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The Relationship between Intestinal Motility and Interstitial Cells of Cajal in Nonalcoholic Fatty Liver Mice.

Lu ML, Huang H, Liu LM, Chang J - Hepat Mon (2013)

Bottom Line: However, NAFLD shows an increasing trend in China every year, which has attracted the attention of national health authorities.The previous studies have shown that NAFLD caused severe gastrointestinal motor disorders, but little is known about the interstitial cells of Cajal (ICC) role in gastrointestinal motor disorders.The hepatic tissue slices were used for pathological observation.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.

ABSTRACT

Background: Nonalcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease in western world. However, NAFLD shows an increasing trend in China every year, which has attracted the attention of national health authorities. The previous studies have shown that NAFLD caused severe gastrointestinal motor disorders, but little is known about the interstitial cells of Cajal (ICC) role in gastrointestinal motor disorders.

Objectives: The aim of this study was to observe the ICC in jejunum of nonalcoholic fatty liver mice by immunohistochemistry and assessed the relationship between intestinal motility and ICC.

Materials and methods: Thirty five Sprague-Dawley (SD) rats were randomly divided into nonalcoholic fatty liver (n = 25) and control groups (n = 10), rats were housed individually in cages and had free access to food and water, nonalcoholic fatty liver group was duplicated by high-fat diet (consisted of ordinary food, 20 g/kg cholesterol and 100 g/kg fat) feeding. Dextran blue-2000 was used to monitor the intestinal motility. The proximal small intestine was harvested to investigate the C-kit positive ICC. The hepatic tissue slices were used for pathological observation.

Results: Nonalcoholic fatty liver disease was successfully established. The intestinal motility in nonalcoholic fatty liver group (49.5 ± 10.9) was weaker compared to the control group (57.3 ± 8.9), P < 0.05. The rate of ICC also have shown statistically significant differences between nonalcoholic fatty liver (4.87 ± 2.97/mm (2)) and control groups (6.54 ± 3.13/mm (2)), P < 0.05.

Conclusions: ICC may be related to the intestinal motility in nonalcoholic fatty liver mice.

No MeSH data available.


Related in: MedlinePlus

Pathological Changes of Liver After 12 weeks of High Fat DietHepatocyte ballooning, mixed lymphocytic and neutrophilic inflammatory infiltrate in perivenular areas, HE × 200
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fig6356: Pathological Changes of Liver After 12 weeks of High Fat DietHepatocyte ballooning, mixed lymphocytic and neutrophilic inflammatory infiltrate in perivenular areas, HE × 200

Mentions: The pathologically nonalcoholic fatty liver mode has been successfully established (Figure 1). The intestinal motility of nonalcoholic fatty liver group (49.5 ± 10.9) weakened compared to control group (57.3 ± 8.9), P < 0.05 (Figure 2). Brown ICC membrane was recognized as a positive result. ICC mainly located in the circular and longitudinal muscle layers. C-kit positive ICC also have shown statistically significant differences between nonalcoholic fatty liver (4.87 ± 2.97/mm 2) and control groups (6.54 ± 3.13/mm 2 ), P < 0.05 (Figure 3, Figure 4).


The Relationship between Intestinal Motility and Interstitial Cells of Cajal in Nonalcoholic Fatty Liver Mice.

Lu ML, Huang H, Liu LM, Chang J - Hepat Mon (2013)

Pathological Changes of Liver After 12 weeks of High Fat DietHepatocyte ballooning, mixed lymphocytic and neutrophilic inflammatory infiltrate in perivenular areas, HE × 200
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3842523&req=5

fig6356: Pathological Changes of Liver After 12 weeks of High Fat DietHepatocyte ballooning, mixed lymphocytic and neutrophilic inflammatory infiltrate in perivenular areas, HE × 200
Mentions: The pathologically nonalcoholic fatty liver mode has been successfully established (Figure 1). The intestinal motility of nonalcoholic fatty liver group (49.5 ± 10.9) weakened compared to control group (57.3 ± 8.9), P < 0.05 (Figure 2). Brown ICC membrane was recognized as a positive result. ICC mainly located in the circular and longitudinal muscle layers. C-kit positive ICC also have shown statistically significant differences between nonalcoholic fatty liver (4.87 ± 2.97/mm 2) and control groups (6.54 ± 3.13/mm 2 ), P < 0.05 (Figure 3, Figure 4).

Bottom Line: However, NAFLD shows an increasing trend in China every year, which has attracted the attention of national health authorities.The previous studies have shown that NAFLD caused severe gastrointestinal motor disorders, but little is known about the interstitial cells of Cajal (ICC) role in gastrointestinal motor disorders.The hepatic tissue slices were used for pathological observation.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.

ABSTRACT

Background: Nonalcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease in western world. However, NAFLD shows an increasing trend in China every year, which has attracted the attention of national health authorities. The previous studies have shown that NAFLD caused severe gastrointestinal motor disorders, but little is known about the interstitial cells of Cajal (ICC) role in gastrointestinal motor disorders.

Objectives: The aim of this study was to observe the ICC in jejunum of nonalcoholic fatty liver mice by immunohistochemistry and assessed the relationship between intestinal motility and ICC.

Materials and methods: Thirty five Sprague-Dawley (SD) rats were randomly divided into nonalcoholic fatty liver (n = 25) and control groups (n = 10), rats were housed individually in cages and had free access to food and water, nonalcoholic fatty liver group was duplicated by high-fat diet (consisted of ordinary food, 20 g/kg cholesterol and 100 g/kg fat) feeding. Dextran blue-2000 was used to monitor the intestinal motility. The proximal small intestine was harvested to investigate the C-kit positive ICC. The hepatic tissue slices were used for pathological observation.

Results: Nonalcoholic fatty liver disease was successfully established. The intestinal motility in nonalcoholic fatty liver group (49.5 ± 10.9) was weaker compared to the control group (57.3 ± 8.9), P < 0.05. The rate of ICC also have shown statistically significant differences between nonalcoholic fatty liver (4.87 ± 2.97/mm (2)) and control groups (6.54 ± 3.13/mm (2)), P < 0.05.

Conclusions: ICC may be related to the intestinal motility in nonalcoholic fatty liver mice.

No MeSH data available.


Related in: MedlinePlus