Limits...
RNA-Seq analysis of Citrus reticulata in the early stages of Xylella fastidiosa infection reveals auxin-related genes as a defense response.

Rodrigues CM, de Souza AA, Takita MA, Kishi LT, Machado MA - BMC Genomics (2013)

Bottom Line: A set of genes considered key elements in the resistance was used to confirm its regulation in mandarin compared with the susceptible sweet orange.Gene expression analysis of mock inoculated and infected tissues of Ponkan mandarin identified 667 transcripts repressed and 724 significantly induced in the later.We also hypothesized the induction of auxin-related genes indicates that resistant plants initially recognize X. fastidiosa as a necrotrophic pathogen.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Biotecnologia, Centro APTA Citros Sylvio Moreira, CP4, Cordeirópolis, SP 13490-970, Brazil. marcos@centrodecitricultura.br.

ABSTRACT

Background: Citrus variegated chlorosis (CVC), caused by Xylella fastidiosa, is one the most important citrus diseases, and affects all varieties of sweet orange (Citrus sinensis L. Osb). On the other hand, among the Citrus genus there are different sources of resistance against X. fastidiosa. For these species identifying these defense genes could be an important step towards obtaining sweet orange resistant varieties through breeding or genetic engineering. To assess these genes we made use of mandarin (C. reticulata Blanco) that is known to be resistant to CVC and shares agronomical characteristics with sweet orange. Thus, we investigated the gene expression in Ponkan mandarin at one day after infection with X. fastidiosa, using RNA-seq. A set of genes considered key elements in the resistance was used to confirm its regulation in mandarin compared with the susceptible sweet orange.

Results: Gene expression analysis of mock inoculated and infected tissues of Ponkan mandarin identified 667 transcripts repressed and 724 significantly induced in the later. Among the induced transcripts, we identified genes encoding proteins similar to Pattern Recognition Receptors. Furthermore, many genes involved in secondary metabolism, biosynthesis and cell wall modification were upregulated as well as in synthesis of abscisic acid, jasmonic acid and auxin.

Conclusions: This work demonstrated that the defense response to the perception of bacteria involves cell wall modification and activation of hormone pathways, which probably lead to the induction of other defense-related genes. We also hypothesized the induction of auxin-related genes indicates that resistant plants initially recognize X. fastidiosa as a necrotrophic pathogen.

Show MeSH

Related in: MedlinePlus

Responses related to secondary metabolism and the cell wall in Ponkan mandarin 1 day after infection with X. fastidiosa. Log2 fold change of gene expression (X. fastidiosa versus mock inoculated control) was analyzed by MapMan. Blue squares represent upregulated genes, red squares represent downregulated genes and grey circles represent not differentially regulated genes. The color saturation indicates fold change >0.8 and < -0.8. GSL07 (Ciclev10030560m), glucan synthase-like 7; CSLA09 (Ciclev10031284m), cellulose synthase like A9; CESA8 (Ciclev10014155m), cellulose synthase; CESA4 (Ciclev10018639m), cellulose synthase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3852278&req=5

Figure 2: Responses related to secondary metabolism and the cell wall in Ponkan mandarin 1 day after infection with X. fastidiosa. Log2 fold change of gene expression (X. fastidiosa versus mock inoculated control) was analyzed by MapMan. Blue squares represent upregulated genes, red squares represent downregulated genes and grey circles represent not differentially regulated genes. The color saturation indicates fold change >0.8 and < -0.8. GSL07 (Ciclev10030560m), glucan synthase-like 7; CSLA09 (Ciclev10031284m), cellulose synthase like A9; CESA8 (Ciclev10014155m), cellulose synthase; CESA4 (Ciclev10018639m), cellulose synthase.

Mentions: In this study, we observed a significant change in expression of genes involved in secondary metabolism, and cell wall biosynthesis and modification in Ponkan mandarin infected with X. fastidiosa. These genes were mapped using MapMan to generate a representative overview (Figure 2 and Additional file 3).


RNA-Seq analysis of Citrus reticulata in the early stages of Xylella fastidiosa infection reveals auxin-related genes as a defense response.

Rodrigues CM, de Souza AA, Takita MA, Kishi LT, Machado MA - BMC Genomics (2013)

Responses related to secondary metabolism and the cell wall in Ponkan mandarin 1 day after infection with X. fastidiosa. Log2 fold change of gene expression (X. fastidiosa versus mock inoculated control) was analyzed by MapMan. Blue squares represent upregulated genes, red squares represent downregulated genes and grey circles represent not differentially regulated genes. The color saturation indicates fold change >0.8 and < -0.8. GSL07 (Ciclev10030560m), glucan synthase-like 7; CSLA09 (Ciclev10031284m), cellulose synthase like A9; CESA8 (Ciclev10014155m), cellulose synthase; CESA4 (Ciclev10018639m), cellulose synthase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3852278&req=5

Figure 2: Responses related to secondary metabolism and the cell wall in Ponkan mandarin 1 day after infection with X. fastidiosa. Log2 fold change of gene expression (X. fastidiosa versus mock inoculated control) was analyzed by MapMan. Blue squares represent upregulated genes, red squares represent downregulated genes and grey circles represent not differentially regulated genes. The color saturation indicates fold change >0.8 and < -0.8. GSL07 (Ciclev10030560m), glucan synthase-like 7; CSLA09 (Ciclev10031284m), cellulose synthase like A9; CESA8 (Ciclev10014155m), cellulose synthase; CESA4 (Ciclev10018639m), cellulose synthase.
Mentions: In this study, we observed a significant change in expression of genes involved in secondary metabolism, and cell wall biosynthesis and modification in Ponkan mandarin infected with X. fastidiosa. These genes were mapped using MapMan to generate a representative overview (Figure 2 and Additional file 3).

Bottom Line: A set of genes considered key elements in the resistance was used to confirm its regulation in mandarin compared with the susceptible sweet orange.Gene expression analysis of mock inoculated and infected tissues of Ponkan mandarin identified 667 transcripts repressed and 724 significantly induced in the later.We also hypothesized the induction of auxin-related genes indicates that resistant plants initially recognize X. fastidiosa as a necrotrophic pathogen.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Biotecnologia, Centro APTA Citros Sylvio Moreira, CP4, Cordeirópolis, SP 13490-970, Brazil. marcos@centrodecitricultura.br.

ABSTRACT

Background: Citrus variegated chlorosis (CVC), caused by Xylella fastidiosa, is one the most important citrus diseases, and affects all varieties of sweet orange (Citrus sinensis L. Osb). On the other hand, among the Citrus genus there are different sources of resistance against X. fastidiosa. For these species identifying these defense genes could be an important step towards obtaining sweet orange resistant varieties through breeding or genetic engineering. To assess these genes we made use of mandarin (C. reticulata Blanco) that is known to be resistant to CVC and shares agronomical characteristics with sweet orange. Thus, we investigated the gene expression in Ponkan mandarin at one day after infection with X. fastidiosa, using RNA-seq. A set of genes considered key elements in the resistance was used to confirm its regulation in mandarin compared with the susceptible sweet orange.

Results: Gene expression analysis of mock inoculated and infected tissues of Ponkan mandarin identified 667 transcripts repressed and 724 significantly induced in the later. Among the induced transcripts, we identified genes encoding proteins similar to Pattern Recognition Receptors. Furthermore, many genes involved in secondary metabolism, biosynthesis and cell wall modification were upregulated as well as in synthesis of abscisic acid, jasmonic acid and auxin.

Conclusions: This work demonstrated that the defense response to the perception of bacteria involves cell wall modification and activation of hormone pathways, which probably lead to the induction of other defense-related genes. We also hypothesized the induction of auxin-related genes indicates that resistant plants initially recognize X. fastidiosa as a necrotrophic pathogen.

Show MeSH
Related in: MedlinePlus