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Screening of MicroRNA as potential CardiomiRs in Rattus noveregicus Heart related Dataset.

Rustagi Y, Rani V - Bioinformation (2013)

Bottom Line: MicroRNAs (miRNAs) are the naturally expressed small, 18~25 nts long non-coding single stranded RNAs, which inhibit the translation by interacting with the 3' untranslated region (UTR) of specific mRNA targets or by repression of posttranscriptional modification of mRNAs.Their role and association with several disease progressions is of interest in recent years.Therefore, we analyzed their features using a dataset (# ≈1400 #) of potential intronic and 3'UTR targeted miRNAs from known cardiac marker genes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, 201307, Uttar Pradesh, India.

ABSTRACT
MicroRNAs (miRNAs) are the naturally expressed small, 18~25 nts long non-coding single stranded RNAs, which inhibit the translation by interacting with the 3' untranslated region (UTR) of specific mRNA targets or by repression of posttranscriptional modification of mRNAs. MiRNAs are found to regulate the differentiation, development, function and stress responsive growth of cardiac cells. Their role and association with several disease progressions is of interest in recent years. Our interest is to study their role in cardiac hypertrophy (characterized by increased cell size, protein synthesis and reactivation of gene pathways). Therefore, we analyzed their features using a dataset (# ≈1400 #) of potential intronic and 3'UTR targeted miRNAs from known cardiac marker genes. We report 10 uncharacterized miRNAs regulating cardiac marker genes during cardiac hypertrophy and other cardiac diseases.

No MeSH data available.


Related in: MedlinePlus

Possible Role of screened microRNAs in cardiachypertrophy. During cardiac hypertrophy normal heartincreases in size due to re-expression of cardiac marker genes(shown in white boxes). We screened 10 potential intronic and3'UTR targeted microRNAs by analysis, shown in Red boxes,and these microRNAs are originated from intronic sequences ofessential cardiac marker genes and these microRNAs are alsotargeted same cardiac marker genes. Previously reported andcharacterized microRNAs are shown in purple boxes, alsofound during our analysis which makes validate our study.
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Figure 2: Possible Role of screened microRNAs in cardiachypertrophy. During cardiac hypertrophy normal heartincreases in size due to re-expression of cardiac marker genes(shown in white boxes). We screened 10 potential intronic and3'UTR targeted microRNAs by analysis, shown in Red boxes,and these microRNAs are originated from intronic sequences ofessential cardiac marker genes and these microRNAs are alsotargeted same cardiac marker genes. Previously reported andcharacterized microRNAs are shown in purple boxes, alsofound during our analysis which makes validate our study.

Mentions: We found 10 true putative regulatory miRNAs after comparative analysis between the intronic miRNAs and 3'UTRtargeted miRNAs using computational tools. hsa-Mir-4710, hsa-Mir-3934, hsa-Mir-665 & has-Mir-4695-5p, hsa-Mir-4476 & hsa-Mir-4763-3p, hsa-Mir-30d, , hsa-Mir-4489 & has-Mir-4638-5p &hsa-Mir-4635 in respect to ANP, BNP, TNNT2, TNNI3, α-Actinin, MYH-7 were identified, as shown in Table 2 (seesupplementary material). These 10 true putative regulatorymicroRNAs are fishing out from the intronic sequences ofselected cardiac marker genes and all these 10 potentialmicroRNAs are targeted the same selected cardiac markergenes. Therefore, these 10 true putative regulatory microRNAscan be hypothesized as potential microRNAs regulating thecardiac development and diseases such as hypertrophy wherere-expression of fetal genes has been witnessed (Figure 2).These uncharacterized microRNAs can further be studied tounderstand the mechanism of their effect on cardiac geneprogram. They may play significant role in regulating thecardiac hypertrophy. After characterizing the role of themiRNAs screened by our insilico study, miRNA mimic andAntimir can be designed for future therapeutics against cardiachypertrophy.


Screening of MicroRNA as potential CardiomiRs in Rattus noveregicus Heart related Dataset.

Rustagi Y, Rani V - Bioinformation (2013)

Possible Role of screened microRNAs in cardiachypertrophy. During cardiac hypertrophy normal heartincreases in size due to re-expression of cardiac marker genes(shown in white boxes). We screened 10 potential intronic and3'UTR targeted microRNAs by analysis, shown in Red boxes,and these microRNAs are originated from intronic sequences ofessential cardiac marker genes and these microRNAs are alsotargeted same cardiac marker genes. Previously reported andcharacterized microRNAs are shown in purple boxes, alsofound during our analysis which makes validate our study.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3842578&req=5

Figure 2: Possible Role of screened microRNAs in cardiachypertrophy. During cardiac hypertrophy normal heartincreases in size due to re-expression of cardiac marker genes(shown in white boxes). We screened 10 potential intronic and3'UTR targeted microRNAs by analysis, shown in Red boxes,and these microRNAs are originated from intronic sequences ofessential cardiac marker genes and these microRNAs are alsotargeted same cardiac marker genes. Previously reported andcharacterized microRNAs are shown in purple boxes, alsofound during our analysis which makes validate our study.
Mentions: We found 10 true putative regulatory miRNAs after comparative analysis between the intronic miRNAs and 3'UTRtargeted miRNAs using computational tools. hsa-Mir-4710, hsa-Mir-3934, hsa-Mir-665 & has-Mir-4695-5p, hsa-Mir-4476 & hsa-Mir-4763-3p, hsa-Mir-30d, , hsa-Mir-4489 & has-Mir-4638-5p &hsa-Mir-4635 in respect to ANP, BNP, TNNT2, TNNI3, α-Actinin, MYH-7 were identified, as shown in Table 2 (seesupplementary material). These 10 true putative regulatorymicroRNAs are fishing out from the intronic sequences ofselected cardiac marker genes and all these 10 potentialmicroRNAs are targeted the same selected cardiac markergenes. Therefore, these 10 true putative regulatory microRNAscan be hypothesized as potential microRNAs regulating thecardiac development and diseases such as hypertrophy wherere-expression of fetal genes has been witnessed (Figure 2).These uncharacterized microRNAs can further be studied tounderstand the mechanism of their effect on cardiac geneprogram. They may play significant role in regulating thecardiac hypertrophy. After characterizing the role of themiRNAs screened by our insilico study, miRNA mimic andAntimir can be designed for future therapeutics against cardiachypertrophy.

Bottom Line: MicroRNAs (miRNAs) are the naturally expressed small, 18~25 nts long non-coding single stranded RNAs, which inhibit the translation by interacting with the 3' untranslated region (UTR) of specific mRNA targets or by repression of posttranscriptional modification of mRNAs.Their role and association with several disease progressions is of interest in recent years.Therefore, we analyzed their features using a dataset (# ≈1400 #) of potential intronic and 3'UTR targeted miRNAs from known cardiac marker genes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, 201307, Uttar Pradesh, India.

ABSTRACT
MicroRNAs (miRNAs) are the naturally expressed small, 18~25 nts long non-coding single stranded RNAs, which inhibit the translation by interacting with the 3' untranslated region (UTR) of specific mRNA targets or by repression of posttranscriptional modification of mRNAs. MiRNAs are found to regulate the differentiation, development, function and stress responsive growth of cardiac cells. Their role and association with several disease progressions is of interest in recent years. Our interest is to study their role in cardiac hypertrophy (characterized by increased cell size, protein synthesis and reactivation of gene pathways). Therefore, we analyzed their features using a dataset (# ≈1400 #) of potential intronic and 3'UTR targeted miRNAs from known cardiac marker genes. We report 10 uncharacterized miRNAs regulating cardiac marker genes during cardiac hypertrophy and other cardiac diseases.

No MeSH data available.


Related in: MedlinePlus