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Anti-CEA-functionalized superparamagnetic iron oxide nanoparticles for examining colorectal tumors in vivo.

Huang KW, Chieh JJ, Lin IT, Horng HE, Yang HC, Hong CY - Nanoscale Res Lett (2013)

Bottom Line: However, because MRI requires a nonmetal environment, it was limited to preoperative imaging.Furthermore, biological methodologies of different tissue staining methods and inductively coupled plasma (ICP) yielded consistent results, proving that the obtained in vivo results occurred because of targeted anti-CEA SPIONPs.This indicates that developed anti-CEA SPIONPs owe the utilities as an image medium of these in vivo methodologies.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan. jjchieh@ntnu.edu.tw.

ABSTRACT
Although the biomarker carcinoembryonic antigen (CEA) is expressed in colorectal tumors, the utility of an anti-CEA-functionalized image medium is powerful for in vivo positioning of colorectal tumors. With a risk of superparamagnetic iron oxide nanoparticles (SPIONPs) that is lower for animals than other material carriers, anti-CEA-functionalized SPIONPs were synthesized in this study for labeling colorectal tumors by conducting different preoperatively and intraoperatively in vivo examinations. In magnetic resonance imaging (MRI), the image variation of colorectal tumors reached the maximum at approximately 24 h. However, because MRI requires a nonmetal environment, it was limited to preoperative imaging. With the potentiality of in vivo screening and intraoperative positioning during surgery, the scanning superconducting-quantum-interference-device biosusceptometry (SSB) was adopted, showing the favorable agreement of time-varied intensity with MRI. Furthermore, biological methodologies of different tissue staining methods and inductively coupled plasma (ICP) yielded consistent results, proving that the obtained in vivo results occurred because of targeted anti-CEA SPIONPs. This indicates that developed anti-CEA SPIONPs owe the utilities as an image medium of these in vivo methodologies.

No MeSH data available.


Related in: MedlinePlus

Biological results of the tumors of mouse 3, mouse 4, and mouse 5. (a) Tissue staining methods of HE staining, PB staining, anti-CEA staining, and CD 31 staining. (b) Iron amount by ICP. The circles are data points obtained from the measured results of two tissues.
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Figure 5: Biological results of the tumors of mouse 3, mouse 4, and mouse 5. (a) Tissue staining methods of HE staining, PB staining, anti-CEA staining, and CD 31 staining. (b) Iron amount by ICP. The circles are data points obtained from the measured results of two tissues.

Mentions: Furthermore, these magnetic characteristics of a tumor labeled with anti-CEA SPIONPs were verified using the gold standard of biological assays, tumor tissue staining, and ICP. FigureĀ 5a shows the times at which the colorectal tumors were removed from mouse 3 (at the 0th hour), mouse 4 (at the 24th hour), and mouse 5 (at the 98th hour), by PB staining, immunostaining of anti-CEA protein, CD 31 staining, and HE staining. As shown in the photo of HE staining, these cells also developed in proximity to disorganized architectures because of the increased ratio of nuclei to cytoplasm. This indicated that these tissues were obtained from tumors. Furthermore, there are significant blue spots (arrows), representative of iron elements, in the PB photo and brown spots (arrows) in the anti-CEA and CD 31 photos at the 24th hour, but not at the 0th and 98th hours. In addition, the distribution consistency of the blue spots in the PB photos, as well as the brown spots in both the anti-CEA and CD31 photos, indicated that the tumors were labeled by these anti-CEA SPIONPs rather than by biodegraded iron ions through the transportation of microvessels. This also confirmed that selecting the upper tumor region was more suitable than selecting the entire tumor for MRI because of the live zone of the tumor with both microvessels and anti-CEA SPIONPs.


Anti-CEA-functionalized superparamagnetic iron oxide nanoparticles for examining colorectal tumors in vivo.

Huang KW, Chieh JJ, Lin IT, Horng HE, Yang HC, Hong CY - Nanoscale Res Lett (2013)

Biological results of the tumors of mouse 3, mouse 4, and mouse 5. (a) Tissue staining methods of HE staining, PB staining, anti-CEA staining, and CD 31 staining. (b) Iron amount by ICP. The circles are data points obtained from the measured results of two tissues.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3852279&req=5

Figure 5: Biological results of the tumors of mouse 3, mouse 4, and mouse 5. (a) Tissue staining methods of HE staining, PB staining, anti-CEA staining, and CD 31 staining. (b) Iron amount by ICP. The circles are data points obtained from the measured results of two tissues.
Mentions: Furthermore, these magnetic characteristics of a tumor labeled with anti-CEA SPIONPs were verified using the gold standard of biological assays, tumor tissue staining, and ICP. FigureĀ 5a shows the times at which the colorectal tumors were removed from mouse 3 (at the 0th hour), mouse 4 (at the 24th hour), and mouse 5 (at the 98th hour), by PB staining, immunostaining of anti-CEA protein, CD 31 staining, and HE staining. As shown in the photo of HE staining, these cells also developed in proximity to disorganized architectures because of the increased ratio of nuclei to cytoplasm. This indicated that these tissues were obtained from tumors. Furthermore, there are significant blue spots (arrows), representative of iron elements, in the PB photo and brown spots (arrows) in the anti-CEA and CD 31 photos at the 24th hour, but not at the 0th and 98th hours. In addition, the distribution consistency of the blue spots in the PB photos, as well as the brown spots in both the anti-CEA and CD31 photos, indicated that the tumors were labeled by these anti-CEA SPIONPs rather than by biodegraded iron ions through the transportation of microvessels. This also confirmed that selecting the upper tumor region was more suitable than selecting the entire tumor for MRI because of the live zone of the tumor with both microvessels and anti-CEA SPIONPs.

Bottom Line: However, because MRI requires a nonmetal environment, it was limited to preoperative imaging.Furthermore, biological methodologies of different tissue staining methods and inductively coupled plasma (ICP) yielded consistent results, proving that the obtained in vivo results occurred because of targeted anti-CEA SPIONPs.This indicates that developed anti-CEA SPIONPs owe the utilities as an image medium of these in vivo methodologies.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan. jjchieh@ntnu.edu.tw.

ABSTRACT
Although the biomarker carcinoembryonic antigen (CEA) is expressed in colorectal tumors, the utility of an anti-CEA-functionalized image medium is powerful for in vivo positioning of colorectal tumors. With a risk of superparamagnetic iron oxide nanoparticles (SPIONPs) that is lower for animals than other material carriers, anti-CEA-functionalized SPIONPs were synthesized in this study for labeling colorectal tumors by conducting different preoperatively and intraoperatively in vivo examinations. In magnetic resonance imaging (MRI), the image variation of colorectal tumors reached the maximum at approximately 24 h. However, because MRI requires a nonmetal environment, it was limited to preoperative imaging. With the potentiality of in vivo screening and intraoperative positioning during surgery, the scanning superconducting-quantum-interference-device biosusceptometry (SSB) was adopted, showing the favorable agreement of time-varied intensity with MRI. Furthermore, biological methodologies of different tissue staining methods and inductively coupled plasma (ICP) yielded consistent results, proving that the obtained in vivo results occurred because of targeted anti-CEA SPIONPs. This indicates that developed anti-CEA SPIONPs owe the utilities as an image medium of these in vivo methodologies.

No MeSH data available.


Related in: MedlinePlus