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Differential Expression of Myogenic Regulatory Factor Genes in the Skeletal Muscles of Tambaqui Colossoma macropomum (Cuvier 1818) from Amazonian Black and Clear Water.

Alves-Costa FA, Barbosa CM, Aguiar RC, Mareco EA, Dal-Pai-Silva M - Int J Genomics (2013)

Bottom Line: All of the MRF transcript levels (myod, myf5, myogenin, and mrf4) were significantly lower in the red muscle from black water fish in comparison to clear water fish.However, in white muscle, only the myod transcript level was significantly decreased in the black water tambaqui.Our results showed that the physical and chemical water characteristics change the expression of genes that promote muscle growth, and these results may be also widely applicable to future projects that aim to enhance muscle growth in fish that are of substantial interest to the aquaculture.

View Article: PubMed Central - PubMed

Affiliation: Universidade Paulista (UNIP), Instituto de Ciências da Saúde, R. Luiz Levorato 20108, 17048-290 Bauru, SP, Brazil.

ABSTRACT
Hypothesizing that the Amazonian water system differences would affect the expression of muscle growth-related genes in juvenile tambaqui Colossoma macropomum (Cuvier 1818), this study aimed to analyze the morphometric data and expression of myogenic regulatory factors (MRFs) in the white and red muscle from tambaqui obtained from clear and black Amazonian water systems. All of the MRF transcript levels (myod, myf5, myogenin, and mrf4) were significantly lower in the red muscle from black water fish in comparison to clear water fish. However, in white muscle, only the myod transcript level was significantly decreased in the black water tambaqui. The changes in MRFs gene expression in muscle fibers of tambaqui from black water system provide relevant information about the environmental influence as that of water systems on gene expression of muscle growth related genes in the C. macropomum. Our results showed that the physical and chemical water characteristics change the expression of genes that promote muscle growth, and these results may be also widely applicable to future projects that aim to enhance muscle growth in fish that are of substantial interest to the aquaculture.

No MeSH data available.


Distribution (a) and frequency (b) of white muscle fibers of Colossoma macropomum from clear and black Amazon waters, according to the diameter class classification.
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Related In: Results  -  Collection


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fig1: Distribution (a) and frequency (b) of white muscle fibers of Colossoma macropomum from clear and black Amazon waters, according to the diameter class classification.

Mentions: The same pattern of fiber diameters distribution was observed when the white muscle fibers from clear water fish were compared with the muscle fibers from the black water fish. Moreover, the percentages of fibers in each diameter class were similar in the white muscle tissues of both types of fish (Figure 1). It is worth noting that the average lengths of the specimens from the two different aquatic environments were equally similar. The clear water specimens presented a mean length of approximately nine centimeters, and the black water specimens presented a mean length of approximately seven centimeters.


Differential Expression of Myogenic Regulatory Factor Genes in the Skeletal Muscles of Tambaqui Colossoma macropomum (Cuvier 1818) from Amazonian Black and Clear Water.

Alves-Costa FA, Barbosa CM, Aguiar RC, Mareco EA, Dal-Pai-Silva M - Int J Genomics (2013)

Distribution (a) and frequency (b) of white muscle fibers of Colossoma macropomum from clear and black Amazon waters, according to the diameter class classification.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3852311&req=5

fig1: Distribution (a) and frequency (b) of white muscle fibers of Colossoma macropomum from clear and black Amazon waters, according to the diameter class classification.
Mentions: The same pattern of fiber diameters distribution was observed when the white muscle fibers from clear water fish were compared with the muscle fibers from the black water fish. Moreover, the percentages of fibers in each diameter class were similar in the white muscle tissues of both types of fish (Figure 1). It is worth noting that the average lengths of the specimens from the two different aquatic environments were equally similar. The clear water specimens presented a mean length of approximately nine centimeters, and the black water specimens presented a mean length of approximately seven centimeters.

Bottom Line: All of the MRF transcript levels (myod, myf5, myogenin, and mrf4) were significantly lower in the red muscle from black water fish in comparison to clear water fish.However, in white muscle, only the myod transcript level was significantly decreased in the black water tambaqui.Our results showed that the physical and chemical water characteristics change the expression of genes that promote muscle growth, and these results may be also widely applicable to future projects that aim to enhance muscle growth in fish that are of substantial interest to the aquaculture.

View Article: PubMed Central - PubMed

Affiliation: Universidade Paulista (UNIP), Instituto de Ciências da Saúde, R. Luiz Levorato 20108, 17048-290 Bauru, SP, Brazil.

ABSTRACT
Hypothesizing that the Amazonian water system differences would affect the expression of muscle growth-related genes in juvenile tambaqui Colossoma macropomum (Cuvier 1818), this study aimed to analyze the morphometric data and expression of myogenic regulatory factors (MRFs) in the white and red muscle from tambaqui obtained from clear and black Amazonian water systems. All of the MRF transcript levels (myod, myf5, myogenin, and mrf4) were significantly lower in the red muscle from black water fish in comparison to clear water fish. However, in white muscle, only the myod transcript level was significantly decreased in the black water tambaqui. The changes in MRFs gene expression in muscle fibers of tambaqui from black water system provide relevant information about the environmental influence as that of water systems on gene expression of muscle growth related genes in the C. macropomum. Our results showed that the physical and chemical water characteristics change the expression of genes that promote muscle growth, and these results may be also widely applicable to future projects that aim to enhance muscle growth in fish that are of substantial interest to the aquaculture.

No MeSH data available.