Limits...
Electroacupuncture regulates apoptosis/proliferation of intramuscular interstitial cells of cajal and restores colonic motility in diabetic constipation rats.

Xu J, Chen Y, Liu S, Hou X - Evid Based Complement Alternat Med (2013)

Bottom Line: Apoptotic ICC was detected by terminal dUTP nucleotide end labeling.Proliferating ICC was identified by Kit/Ki67 double immunofluorescent staining on whole mount preparations.Our results indicate that high-frequency EAS has stimulatory effect on the distal colonic transit, which may be mediated by downregulation of the apoptosis and upregulation of the proliferation of intramuscular ICC.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Road, Wuhan, Hubei 430022, China.

ABSTRACT
Injury of interstitial cells of Cajal (ICC) is associated with gut dysmotility in diabetic rats. We have shown an acceleration of the colonic contractility by electroacupuncture stimulation (EAS). However, little is known about potential roles of EAS on colonic transit and ICC. In this study, we evaluate the effect of EAS on colonic transit and investigate whether apoptosis/proliferation of ICC was involved in regulative effect of EAS on colonic transit. Rats were randomly assigned to normal, diabetic, diabetic-plus-sham stimulation, diabetic-plus-low-frequency stimulation, and diabetic-plus-high-frequency stimulation groups. Bead expulsion test was used for measuring the distal colonic transit. The Kit (ICC marker) was detected by western blot. Apoptotic ICC was detected by terminal dUTP nucleotide end labeling. Proliferating ICC was identified by Kit/Ki67 double immunofluorescent staining on whole mount preparations. Ultrastructure changes of ICC were studied using electron microscopy. Results showed that high-frequency stimulation significantly promoted colonic transit. Low- and high-frequency stimulation markedly rescued intramuscular ICC from apoptosis. Abundant proliferating intramuscular ICC was found in low- and high-frequency stimulation groups. Our results indicate that high-frequency EAS has stimulatory effect on the distal colonic transit, which may be mediated by downregulation of the apoptosis and upregulation of the proliferation of intramuscular ICC.

No MeSH data available.


Related in: MedlinePlus

Ultrastructure of ICC. Comparing with the control group, ICC was seriously injured in the DM and SEA group, while they showed nearly normal structure and minor injury in the LEA and HEA group. ICC: interstitial cells of Cajal; SMC: smooth muscle cell; NF: nerve fibre.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3852313&req=5

fig6: Ultrastructure of ICC. Comparing with the control group, ICC was seriously injured in the DM and SEA group, while they showed nearly normal structure and minor injury in the LEA and HEA group. ICC: interstitial cells of Cajal; SMC: smooth muscle cell; NF: nerve fibre.

Mentions: In the control group, ICC-IM had a higher electron-density cytoplasm compared with smooth muscle cells and was rich in cell organelles, including mitochondria, endoplasmic reticulum, and basal lamina (Figure 6(a)). They also displayed close connection with enteric nerves and smooth muscle cells. However, ICC-IM in the DM group was markedly affected (Figure 6(b)): swollen mitochondria with disrupted cristae, lamellar bodies, and partial cytoplasmic depletion were frequently present within the cell bodies or processes of ICC-IM. Most of them lost connection with enteric nerves. ICC-IM in the SEA group displayed the same ultrastructural abnormalities as in the DM group (Figure 6(c)). In contrast, most ICC-IM in the LEA (Figure 6(d)) and HEA groups (Figure 6(e)) were restored largely, and occasionally minor injury could be seen.


Electroacupuncture regulates apoptosis/proliferation of intramuscular interstitial cells of cajal and restores colonic motility in diabetic constipation rats.

Xu J, Chen Y, Liu S, Hou X - Evid Based Complement Alternat Med (2013)

Ultrastructure of ICC. Comparing with the control group, ICC was seriously injured in the DM and SEA group, while they showed nearly normal structure and minor injury in the LEA and HEA group. ICC: interstitial cells of Cajal; SMC: smooth muscle cell; NF: nerve fibre.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3852313&req=5

fig6: Ultrastructure of ICC. Comparing with the control group, ICC was seriously injured in the DM and SEA group, while they showed nearly normal structure and minor injury in the LEA and HEA group. ICC: interstitial cells of Cajal; SMC: smooth muscle cell; NF: nerve fibre.
Mentions: In the control group, ICC-IM had a higher electron-density cytoplasm compared with smooth muscle cells and was rich in cell organelles, including mitochondria, endoplasmic reticulum, and basal lamina (Figure 6(a)). They also displayed close connection with enteric nerves and smooth muscle cells. However, ICC-IM in the DM group was markedly affected (Figure 6(b)): swollen mitochondria with disrupted cristae, lamellar bodies, and partial cytoplasmic depletion were frequently present within the cell bodies or processes of ICC-IM. Most of them lost connection with enteric nerves. ICC-IM in the SEA group displayed the same ultrastructural abnormalities as in the DM group (Figure 6(c)). In contrast, most ICC-IM in the LEA (Figure 6(d)) and HEA groups (Figure 6(e)) were restored largely, and occasionally minor injury could be seen.

Bottom Line: Apoptotic ICC was detected by terminal dUTP nucleotide end labeling.Proliferating ICC was identified by Kit/Ki67 double immunofluorescent staining on whole mount preparations.Our results indicate that high-frequency EAS has stimulatory effect on the distal colonic transit, which may be mediated by downregulation of the apoptosis and upregulation of the proliferation of intramuscular ICC.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Road, Wuhan, Hubei 430022, China.

ABSTRACT
Injury of interstitial cells of Cajal (ICC) is associated with gut dysmotility in diabetic rats. We have shown an acceleration of the colonic contractility by electroacupuncture stimulation (EAS). However, little is known about potential roles of EAS on colonic transit and ICC. In this study, we evaluate the effect of EAS on colonic transit and investigate whether apoptosis/proliferation of ICC was involved in regulative effect of EAS on colonic transit. Rats were randomly assigned to normal, diabetic, diabetic-plus-sham stimulation, diabetic-plus-low-frequency stimulation, and diabetic-plus-high-frequency stimulation groups. Bead expulsion test was used for measuring the distal colonic transit. The Kit (ICC marker) was detected by western blot. Apoptotic ICC was detected by terminal dUTP nucleotide end labeling. Proliferating ICC was identified by Kit/Ki67 double immunofluorescent staining on whole mount preparations. Ultrastructure changes of ICC were studied using electron microscopy. Results showed that high-frequency stimulation significantly promoted colonic transit. Low- and high-frequency stimulation markedly rescued intramuscular ICC from apoptosis. Abundant proliferating intramuscular ICC was found in low- and high-frequency stimulation groups. Our results indicate that high-frequency EAS has stimulatory effect on the distal colonic transit, which may be mediated by downregulation of the apoptosis and upregulation of the proliferation of intramuscular ICC.

No MeSH data available.


Related in: MedlinePlus