Limits...
Electroacupuncture regulates apoptosis/proliferation of intramuscular interstitial cells of cajal and restores colonic motility in diabetic constipation rats.

Xu J, Chen Y, Liu S, Hou X - Evid Based Complement Alternat Med (2013)

Bottom Line: Apoptotic ICC was detected by terminal dUTP nucleotide end labeling.Proliferating ICC was identified by Kit/Ki67 double immunofluorescent staining on whole mount preparations.Our results indicate that high-frequency EAS has stimulatory effect on the distal colonic transit, which may be mediated by downregulation of the apoptosis and upregulation of the proliferation of intramuscular ICC.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Road, Wuhan, Hubei 430022, China.

ABSTRACT
Injury of interstitial cells of Cajal (ICC) is associated with gut dysmotility in diabetic rats. We have shown an acceleration of the colonic contractility by electroacupuncture stimulation (EAS). However, little is known about potential roles of EAS on colonic transit and ICC. In this study, we evaluate the effect of EAS on colonic transit and investigate whether apoptosis/proliferation of ICC was involved in regulative effect of EAS on colonic transit. Rats were randomly assigned to normal, diabetic, diabetic-plus-sham stimulation, diabetic-plus-low-frequency stimulation, and diabetic-plus-high-frequency stimulation groups. Bead expulsion test was used for measuring the distal colonic transit. The Kit (ICC marker) was detected by western blot. Apoptotic ICC was detected by terminal dUTP nucleotide end labeling. Proliferating ICC was identified by Kit/Ki67 double immunofluorescent staining on whole mount preparations. Ultrastructure changes of ICC were studied using electron microscopy. Results showed that high-frequency stimulation significantly promoted colonic transit. Low- and high-frequency stimulation markedly rescued intramuscular ICC from apoptosis. Abundant proliferating intramuscular ICC was found in low- and high-frequency stimulation groups. Our results indicate that high-frequency EAS has stimulatory effect on the distal colonic transit, which may be mediated by downregulation of the apoptosis and upregulation of the proliferation of intramuscular ICC.

No MeSH data available.


Related in: MedlinePlus

Western blot analysis of Kit in colon tissue which the submucosa removed. Compared with the control group, the expression of Kit was decreased in the DM group; there were no significant difference between the DM and SEA group (P > 0.05). However, they were increased markedly in the LEA and HEA group. (*Significantly different to the DM group, #significantly different to the LEA group.)
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3852313&req=5

fig3: Western blot analysis of Kit in colon tissue which the submucosa removed. Compared with the control group, the expression of Kit was decreased in the DM group; there were no significant difference between the DM and SEA group (P > 0.05). However, they were increased markedly in the LEA and HEA group. (*Significantly different to the DM group, #significantly different to the LEA group.)

Mentions: In contrast to the control group, the expression of Kit in the DM group was decreased markedly (P < 0.01). Similarly, no significance was detected between the SEA and DM group (P > 0.05). In the LEA group, the expression of Kit showed a significant increase (P < 0.01), while, in the HEA group, an enhancement was found nearly four times higher than the DM group. The increased level of Kit expression in the HEA group was somewhat higher than the LEA group, and the difference was statistically significant (P < 0.01) (Figure 3).


Electroacupuncture regulates apoptosis/proliferation of intramuscular interstitial cells of cajal and restores colonic motility in diabetic constipation rats.

Xu J, Chen Y, Liu S, Hou X - Evid Based Complement Alternat Med (2013)

Western blot analysis of Kit in colon tissue which the submucosa removed. Compared with the control group, the expression of Kit was decreased in the DM group; there were no significant difference between the DM and SEA group (P > 0.05). However, they were increased markedly in the LEA and HEA group. (*Significantly different to the DM group, #significantly different to the LEA group.)
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3852313&req=5

fig3: Western blot analysis of Kit in colon tissue which the submucosa removed. Compared with the control group, the expression of Kit was decreased in the DM group; there were no significant difference between the DM and SEA group (P > 0.05). However, they were increased markedly in the LEA and HEA group. (*Significantly different to the DM group, #significantly different to the LEA group.)
Mentions: In contrast to the control group, the expression of Kit in the DM group was decreased markedly (P < 0.01). Similarly, no significance was detected between the SEA and DM group (P > 0.05). In the LEA group, the expression of Kit showed a significant increase (P < 0.01), while, in the HEA group, an enhancement was found nearly four times higher than the DM group. The increased level of Kit expression in the HEA group was somewhat higher than the LEA group, and the difference was statistically significant (P < 0.01) (Figure 3).

Bottom Line: Apoptotic ICC was detected by terminal dUTP nucleotide end labeling.Proliferating ICC was identified by Kit/Ki67 double immunofluorescent staining on whole mount preparations.Our results indicate that high-frequency EAS has stimulatory effect on the distal colonic transit, which may be mediated by downregulation of the apoptosis and upregulation of the proliferation of intramuscular ICC.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Road, Wuhan, Hubei 430022, China.

ABSTRACT
Injury of interstitial cells of Cajal (ICC) is associated with gut dysmotility in diabetic rats. We have shown an acceleration of the colonic contractility by electroacupuncture stimulation (EAS). However, little is known about potential roles of EAS on colonic transit and ICC. In this study, we evaluate the effect of EAS on colonic transit and investigate whether apoptosis/proliferation of ICC was involved in regulative effect of EAS on colonic transit. Rats were randomly assigned to normal, diabetic, diabetic-plus-sham stimulation, diabetic-plus-low-frequency stimulation, and diabetic-plus-high-frequency stimulation groups. Bead expulsion test was used for measuring the distal colonic transit. The Kit (ICC marker) was detected by western blot. Apoptotic ICC was detected by terminal dUTP nucleotide end labeling. Proliferating ICC was identified by Kit/Ki67 double immunofluorescent staining on whole mount preparations. Ultrastructure changes of ICC were studied using electron microscopy. Results showed that high-frequency stimulation significantly promoted colonic transit. Low- and high-frequency stimulation markedly rescued intramuscular ICC from apoptosis. Abundant proliferating intramuscular ICC was found in low- and high-frequency stimulation groups. Our results indicate that high-frequency EAS has stimulatory effect on the distal colonic transit, which may be mediated by downregulation of the apoptosis and upregulation of the proliferation of intramuscular ICC.

No MeSH data available.


Related in: MedlinePlus