Limits...
Anthocyanin-dependent anoxygenic photosynthesis in coloured flower petals?

Lysenko V, Varduny T - Sci Rep (2013)

Bottom Line: We found that the supposed chlorophylless photosynthesis is an anoxygenic, anthocyanin-dependent process occurring in blue flower petals (ADAPFP), accompanied by non-respiratory light-dependent oxygen uptake and a 1.5-fold photoinduced increase in ATP levels.Using a simple, adhesive tape stripping technique, we have obtained a backside image of an intact flower petal epidermis, revealing sword-shaped ingrowths connecting the cell wall and vacuole, which is of interest for the further study of possible vacuole-related photosynthesis.Approaches to the interpretations of ADAPFP are discussed, and we conclude that these results are not impossible in terms of the known photochemistry of anthocyanins.

View Article: PubMed Central - PubMed

Affiliation: Southern Federal University, Rostov-on-Don 344090, Russia.

ABSTRACT
Chlorophylless flower petals are known to be composed of non-photosynthetic tissues. Here, we show that the light energy storage that can be photoacoustically measured in flower petals of Petunia hybrida is approximately 10-12%. We found that the supposed chlorophylless photosynthesis is an anoxygenic, anthocyanin-dependent process occurring in blue flower petals (ADAPFP), accompanied by non-respiratory light-dependent oxygen uptake and a 1.5-fold photoinduced increase in ATP levels. Using a simple, adhesive tape stripping technique, we have obtained a backside image of an intact flower petal epidermis, revealing sword-shaped ingrowths connecting the cell wall and vacuole, which is of interest for the further study of possible vacuole-related photosynthesis. Approaches to the interpretations of ADAPFP are discussed, and we conclude that these results are not impossible in terms of the known photochemistry of anthocyanins.

Show MeSH
ATP and ADP levels in the blue (a) and white (b) P. hybrida flower petals after 30 min of dark adaptation or after 30- 60- and 180-s strong (900 μmol photons m−2 s−1, 2700 K°) light switching on.Error bars = SD; n = 8.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3842538&req=5

f3: ATP and ADP levels in the blue (a) and white (b) P. hybrida flower petals after 30 min of dark adaptation or after 30- 60- and 180-s strong (900 μmol photons m−2 s−1, 2700 K°) light switching on.Error bars = SD; n = 8.

Mentions: The ATP level in blue P. hybrida flower petal epidermis increased 1.5 times when measured after 30 s of strong light illumination (Fig. 3a), whereas the concurent ADP level decreased 1.3 times. The ATP level subsequently measured at 60 and 180 seconds of the illumination, and showed a weak decrease. Similar ATP kinetics were observed in experiments on the light excitation of chlorophyll photosynthesis in the green algae Ankistrodesmus braunii and chloroplasts from Pisum sativum, where the local maximum at 20–30 s was followed by a weak decrease in the ATP level16. The corresponding ATP/ADP ratio changes in the petal tissues were more strongly pronounced. In contrast to that of blue flower petals, the epidermis of white flower petals did not respond to strong continuous light switching on (Fig. 3b), clearly resulting from that the anthocyanins are necessary here for the development of the photodependent changes of phosphorylation.


Anthocyanin-dependent anoxygenic photosynthesis in coloured flower petals?

Lysenko V, Varduny T - Sci Rep (2013)

ATP and ADP levels in the blue (a) and white (b) P. hybrida flower petals after 30 min of dark adaptation or after 30- 60- and 180-s strong (900 μmol photons m−2 s−1, 2700 K°) light switching on.Error bars = SD; n = 8.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3842538&req=5

f3: ATP and ADP levels in the blue (a) and white (b) P. hybrida flower petals after 30 min of dark adaptation or after 30- 60- and 180-s strong (900 μmol photons m−2 s−1, 2700 K°) light switching on.Error bars = SD; n = 8.
Mentions: The ATP level in blue P. hybrida flower petal epidermis increased 1.5 times when measured after 30 s of strong light illumination (Fig. 3a), whereas the concurent ADP level decreased 1.3 times. The ATP level subsequently measured at 60 and 180 seconds of the illumination, and showed a weak decrease. Similar ATP kinetics were observed in experiments on the light excitation of chlorophyll photosynthesis in the green algae Ankistrodesmus braunii and chloroplasts from Pisum sativum, where the local maximum at 20–30 s was followed by a weak decrease in the ATP level16. The corresponding ATP/ADP ratio changes in the petal tissues were more strongly pronounced. In contrast to that of blue flower petals, the epidermis of white flower petals did not respond to strong continuous light switching on (Fig. 3b), clearly resulting from that the anthocyanins are necessary here for the development of the photodependent changes of phosphorylation.

Bottom Line: We found that the supposed chlorophylless photosynthesis is an anoxygenic, anthocyanin-dependent process occurring in blue flower petals (ADAPFP), accompanied by non-respiratory light-dependent oxygen uptake and a 1.5-fold photoinduced increase in ATP levels.Using a simple, adhesive tape stripping technique, we have obtained a backside image of an intact flower petal epidermis, revealing sword-shaped ingrowths connecting the cell wall and vacuole, which is of interest for the further study of possible vacuole-related photosynthesis.Approaches to the interpretations of ADAPFP are discussed, and we conclude that these results are not impossible in terms of the known photochemistry of anthocyanins.

View Article: PubMed Central - PubMed

Affiliation: Southern Federal University, Rostov-on-Don 344090, Russia.

ABSTRACT
Chlorophylless flower petals are known to be composed of non-photosynthetic tissues. Here, we show that the light energy storage that can be photoacoustically measured in flower petals of Petunia hybrida is approximately 10-12%. We found that the supposed chlorophylless photosynthesis is an anoxygenic, anthocyanin-dependent process occurring in blue flower petals (ADAPFP), accompanied by non-respiratory light-dependent oxygen uptake and a 1.5-fold photoinduced increase in ATP levels. Using a simple, adhesive tape stripping technique, we have obtained a backside image of an intact flower petal epidermis, revealing sword-shaped ingrowths connecting the cell wall and vacuole, which is of interest for the further study of possible vacuole-related photosynthesis. Approaches to the interpretations of ADAPFP are discussed, and we conclude that these results are not impossible in terms of the known photochemistry of anthocyanins.

Show MeSH