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Electroacupuncture regulates apoptosis/proliferation of intramuscular interstitial cells of cajal and restores colonic motility in diabetic constipation rats.

Xu J, Chen Y, Liu S, Hou X - Evid Based Complement Alternat Med (2013)

Bottom Line: Apoptotic ICC was detected by terminal dUTP nucleotide end labeling.Proliferating ICC was identified by Kit/Ki67 double immunofluorescent staining on whole mount preparations.Our results indicate that high-frequency EAS has stimulatory effect on the distal colonic transit, which may be mediated by downregulation of the apoptosis and upregulation of the proliferation of intramuscular ICC.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Road, Wuhan, Hubei 430022, China.

ABSTRACT
Injury of interstitial cells of Cajal (ICC) is associated with gut dysmotility in diabetic rats. We have shown an acceleration of the colonic contractility by electroacupuncture stimulation (EAS). However, little is known about potential roles of EAS on colonic transit and ICC. In this study, we evaluate the effect of EAS on colonic transit and investigate whether apoptosis/proliferation of ICC was involved in regulative effect of EAS on colonic transit. Rats were randomly assigned to normal, diabetic, diabetic-plus-sham stimulation, diabetic-plus-low-frequency stimulation, and diabetic-plus-high-frequency stimulation groups. Bead expulsion test was used for measuring the distal colonic transit. The Kit (ICC marker) was detected by western blot. Apoptotic ICC was detected by terminal dUTP nucleotide end labeling. Proliferating ICC was identified by Kit/Ki67 double immunofluorescent staining on whole mount preparations. Ultrastructure changes of ICC were studied using electron microscopy. Results showed that high-frequency stimulation significantly promoted colonic transit. Low- and high-frequency stimulation markedly rescued intramuscular ICC from apoptosis. Abundant proliferating intramuscular ICC was found in low- and high-frequency stimulation groups. Our results indicate that high-frequency EAS has stimulatory effect on the distal colonic transit, which may be mediated by downregulation of the apoptosis and upregulation of the proliferation of intramuscular ICC.

No MeSH data available.


Related in: MedlinePlus

Effects of EAS at ST-36 on distal colonic transit. In contrast to the control, the DM group displayed delayed colonic transit, while EAS with high-frequency at ST-36 significantly increased colonic transit. The colonic transit time was not changed obviously in the SEA and LEA group. Data are given as means ± SEM; an asterisk indicates statistical significance at a level of P < 0.05. (*Significantly different to the DM group for 4 weeks, #significantly different to the DM group for 8 weeks.)
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fig2: Effects of EAS at ST-36 on distal colonic transit. In contrast to the control, the DM group displayed delayed colonic transit, while EAS with high-frequency at ST-36 significantly increased colonic transit. The colonic transit time was not changed obviously in the SEA and LEA group. Data are given as means ± SEM; an asterisk indicates statistical significance at a level of P < 0.05. (*Significantly different to the DM group for 4 weeks, #significantly different to the DM group for 8 weeks.)

Mentions: As shown in Figure 2, no differences were noted in the baseline colonic transit time among the five groups. At the end of 4 and 8 weeks, the distal colonic transit in the DM group was delayed significantly (122.0 ± 6.4 min versus 16.1 ± 3.2 min, P < 0.01; 265.7 ± 23.9 min versus 15.1 ± 4.5 min, P < 0.01). In the SEA and LEA group, the distal colonic motility was not significantly altered compared with the DM group for 4 weeks (119.0 ± 9.3 min versus 122.0 ± 6.4 min, P > 0.05; 118.6 ± 9.3 min versus 122.0 ± 6.4 min, P > 0.05) and 8 weeks (263.6 ± 25.9 min versus 265.7 ± 23.9 min, P > 0.05; 243.2 ± 21.3 min versus 265.7 ± 23.9 min, P > 0.05), respectively. However, the HEA at ST-36 significantly promoted the distal colonic motility for 4 and 8 weeks, respectively (36.7 ± 6.6 min versus 122.0 ± 6.4 min, P < 0.01; 43.1 ± 4.2 min versus 265.7 ± 23.9 min, P < 0.01).


Electroacupuncture regulates apoptosis/proliferation of intramuscular interstitial cells of cajal and restores colonic motility in diabetic constipation rats.

Xu J, Chen Y, Liu S, Hou X - Evid Based Complement Alternat Med (2013)

Effects of EAS at ST-36 on distal colonic transit. In contrast to the control, the DM group displayed delayed colonic transit, while EAS with high-frequency at ST-36 significantly increased colonic transit. The colonic transit time was not changed obviously in the SEA and LEA group. Data are given as means ± SEM; an asterisk indicates statistical significance at a level of P < 0.05. (*Significantly different to the DM group for 4 weeks, #significantly different to the DM group for 8 weeks.)
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3852313&req=5

fig2: Effects of EAS at ST-36 on distal colonic transit. In contrast to the control, the DM group displayed delayed colonic transit, while EAS with high-frequency at ST-36 significantly increased colonic transit. The colonic transit time was not changed obviously in the SEA and LEA group. Data are given as means ± SEM; an asterisk indicates statistical significance at a level of P < 0.05. (*Significantly different to the DM group for 4 weeks, #significantly different to the DM group for 8 weeks.)
Mentions: As shown in Figure 2, no differences were noted in the baseline colonic transit time among the five groups. At the end of 4 and 8 weeks, the distal colonic transit in the DM group was delayed significantly (122.0 ± 6.4 min versus 16.1 ± 3.2 min, P < 0.01; 265.7 ± 23.9 min versus 15.1 ± 4.5 min, P < 0.01). In the SEA and LEA group, the distal colonic motility was not significantly altered compared with the DM group for 4 weeks (119.0 ± 9.3 min versus 122.0 ± 6.4 min, P > 0.05; 118.6 ± 9.3 min versus 122.0 ± 6.4 min, P > 0.05) and 8 weeks (263.6 ± 25.9 min versus 265.7 ± 23.9 min, P > 0.05; 243.2 ± 21.3 min versus 265.7 ± 23.9 min, P > 0.05), respectively. However, the HEA at ST-36 significantly promoted the distal colonic motility for 4 and 8 weeks, respectively (36.7 ± 6.6 min versus 122.0 ± 6.4 min, P < 0.01; 43.1 ± 4.2 min versus 265.7 ± 23.9 min, P < 0.01).

Bottom Line: Apoptotic ICC was detected by terminal dUTP nucleotide end labeling.Proliferating ICC was identified by Kit/Ki67 double immunofluorescent staining on whole mount preparations.Our results indicate that high-frequency EAS has stimulatory effect on the distal colonic transit, which may be mediated by downregulation of the apoptosis and upregulation of the proliferation of intramuscular ICC.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Road, Wuhan, Hubei 430022, China.

ABSTRACT
Injury of interstitial cells of Cajal (ICC) is associated with gut dysmotility in diabetic rats. We have shown an acceleration of the colonic contractility by electroacupuncture stimulation (EAS). However, little is known about potential roles of EAS on colonic transit and ICC. In this study, we evaluate the effect of EAS on colonic transit and investigate whether apoptosis/proliferation of ICC was involved in regulative effect of EAS on colonic transit. Rats were randomly assigned to normal, diabetic, diabetic-plus-sham stimulation, diabetic-plus-low-frequency stimulation, and diabetic-plus-high-frequency stimulation groups. Bead expulsion test was used for measuring the distal colonic transit. The Kit (ICC marker) was detected by western blot. Apoptotic ICC was detected by terminal dUTP nucleotide end labeling. Proliferating ICC was identified by Kit/Ki67 double immunofluorescent staining on whole mount preparations. Ultrastructure changes of ICC were studied using electron microscopy. Results showed that high-frequency stimulation significantly promoted colonic transit. Low- and high-frequency stimulation markedly rescued intramuscular ICC from apoptosis. Abundant proliferating intramuscular ICC was found in low- and high-frequency stimulation groups. Our results indicate that high-frequency EAS has stimulatory effect on the distal colonic transit, which may be mediated by downregulation of the apoptosis and upregulation of the proliferation of intramuscular ICC.

No MeSH data available.


Related in: MedlinePlus