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A possible role for CD8+ T lymphocytes in the cell-mediated pathogenesis of pemphigus vulgaris.

Giurdanella F, Fania L, Gnarra M, Toto P, Di Rollo D, Sauder DN, Feliciani C - Mediators Inflamm. (2013)

Bottom Line: Pemphigus vulgaris (PV) is an autoimmune blistering disease whose pathogenesis involves both humoral and cell-mediated immune response.The results of the immunohistochemical staining to study the expression of CD3, CD4, and CD8 in PV skin lesions showed that CD4+ are more expressed than CD8+ in the inflammatory infiltrate of PV lesions, confirming the data of the previous literature.These results suggest that CD8+ T cells may play a role in the pathogenesis of PV, perhaps through the Fas/FasL pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Policlinico A. Gemelli Hospital, Catholic University of the Sacred Heart, Largo Agostino Gemelli 8, 00168 Rome, Italy.

ABSTRACT
Pemphigus vulgaris (PV) is an autoimmune blistering disease whose pathogenesis involves both humoral and cell-mediated immune response. Though the pathogenetic role of autoantibodies directed against desmoglein 3 is certain, a number of other factors have been suggested to determine acantholysis in PV. In this study we examined the possible role of CD8+ T cells in the development of acantholysis by a passive transfer of PV autoantibodies using CD8 deficient mice, and we also studied the inflammatory infiltrate of PV skin lesions by immunohistochemical staining. The results of the immunohistochemical staining to study the expression of CD3, CD4, and CD8 in PV skin lesions showed that CD4+ are more expressed than CD8+ in the inflammatory infiltrate of PV lesions, confirming the data of the previous literature. The passive transfer study showed a lower incidence of pemphigus in the group of CD8 deficient mice compared to the control one of wild-type mice. These results suggest that CD8+ T cells may play a role in the pathogenesis of PV, perhaps through the Fas/FasL pathway.

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Related in: MedlinePlus

Incidence of disease in KO mice and control-C57BL/6 mice in passive transfer model of IgG PV. With a dose of 30 μL/g PV plasma, 0% (0/5) of the CD8−/− mice developed PV, compared with 9.5% (4/42) of C57BL/6 mice. With an injected dose of 50 μL/g, 44% (13/29) of the CD8−/− mice showed PV lesions, compared with 72% (26/36) of the control group.
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fig2: Incidence of disease in KO mice and control-C57BL/6 mice in passive transfer model of IgG PV. With a dose of 30 μL/g PV plasma, 0% (0/5) of the CD8−/− mice developed PV, compared with 9.5% (4/42) of C57BL/6 mice. With an injected dose of 50 μL/g, 44% (13/29) of the CD8−/− mice showed PV lesions, compared with 72% (26/36) of the control group.

Mentions: When a dose-response study was performed on CD8−/− mice, a lower incidence of pemphigus was observed. In particular, with a dose of 30 μL/g PV plasma, 0% (0/5) of the CD8−/− mice injected showed evidence of disease as compared with 9.5% (4/42) of C57BL/6 mice (difference statistically not significant). With an injected dose of 50 μL/g, 44% (13/29) of the CD8−/− mice developed PV lesions, compared with 72% (26/36) of the control group (difference statistically significant) (Figure 2).


A possible role for CD8+ T lymphocytes in the cell-mediated pathogenesis of pemphigus vulgaris.

Giurdanella F, Fania L, Gnarra M, Toto P, Di Rollo D, Sauder DN, Feliciani C - Mediators Inflamm. (2013)

Incidence of disease in KO mice and control-C57BL/6 mice in passive transfer model of IgG PV. With a dose of 30 μL/g PV plasma, 0% (0/5) of the CD8−/− mice developed PV, compared with 9.5% (4/42) of C57BL/6 mice. With an injected dose of 50 μL/g, 44% (13/29) of the CD8−/− mice showed PV lesions, compared with 72% (26/36) of the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3852314&req=5

fig2: Incidence of disease in KO mice and control-C57BL/6 mice in passive transfer model of IgG PV. With a dose of 30 μL/g PV plasma, 0% (0/5) of the CD8−/− mice developed PV, compared with 9.5% (4/42) of C57BL/6 mice. With an injected dose of 50 μL/g, 44% (13/29) of the CD8−/− mice showed PV lesions, compared with 72% (26/36) of the control group.
Mentions: When a dose-response study was performed on CD8−/− mice, a lower incidence of pemphigus was observed. In particular, with a dose of 30 μL/g PV plasma, 0% (0/5) of the CD8−/− mice injected showed evidence of disease as compared with 9.5% (4/42) of C57BL/6 mice (difference statistically not significant). With an injected dose of 50 μL/g, 44% (13/29) of the CD8−/− mice developed PV lesions, compared with 72% (26/36) of the control group (difference statistically significant) (Figure 2).

Bottom Line: Pemphigus vulgaris (PV) is an autoimmune blistering disease whose pathogenesis involves both humoral and cell-mediated immune response.The results of the immunohistochemical staining to study the expression of CD3, CD4, and CD8 in PV skin lesions showed that CD4+ are more expressed than CD8+ in the inflammatory infiltrate of PV lesions, confirming the data of the previous literature.These results suggest that CD8+ T cells may play a role in the pathogenesis of PV, perhaps through the Fas/FasL pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Policlinico A. Gemelli Hospital, Catholic University of the Sacred Heart, Largo Agostino Gemelli 8, 00168 Rome, Italy.

ABSTRACT
Pemphigus vulgaris (PV) is an autoimmune blistering disease whose pathogenesis involves both humoral and cell-mediated immune response. Though the pathogenetic role of autoantibodies directed against desmoglein 3 is certain, a number of other factors have been suggested to determine acantholysis in PV. In this study we examined the possible role of CD8+ T cells in the development of acantholysis by a passive transfer of PV autoantibodies using CD8 deficient mice, and we also studied the inflammatory infiltrate of PV skin lesions by immunohistochemical staining. The results of the immunohistochemical staining to study the expression of CD3, CD4, and CD8 in PV skin lesions showed that CD4+ are more expressed than CD8+ in the inflammatory infiltrate of PV lesions, confirming the data of the previous literature. The passive transfer study showed a lower incidence of pemphigus in the group of CD8 deficient mice compared to the control one of wild-type mice. These results suggest that CD8+ T cells may play a role in the pathogenesis of PV, perhaps through the Fas/FasL pathway.

Show MeSH
Related in: MedlinePlus