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Anthocyanin-dependent anoxygenic photosynthesis in coloured flower petals?

Lysenko V, Varduny T - Sci Rep (2013)

Bottom Line: We found that the supposed chlorophylless photosynthesis is an anoxygenic, anthocyanin-dependent process occurring in blue flower petals (ADAPFP), accompanied by non-respiratory light-dependent oxygen uptake and a 1.5-fold photoinduced increase in ATP levels.Using a simple, adhesive tape stripping technique, we have obtained a backside image of an intact flower petal epidermis, revealing sword-shaped ingrowths connecting the cell wall and vacuole, which is of interest for the further study of possible vacuole-related photosynthesis.Approaches to the interpretations of ADAPFP are discussed, and we conclude that these results are not impossible in terms of the known photochemistry of anthocyanins.

View Article: PubMed Central - PubMed

Affiliation: Southern Federal University, Rostov-on-Don 344090, Russia.

ABSTRACT
Chlorophylless flower petals are known to be composed of non-photosynthetic tissues. Here, we show that the light energy storage that can be photoacoustically measured in flower petals of Petunia hybrida is approximately 10-12%. We found that the supposed chlorophylless photosynthesis is an anoxygenic, anthocyanin-dependent process occurring in blue flower petals (ADAPFP), accompanied by non-respiratory light-dependent oxygen uptake and a 1.5-fold photoinduced increase in ATP levels. Using a simple, adhesive tape stripping technique, we have obtained a backside image of an intact flower petal epidermis, revealing sword-shaped ingrowths connecting the cell wall and vacuole, which is of interest for the further study of possible vacuole-related photosynthesis. Approaches to the interpretations of ADAPFP are discussed, and we conclude that these results are not impossible in terms of the known photochemistry of anthocyanins.

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Adaxial flower petal epidermis of P. hybrida.Bottom view. The image is created by combining the total of 8 images focused at different depths of the field. It can be seen that ingrowths of the epidermal cell wall are terminated by a widenings closely adjoining the vacuole. See also the 3D-model (Supplementary Data 2).
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f4: Adaxial flower petal epidermis of P. hybrida.Bottom view. The image is created by combining the total of 8 images focused at different depths of the field. It can be seen that ingrowths of the epidermal cell wall are terminated by a widenings closely adjoining the vacuole. See also the 3D-model (Supplementary Data 2).

Mentions: Assuming that the proposed ADAPFP is located in vacuoles or in the vacuolar membrane, the adjoining structures are of special interest. In our work peculiar structures, previously not described in the scientific literature (resembling “jigsaw pieces” or “stitches”) were revealed using simple light microscopy in the P. hybrida petal epidermis as ingrowths from the cell wall toward the centre of cell. However, in this case, a more detailed picture could not be obtained when studying living cells due to the interference of overlying parts of vacuoles and of underlying cells. In the present research the flower petal epidermis of P. hybrida was isolated using a specially developed, simple adhesive-tape stripping technique and was studied by light microscopy using HeliconFocus software allowing for the creation of a single completely focused image and 3D-model from several partially focused images by combining the focused areas. The combined image clearly shows that the ingrowths of the epidermal cell wall are terminated by widenings and are adjoined to vacuoles, much like clavate corpuscles (Fig. 4). As shown using the 3D model compiled in the animated *.gif file (Supplementary Video), these ingrowths are sword-shaped and stretched in height to approximately one half of the vacuole height. The obtained morphological data provide a strong argument for the further investigations of these unusual structures.


Anthocyanin-dependent anoxygenic photosynthesis in coloured flower petals?

Lysenko V, Varduny T - Sci Rep (2013)

Adaxial flower petal epidermis of P. hybrida.Bottom view. The image is created by combining the total of 8 images focused at different depths of the field. It can be seen that ingrowths of the epidermal cell wall are terminated by a widenings closely adjoining the vacuole. See also the 3D-model (Supplementary Data 2).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3842538&req=5

f4: Adaxial flower petal epidermis of P. hybrida.Bottom view. The image is created by combining the total of 8 images focused at different depths of the field. It can be seen that ingrowths of the epidermal cell wall are terminated by a widenings closely adjoining the vacuole. See also the 3D-model (Supplementary Data 2).
Mentions: Assuming that the proposed ADAPFP is located in vacuoles or in the vacuolar membrane, the adjoining structures are of special interest. In our work peculiar structures, previously not described in the scientific literature (resembling “jigsaw pieces” or “stitches”) were revealed using simple light microscopy in the P. hybrida petal epidermis as ingrowths from the cell wall toward the centre of cell. However, in this case, a more detailed picture could not be obtained when studying living cells due to the interference of overlying parts of vacuoles and of underlying cells. In the present research the flower petal epidermis of P. hybrida was isolated using a specially developed, simple adhesive-tape stripping technique and was studied by light microscopy using HeliconFocus software allowing for the creation of a single completely focused image and 3D-model from several partially focused images by combining the focused areas. The combined image clearly shows that the ingrowths of the epidermal cell wall are terminated by widenings and are adjoined to vacuoles, much like clavate corpuscles (Fig. 4). As shown using the 3D model compiled in the animated *.gif file (Supplementary Video), these ingrowths are sword-shaped and stretched in height to approximately one half of the vacuole height. The obtained morphological data provide a strong argument for the further investigations of these unusual structures.

Bottom Line: We found that the supposed chlorophylless photosynthesis is an anoxygenic, anthocyanin-dependent process occurring in blue flower petals (ADAPFP), accompanied by non-respiratory light-dependent oxygen uptake and a 1.5-fold photoinduced increase in ATP levels.Using a simple, adhesive tape stripping technique, we have obtained a backside image of an intact flower petal epidermis, revealing sword-shaped ingrowths connecting the cell wall and vacuole, which is of interest for the further study of possible vacuole-related photosynthesis.Approaches to the interpretations of ADAPFP are discussed, and we conclude that these results are not impossible in terms of the known photochemistry of anthocyanins.

View Article: PubMed Central - PubMed

Affiliation: Southern Federal University, Rostov-on-Don 344090, Russia.

ABSTRACT
Chlorophylless flower petals are known to be composed of non-photosynthetic tissues. Here, we show that the light energy storage that can be photoacoustically measured in flower petals of Petunia hybrida is approximately 10-12%. We found that the supposed chlorophylless photosynthesis is an anoxygenic, anthocyanin-dependent process occurring in blue flower petals (ADAPFP), accompanied by non-respiratory light-dependent oxygen uptake and a 1.5-fold photoinduced increase in ATP levels. Using a simple, adhesive tape stripping technique, we have obtained a backside image of an intact flower petal epidermis, revealing sword-shaped ingrowths connecting the cell wall and vacuole, which is of interest for the further study of possible vacuole-related photosynthesis. Approaches to the interpretations of ADAPFP are discussed, and we conclude that these results are not impossible in terms of the known photochemistry of anthocyanins.

Show MeSH