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Regulatory roles of the PI3K/Akt signaling pathway in rats with severe acute pancreatitis.

Xu P, Wang J, Yang ZW, Lou XL, Chen C - PLoS ONE (2013)

Bottom Line: The phosphatidylinositol 3-kinase(PI3K)/protein kinase B (Akt) pathway plays a key role in inflammation.The SAP group was found to have a significant increase in pancreas Akt expression, along with the activation of serum amylase, TNF-α, IL-1β, and IL-6, and pancreas histological aggravation.The administration of wortmannin in SAP rats reduced Akt expression, attenuated the level of serum amylase and inflammation factor, and alleviated the damage of pancreatic tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Songjiang Hospital Affiliated the First People's Hospital, Shanghai Jiaotong University, Shanghai, China.

ABSTRACT
The phosphatidylinositol 3-kinase(PI3K)/protein kinase B (Akt) pathway plays a key role in inflammation. However, the regulatory roles of PI3K/Akt in severe acute pancreatitis (SAP) have not been elucidated. The aim of this study was to investigate the impact of wortmannin, a PI3K/Akt inhibitor, on SAP rats through exposure to sodium taurocholate (STC) after 3 h and 6 h. The SAP group was found to have a significant increase in pancreas Akt expression, along with the activation of serum amylase, TNF-α, IL-1β, and IL-6, and pancreas histological aggravation. The administration of wortmannin in SAP rats reduced Akt expression, attenuated the level of serum amylase and inflammation factor, and alleviated the damage of pancreatic tissue. Furthermore, the administration of wortmannin led to an obvious reduction in NF-κB and p38MAPK expression in SAP rats. These findings showed that the PI3K/Akt inhibitor wortmannin decreases inflammatory cytokines in SAP rats and suggests its regulatory mechanisms may occur through the suppression on NF-κB and p38MAPK activity.

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Related in: MedlinePlus

Akt mRNA expression in each group at the 3 h and 6 h time point.#P<0.05 vs. SO group; ★P<0.05 vs. SAP group.
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pone-0081767-g009: Akt mRNA expression in each group at the 3 h and 6 h time point.#P<0.05 vs. SO group; ★P<0.05 vs. SAP group.


Regulatory roles of the PI3K/Akt signaling pathway in rats with severe acute pancreatitis.

Xu P, Wang J, Yang ZW, Lou XL, Chen C - PLoS ONE (2013)

Akt mRNA expression in each group at the 3 h and 6 h time point.#P<0.05 vs. SO group; ★P<0.05 vs. SAP group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3842964&req=5

pone-0081767-g009: Akt mRNA expression in each group at the 3 h and 6 h time point.#P<0.05 vs. SO group; ★P<0.05 vs. SAP group.
Bottom Line: The phosphatidylinositol 3-kinase(PI3K)/protein kinase B (Akt) pathway plays a key role in inflammation.The SAP group was found to have a significant increase in pancreas Akt expression, along with the activation of serum amylase, TNF-α, IL-1β, and IL-6, and pancreas histological aggravation.The administration of wortmannin in SAP rats reduced Akt expression, attenuated the level of serum amylase and inflammation factor, and alleviated the damage of pancreatic tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Songjiang Hospital Affiliated the First People's Hospital, Shanghai Jiaotong University, Shanghai, China.

ABSTRACT
The phosphatidylinositol 3-kinase(PI3K)/protein kinase B (Akt) pathway plays a key role in inflammation. However, the regulatory roles of PI3K/Akt in severe acute pancreatitis (SAP) have not been elucidated. The aim of this study was to investigate the impact of wortmannin, a PI3K/Akt inhibitor, on SAP rats through exposure to sodium taurocholate (STC) after 3 h and 6 h. The SAP group was found to have a significant increase in pancreas Akt expression, along with the activation of serum amylase, TNF-α, IL-1β, and IL-6, and pancreas histological aggravation. The administration of wortmannin in SAP rats reduced Akt expression, attenuated the level of serum amylase and inflammation factor, and alleviated the damage of pancreatic tissue. Furthermore, the administration of wortmannin led to an obvious reduction in NF-κB and p38MAPK expression in SAP rats. These findings showed that the PI3K/Akt inhibitor wortmannin decreases inflammatory cytokines in SAP rats and suggests its regulatory mechanisms may occur through the suppression on NF-κB and p38MAPK activity.

Show MeSH
Related in: MedlinePlus