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Prognostic implication of neuropilin-1 upregulation in human nasopharyngeal carcinoma.

Xu Y, Li P, Zhang X, Wang J, Gu D, Wang Y - Diagn Pathol (2013)

Bottom Line: In addition, high NRP-1 expression was significantly associated with advanced clinical stage (P = 0.02), positive recurrence (P = 0.001) and metastasis status (P = 0.001) of NPC.Moreover, the NPC patients with higher NRP-1 expression had shorter overall survival, whereas patients with lower NRP-1 expression had better survival (P < 0.001).These findings suggest for the first time that NRP-1 upregulation may be a novel biomarker for the prediction of advanced tumor progression and unfavorable prognosis in NPC patients who may benefit from alternative treatment strategy and targeted treatment.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Otorhinolaryngology, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an, Jiangsu 223300, P, R, China. xuyuhayy@163.com.

ABSTRACT

Objective: As a receptor for both vascular endothelial growth factors and semaphorin, neuropilin-1 (NRP-1) is reported to be up-regulated in cells of several cancers. However, its roles in human nasopharyngeal carcinoma (NPC) are still unclear. Therefore, the goal of this study was to investigate the expression pattern of NRP-1 in NPC tissues, to clarify the clinical significance of NRP-1 expression in NPC as well as the potential prognostic implication of NRP-1 expression.

Methods: Immunohistochemistry was performed to detect the expression of NRP-1 in tumor tissue samples from 266 NPC patients. The association of NRP-1 protein expression with the clinicopathological characteristics and the prognosis of NPC were subsequently assessed.

Results: Immunohistochemical analysis showed that 176 of 266 (66.17%) paraffin-embedded archival NPC biopsies showed high expression of NRP-1, but no non-cancerous nasopharyngeal specimens showed positive expression of NRP-1. In addition, high NRP-1 expression was significantly associated with advanced clinical stage (P = 0.02), positive recurrence (P = 0.001) and metastasis status (P = 0.001) of NPC. Moreover, the NPC patients with higher NRP-1 expression had shorter overall survival, whereas patients with lower NRP-1 expression had better survival (P < 0.001). Furthermore, the multivariate analysis indicated that the overexpression of NRP-1 protein was an independent prognostic factor for overall survival (P = 0.001) in NPC patients.

Conclusion: These findings suggest for the first time that NRP-1 upregulation may be a novel biomarker for the prediction of advanced tumor progression and unfavorable prognosis in NPC patients who may benefit from alternative treatment strategy and targeted treatment.

Virtual slides: The virtual slides for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1507827881105018.

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Immunohistochemical staining of NRP-1 protein in tumor cells of nasopharyngeal carcinoma (NPC, A) and non-cancerous nasopharyngeal tissues (B; Original magnification × 400). Intense staining of NRP-1 is seen in the cell membrane and/or cytoplasm of tumors cells and is intensive in NPC tissues (A). In contrast, negative immunostainings of NRP-1 (B) was observed in non-cancerous nasopharyngeal tissues.
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Figure 1: Immunohistochemical staining of NRP-1 protein in tumor cells of nasopharyngeal carcinoma (NPC, A) and non-cancerous nasopharyngeal tissues (B; Original magnification × 400). Intense staining of NRP-1 is seen in the cell membrane and/or cytoplasm of tumors cells and is intensive in NPC tissues (A). In contrast, negative immunostainings of NRP-1 (B) was observed in non-cancerous nasopharyngeal tissues.

Mentions: Expression and subcellular localization of NRP-1 protein was detected by immunohistochemistry in 266 NPC specimens and 100 non-cancerous nasopharyngeal specimens. The coefficient of variation of interobserver reproducibility was 9.62% for NRP-1 expression scoring, with no significant differences between the two observers (paired t-test, P = 0.82). As shown in Figure 1, the cell membrane and/or cytoplasm of most tumor cells in the NPC sections stained intensely with NRP-1 antibody (Figure 1A), while negative immunostainings were observed in all non-cancerous nasopharyngeal tissues (Figure 1B). High NRP-1 expression was observed in tumor cells of 66.17% (176/266) NPCs.


Prognostic implication of neuropilin-1 upregulation in human nasopharyngeal carcinoma.

Xu Y, Li P, Zhang X, Wang J, Gu D, Wang Y - Diagn Pathol (2013)

Immunohistochemical staining of NRP-1 protein in tumor cells of nasopharyngeal carcinoma (NPC, A) and non-cancerous nasopharyngeal tissues (B; Original magnification × 400). Intense staining of NRP-1 is seen in the cell membrane and/or cytoplasm of tumors cells and is intensive in NPC tissues (A). In contrast, negative immunostainings of NRP-1 (B) was observed in non-cancerous nasopharyngeal tissues.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3852274&req=5

Figure 1: Immunohistochemical staining of NRP-1 protein in tumor cells of nasopharyngeal carcinoma (NPC, A) and non-cancerous nasopharyngeal tissues (B; Original magnification × 400). Intense staining of NRP-1 is seen in the cell membrane and/or cytoplasm of tumors cells and is intensive in NPC tissues (A). In contrast, negative immunostainings of NRP-1 (B) was observed in non-cancerous nasopharyngeal tissues.
Mentions: Expression and subcellular localization of NRP-1 protein was detected by immunohistochemistry in 266 NPC specimens and 100 non-cancerous nasopharyngeal specimens. The coefficient of variation of interobserver reproducibility was 9.62% for NRP-1 expression scoring, with no significant differences between the two observers (paired t-test, P = 0.82). As shown in Figure 1, the cell membrane and/or cytoplasm of most tumor cells in the NPC sections stained intensely with NRP-1 antibody (Figure 1A), while negative immunostainings were observed in all non-cancerous nasopharyngeal tissues (Figure 1B). High NRP-1 expression was observed in tumor cells of 66.17% (176/266) NPCs.

Bottom Line: In addition, high NRP-1 expression was significantly associated with advanced clinical stage (P = 0.02), positive recurrence (P = 0.001) and metastasis status (P = 0.001) of NPC.Moreover, the NPC patients with higher NRP-1 expression had shorter overall survival, whereas patients with lower NRP-1 expression had better survival (P < 0.001).These findings suggest for the first time that NRP-1 upregulation may be a novel biomarker for the prediction of advanced tumor progression and unfavorable prognosis in NPC patients who may benefit from alternative treatment strategy and targeted treatment.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Otorhinolaryngology, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an, Jiangsu 223300, P, R, China. xuyuhayy@163.com.

ABSTRACT

Objective: As a receptor for both vascular endothelial growth factors and semaphorin, neuropilin-1 (NRP-1) is reported to be up-regulated in cells of several cancers. However, its roles in human nasopharyngeal carcinoma (NPC) are still unclear. Therefore, the goal of this study was to investigate the expression pattern of NRP-1 in NPC tissues, to clarify the clinical significance of NRP-1 expression in NPC as well as the potential prognostic implication of NRP-1 expression.

Methods: Immunohistochemistry was performed to detect the expression of NRP-1 in tumor tissue samples from 266 NPC patients. The association of NRP-1 protein expression with the clinicopathological characteristics and the prognosis of NPC were subsequently assessed.

Results: Immunohistochemical analysis showed that 176 of 266 (66.17%) paraffin-embedded archival NPC biopsies showed high expression of NRP-1, but no non-cancerous nasopharyngeal specimens showed positive expression of NRP-1. In addition, high NRP-1 expression was significantly associated with advanced clinical stage (P = 0.02), positive recurrence (P = 0.001) and metastasis status (P = 0.001) of NPC. Moreover, the NPC patients with higher NRP-1 expression had shorter overall survival, whereas patients with lower NRP-1 expression had better survival (P < 0.001). Furthermore, the multivariate analysis indicated that the overexpression of NRP-1 protein was an independent prognostic factor for overall survival (P = 0.001) in NPC patients.

Conclusion: These findings suggest for the first time that NRP-1 upregulation may be a novel biomarker for the prediction of advanced tumor progression and unfavorable prognosis in NPC patients who may benefit from alternative treatment strategy and targeted treatment.

Virtual slides: The virtual slides for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1507827881105018.

Show MeSH
Related in: MedlinePlus