Dickkopf-related protein 3 promotes pathogenic stromal remodeling in benign prostatic hyperplasia and prostate cancer.
Bottom Line: Knockdown of DKK3 significantly attenuated PrSC proliferation as well as fibroblast-to-myofibroblast differentiation and increased the expression of the vessel stabilizing factor angiopoietin-1.DKK3 knockdown did not affect subcellular localization or levels of β-catenin but attenuated AKT phosphorylation in PrSCs.Consistently the PI3K/AKT inhibitor LY294002 mimicked the effects of DKK3 knockdown.
Affiliation: Institute for Biomedical Aging Research, University of Innsbruck, Innsbruck, Austria. email@example.comShow MeSH
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Mentions: Primary prostatic stromal cells (PrSCs) were used to investigate the functional significance of Dkk-3 in the stromal compartment in vitro. Consistent with the predominant expression of Dkk-3 in the epithelial compartment of the benign prostate 1, Dkk-3 was more abundant in cell lysates from primary prostatic epithelial cells (PrECs) than PrSCs at the protein level as determined by Western blot analysis (Fig. 1A), however PrSCs secreted Dkk-3 at significant levels (Fig. 1C).
Affiliation: Institute for Biomedical Aging Research, University of Innsbruck, Innsbruck, Austria. firstname.lastname@example.org