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Effects of Nano-sized Carbon Black on the Lungs of High Fat-diet Induced Overweight Rats.

Lim CH, Kang M, Han JH, Yun HI - Environ Health Toxicol (2013)

Bottom Line: This study was conducted to determine whether nano-sized carbon black exposure results in greater damage in high fat diet-induced overweight rats than normal weight ones and to identify the possible causes of any differences.Differential cell counts, lactate dehydrogenase (LDH) activities and albumin concentrations were measured in bronchoalveolar lavage (BAL) fluid, and histopathological findings in the lungs were evaluated.Tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 were measured in BAL fluid and supernatants of lipopolysaccharide(LPS)-stimulated lymphocyte culture.

View Article: PubMed Central - PubMed

Affiliation: Center for Chemicals Safety and Health Research, Occupational Safety and Health Research Institute, KOSHA, Daejeon, Korea.

ABSTRACT

Objectives: This study was conducted to determine whether nano-sized carbon black exposure results in greater damage in high fat diet-induced overweight rats than normal weight ones and to identify the possible causes of any differences.

Methods: Two groups of Sprague-Dawley rats allocated by body weight (normal and overweight) were exposed to aerosolized nano-sized carbon black for 6 hours a day, 5 days per week over a 4-week period. Differential cell counts, lactate dehydrogenase (LDH) activities and albumin concentrations were measured in bronchoalveolar lavage (BAL) fluid, and histopathological findings in the lungs were evaluated. Tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 were measured in BAL fluid and supernatants of lipopolysaccharide(LPS)-stimulated lymphocyte culture.

Results: Rats exposed to high concentrations of nano-sized carbon black showed significantly increased (p<0.05) polymorphonuclear leukocyte number and LDH activity in the BAL fluid from both overweight and normal rats. Mild histopathological changes were observed in normal rats irrespective of carbon black concentrations. However, severe histological scores were found in overweight rats (1.75±0.46, 2.25±0.46, and 2.88±0.35 after low, medium, and high concentration exposures). Proinflammatory cytokine levels of TNF-α and IL-6 were significantly higher in the supernatant of LPS-stimulated lymphocytes of overweight rats, whereas there was no significant difference in the BAL fluid between normal and overweight rats.

Conclusions: Inflammation and damage to lungs exposed to nano-sized carbon black was more severe in high fat diet-induced overweight rats compared to normal rats.

No MeSH data available.


Related in: MedlinePlus

Tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 levels in the bronchoalveolar lavage (BAL) fluid and supernatant of lipopolysaccharide (LPS)-stimulated lymphocyte culture. (A) TNF-α in the BAL fluid. (B) IL-6 in the BAL fluid. (C) TNF-α in the supernatant of the LPS-stimulated lymphocyte culture. (D) IL-6 in the supernatant of the LPS-stimulated lymphocyte culture. Error bars indicate the standard error of the mean. *p<0.05 vs. control; **p<0.01 vs. control; †p<0.05 vs. normal-weight; ‡p<0.01 vs. normal-weight (n=8).
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Figure 6: Tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 levels in the bronchoalveolar lavage (BAL) fluid and supernatant of lipopolysaccharide (LPS)-stimulated lymphocyte culture. (A) TNF-α in the BAL fluid. (B) IL-6 in the BAL fluid. (C) TNF-α in the supernatant of the LPS-stimulated lymphocyte culture. (D) IL-6 in the supernatant of the LPS-stimulated lymphocyte culture. Error bars indicate the standard error of the mean. *p<0.05 vs. control; **p<0.01 vs. control; †p<0.05 vs. normal-weight; ‡p<0.01 vs. normal-weight (n=8).

Mentions: TNF-α levels in the BAL fluid only increased in overweight rats exposed to medium and high concentrations of carbon black (Figure 6A), while IL-6 levels stayed constant regardless of carbon black exposure both in normal- and over-weight rats (Figure 6B). In contrast, TNF-α and IL-6 levels in the supernatant of LPS-stimulated auxiliary lymphocyte cultures taken from overweight rats were significantly increased by exposure to medium and high concentrations of carbon black (Figure 6C and 6D).


Effects of Nano-sized Carbon Black on the Lungs of High Fat-diet Induced Overweight Rats.

Lim CH, Kang M, Han JH, Yun HI - Environ Health Toxicol (2013)

Tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 levels in the bronchoalveolar lavage (BAL) fluid and supernatant of lipopolysaccharide (LPS)-stimulated lymphocyte culture. (A) TNF-α in the BAL fluid. (B) IL-6 in the BAL fluid. (C) TNF-α in the supernatant of the LPS-stimulated lymphocyte culture. (D) IL-6 in the supernatant of the LPS-stimulated lymphocyte culture. Error bars indicate the standard error of the mean. *p<0.05 vs. control; **p<0.01 vs. control; †p<0.05 vs. normal-weight; ‡p<0.01 vs. normal-weight (n=8).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3842451&req=5

Figure 6: Tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 levels in the bronchoalveolar lavage (BAL) fluid and supernatant of lipopolysaccharide (LPS)-stimulated lymphocyte culture. (A) TNF-α in the BAL fluid. (B) IL-6 in the BAL fluid. (C) TNF-α in the supernatant of the LPS-stimulated lymphocyte culture. (D) IL-6 in the supernatant of the LPS-stimulated lymphocyte culture. Error bars indicate the standard error of the mean. *p<0.05 vs. control; **p<0.01 vs. control; †p<0.05 vs. normal-weight; ‡p<0.01 vs. normal-weight (n=8).
Mentions: TNF-α levels in the BAL fluid only increased in overweight rats exposed to medium and high concentrations of carbon black (Figure 6A), while IL-6 levels stayed constant regardless of carbon black exposure both in normal- and over-weight rats (Figure 6B). In contrast, TNF-α and IL-6 levels in the supernatant of LPS-stimulated auxiliary lymphocyte cultures taken from overweight rats were significantly increased by exposure to medium and high concentrations of carbon black (Figure 6C and 6D).

Bottom Line: This study was conducted to determine whether nano-sized carbon black exposure results in greater damage in high fat diet-induced overweight rats than normal weight ones and to identify the possible causes of any differences.Differential cell counts, lactate dehydrogenase (LDH) activities and albumin concentrations were measured in bronchoalveolar lavage (BAL) fluid, and histopathological findings in the lungs were evaluated.Tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 were measured in BAL fluid and supernatants of lipopolysaccharide(LPS)-stimulated lymphocyte culture.

View Article: PubMed Central - PubMed

Affiliation: Center for Chemicals Safety and Health Research, Occupational Safety and Health Research Institute, KOSHA, Daejeon, Korea.

ABSTRACT

Objectives: This study was conducted to determine whether nano-sized carbon black exposure results in greater damage in high fat diet-induced overweight rats than normal weight ones and to identify the possible causes of any differences.

Methods: Two groups of Sprague-Dawley rats allocated by body weight (normal and overweight) were exposed to aerosolized nano-sized carbon black for 6 hours a day, 5 days per week over a 4-week period. Differential cell counts, lactate dehydrogenase (LDH) activities and albumin concentrations were measured in bronchoalveolar lavage (BAL) fluid, and histopathological findings in the lungs were evaluated. Tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 were measured in BAL fluid and supernatants of lipopolysaccharide(LPS)-stimulated lymphocyte culture.

Results: Rats exposed to high concentrations of nano-sized carbon black showed significantly increased (p<0.05) polymorphonuclear leukocyte number and LDH activity in the BAL fluid from both overweight and normal rats. Mild histopathological changes were observed in normal rats irrespective of carbon black concentrations. However, severe histological scores were found in overweight rats (1.75±0.46, 2.25±0.46, and 2.88±0.35 after low, medium, and high concentration exposures). Proinflammatory cytokine levels of TNF-α and IL-6 were significantly higher in the supernatant of LPS-stimulated lymphocytes of overweight rats, whereas there was no significant difference in the BAL fluid between normal and overweight rats.

Conclusions: Inflammation and damage to lungs exposed to nano-sized carbon black was more severe in high fat diet-induced overweight rats compared to normal rats.

No MeSH data available.


Related in: MedlinePlus