, the vacuolization of the hepatocytes was partially reduced and polymorphonuclear cell infiltration was rarely observed.

Bottom Line: Group 1 was evaluated as the sham group and no surgical procedure was performed on animals.These groups were evaluated as the experiment groups at 24, 48, and 72 hours.Blood samples were collected for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and the remaining tissue samples were obtained for liver histopathology, regeneration rate, and mitotic index (MI).

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Eskişehir Osmangazi University, Medical Faculty, Eskisehir, Turkey.

ABSTRACT

Background: The aim of this study was to evaluate the potential effect of intraperitoneal administration of leptin on the hepatic regeneration and the mitotic index.

Material and methods: 56 Sprague-Dawley rats were divided into 7 groups each containing 8 rats. Group 1 was evaluated as the sham group and no surgical procedure was performed on animals. The rats in groups 2, 3, and 4 (named C24, C48, C72, respectively) were given intraperitoneal injection of 2 ml/kg normal saline 60 minutes before the surgical procedure consisting of laparotomy and 70% hepatectomy. These groups were used as controls at 24, 48, and 72 hours. The rats in groups 5, 6, and 7 (named L24, L48, and L72, respectively) were given intraperitoneal injection of 20 µg/kg doses of recombinant mouse leptin 60 minutes before the same surgical procedure. These groups were evaluated as the experiment groups at 24, 48, and 72 hours. Blood samples were collected for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and the remaining tissue samples were obtained for liver histopathology, regeneration rate, and mitotic index (MI). The weights of the remaining livers were also noted.

Results: The values of AST and ALT were higher in the groups that were administered leptin and they had significantly higher mitotic index than the other groups. Leptin also significantly increased the regeneration ratio as compared to the control group. The weights of the remaining livers were also higher in the leptin groups.

Conclusions: Intraperitoneal administration of leptin was observed to increase liver regeneration and mitotic rate in 70% hepatectomized rats.

Show MeSH
Related in: MedlinePlus