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Leptin increases mitotic index and regeneration ratio in hepatectomized rats.

Cilekar M, Uysal O, Bal C, Turel S, Yılmaz S - Med Sci Monit Basic Res (2013)

Bottom Line: Group 1 was evaluated as the sham group and no surgical procedure was performed on animals.These groups were evaluated as the experiment groups at 24, 48, and 72 hours.Blood samples were collected for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and the remaining tissue samples were obtained for liver histopathology, regeneration rate, and mitotic index (MI).

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Eskişehir Osmangazi University, Medical Faculty, Eskisehir, Turkey.

ABSTRACT

Background: The aim of this study was to evaluate the potential effect of intraperitoneal administration of leptin on the hepatic regeneration and the mitotic index.

Material and methods: 56 Sprague-Dawley rats were divided into 7 groups each containing 8 rats. Group 1 was evaluated as the sham group and no surgical procedure was performed on animals. The rats in groups 2, 3, and 4 (named C24, C48, C72, respectively) were given intraperitoneal injection of 2 ml/kg normal saline 60 minutes before the surgical procedure consisting of laparotomy and 70% hepatectomy. These groups were used as controls at 24, 48, and 72 hours. The rats in groups 5, 6, and 7 (named L24, L48, and L72, respectively) were given intraperitoneal injection of 20 µg/kg doses of recombinant mouse leptin 60 minutes before the same surgical procedure. These groups were evaluated as the experiment groups at 24, 48, and 72 hours. Blood samples were collected for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and the remaining tissue samples were obtained for liver histopathology, regeneration rate, and mitotic index (MI). The weights of the remaining livers were also noted.

Results: The values of AST and ALT were higher in the groups that were administered leptin and they had significantly higher mitotic index than the other groups. Leptin also significantly increased the regeneration ratio as compared to the control group. The weights of the remaining livers were also higher in the leptin groups.

Conclusions: Intraperitoneal administration of leptin was observed to increase liver regeneration and mitotic rate in 70% hepatectomized rats.

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Related in: MedlinePlus

Transaminase values of the groups. Leptin produced a transient elevation in AST (A) and ALT (B) levels however they returned to similar levels as control ones at 3rd day.
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f1-medscimonitbasicres-19-279: Transaminase values of the groups. Leptin produced a transient elevation in AST (A) and ALT (B) levels however they returned to similar levels as control ones at 3rd day.

Mentions: The biochemical analyses shows that there was a statistically significant difference (p<0.01) between the sham and the other groups, revealing that hepatectomy of 70% produced significant parenchymal injury in terms of increased AST and ALT levels (IU/L). However, the values of the control and leptin groups revealed that leptin produced more pronounced injury until 48 hours, but these levels returned to similar values as control at 72 hours. There was no statistically significant difference (p>0.05) established between the 72-hour leptin group (L72) and the control group (C72) in respect to AST and ALT values (Figure 1).


Leptin increases mitotic index and regeneration ratio in hepatectomized rats.

Cilekar M, Uysal O, Bal C, Turel S, Yılmaz S - Med Sci Monit Basic Res (2013)

Transaminase values of the groups. Leptin produced a transient elevation in AST (A) and ALT (B) levels however they returned to similar levels as control ones at 3rd day.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3852623&req=5

f1-medscimonitbasicres-19-279: Transaminase values of the groups. Leptin produced a transient elevation in AST (A) and ALT (B) levels however they returned to similar levels as control ones at 3rd day.
Mentions: The biochemical analyses shows that there was a statistically significant difference (p<0.01) between the sham and the other groups, revealing that hepatectomy of 70% produced significant parenchymal injury in terms of increased AST and ALT levels (IU/L). However, the values of the control and leptin groups revealed that leptin produced more pronounced injury until 48 hours, but these levels returned to similar values as control at 72 hours. There was no statistically significant difference (p>0.05) established between the 72-hour leptin group (L72) and the control group (C72) in respect to AST and ALT values (Figure 1).

Bottom Line: Group 1 was evaluated as the sham group and no surgical procedure was performed on animals.These groups were evaluated as the experiment groups at 24, 48, and 72 hours.Blood samples were collected for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and the remaining tissue samples were obtained for liver histopathology, regeneration rate, and mitotic index (MI).

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Eskişehir Osmangazi University, Medical Faculty, Eskisehir, Turkey.

ABSTRACT

Background: The aim of this study was to evaluate the potential effect of intraperitoneal administration of leptin on the hepatic regeneration and the mitotic index.

Material and methods: 56 Sprague-Dawley rats were divided into 7 groups each containing 8 rats. Group 1 was evaluated as the sham group and no surgical procedure was performed on animals. The rats in groups 2, 3, and 4 (named C24, C48, C72, respectively) were given intraperitoneal injection of 2 ml/kg normal saline 60 minutes before the surgical procedure consisting of laparotomy and 70% hepatectomy. These groups were used as controls at 24, 48, and 72 hours. The rats in groups 5, 6, and 7 (named L24, L48, and L72, respectively) were given intraperitoneal injection of 20 µg/kg doses of recombinant mouse leptin 60 minutes before the same surgical procedure. These groups were evaluated as the experiment groups at 24, 48, and 72 hours. Blood samples were collected for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and the remaining tissue samples were obtained for liver histopathology, regeneration rate, and mitotic index (MI). The weights of the remaining livers were also noted.

Results: The values of AST and ALT were higher in the groups that were administered leptin and they had significantly higher mitotic index than the other groups. Leptin also significantly increased the regeneration ratio as compared to the control group. The weights of the remaining livers were also higher in the leptin groups.

Conclusions: Intraperitoneal administration of leptin was observed to increase liver regeneration and mitotic rate in 70% hepatectomized rats.

Show MeSH
Related in: MedlinePlus