Dickkopf-related protein 3 promotes pathogenic stromal remodeling in benign prostatic hyperplasia and prostate cancer.
Bottom Line: DKK3 knockdown did not affect subcellular localization or levels of β-catenin but attenuated AKT phosphorylation in PrSCs.Consistently the PI3K/AKT inhibitor LY294002 mimicked the effects of DKK3 knockdown.Therefore, Dkk-3 represents a potential therapeutic target for stromal remodeling in BPH and PCa.
Affiliation: Institute for Biomedical Aging Research, University of Innsbruck, Innsbruck, Austria. firstname.lastname@example.orgShow MeSH
Related in: MedlinePlus
Mentions: Primary prostatic stromal cells (PrSCs) were used to investigate the functional significance of Dkk-3 in the stromal compartment in vitro. Consistent with the predominant expression of Dkk-3 in the epithelial compartment of the benign prostate 1, Dkk-3 was more abundant in cell lysates from primary prostatic epithelial cells (PrECs) than PrSCs at the protein level as determined by Western blot analysis (Fig. 1A), however PrSCs secreted Dkk-3 at significant levels (Fig. 1C).
Affiliation: Institute for Biomedical Aging Research, University of Innsbruck, Innsbruck, Austria. email@example.com