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Characterization of a small auxin-up RNA (SAUR)-like gene involved in Arabidopsis thaliana development.

Markakis MN, Boron AK, Van Loock B, Saini K, Cirera S, Verbelen JP, Vissenberg K - PLoS ONE (2013)

Bottom Line: From a micro-array performed on roots that were treated with aminocyclopropane-1-carboxylic acid (ACC), the precursor of ethylene, a Small auxin-up RNA (SAUR)-like gene was found to be up regulated.Furthermore, confocal analysis of protein-GFP fusions localized the protein in the nucleus, cytoplasm and plasma membrane.SAUR76 expression was quantified in several mutants in ethylene and auxin-related pathways, which led to the conclusion that the expression of SAUR76 is mainly regulated by the increase in auxin that results from the addition of ACC, rather than by ACC itself.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, University of Antwerp, Antwerpen, Belgium.

ABSTRACT
The root of Arabidopsis thaliana is used as a model system to unravel the molecular nature of cell elongation and its arrest. From a micro-array performed on roots that were treated with aminocyclopropane-1-carboxylic acid (ACC), the precursor of ethylene, a Small auxin-up RNA (SAUR)-like gene was found to be up regulated. As it appeared as the 76th gene in the family, it was named SAUR76. Root and leaf growth of overexpression lines ectopically expressing SAUR76 indicated the possible involvement of the gene in the division process. Using promoter::GUS and GFP lines strong expression was seen in endodermal and pericycle cells at the end of the elongation zone and during several stages of lateral root primordia development. ACC and IAA/NAA were able to induce a strong up regulation of the gene and changed the expression towards cortical and even epidermal cells at the beginning of the elongation zone. Confirmation of this up regulation of expression was delivered using qPCR, which also indicated that the expression quickly returned to normal levels when the inducing IAA-stimulus was removed, a behaviour also seen in other SAUR genes. Furthermore, confocal analysis of protein-GFP fusions localized the protein in the nucleus, cytoplasm and plasma membrane. SAUR76 expression was quantified in several mutants in ethylene and auxin-related pathways, which led to the conclusion that the expression of SAUR76 is mainly regulated by the increase in auxin that results from the addition of ACC, rather than by ACC itself.

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Schematical representation of the SAUR76 gene and its relationship to other members of the SAUR-family.A) Linear representation of the SAUR76 gene, including the insertion position of the T-DNA in the knock-out line. Red: UTR; orange: exon; ATG: start codon; TAA: stop codon. B) Phylogenetic tree of the Arabidopsis SAUR-family including SAUR-like proteins.
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pone-0082596-g001: Schematical representation of the SAUR76 gene and its relationship to other members of the SAUR-family.A) Linear representation of the SAUR76 gene, including the insertion position of the T-DNA in the knock-out line. Red: UTR; orange: exon; ATG: start codon; TAA: stop codon. B) Phylogenetic tree of the Arabidopsis SAUR-family including SAUR-like proteins.

Mentions: To decipher the mechanism(s) by which ACC mediates the control of cell elongation, a microarray was performed on control and 3h ACC-treated roots. This transcriptome analysis identified 240 differentially expressed genes [26]. Besides genes with a presumptive role in the ethylene-signalling pathway and cell wall metabolism, also several auxin-related genes were identified. Since ethylene is documented to influence auxin transport and biosynthesis [27–30] as part of well documented ethylene/auxin crosstalk [31], this was as expected. In the list of the 10 most upregulated genes by ACC, one predicted auxin-related gene, At5g20820, was present. This gene encodes a one-exon-protein of 127 amino acids (Figure 1A) that is, according to the TAIR website (www.arabidopsis.org), predicted to belong to the SAUR-like auxin-responsive protein family based on the presence of an auxin-responsive SAUR-protein domain (IPR003676).


Characterization of a small auxin-up RNA (SAUR)-like gene involved in Arabidopsis thaliana development.

Markakis MN, Boron AK, Van Loock B, Saini K, Cirera S, Verbelen JP, Vissenberg K - PLoS ONE (2013)

Schematical representation of the SAUR76 gene and its relationship to other members of the SAUR-family.A) Linear representation of the SAUR76 gene, including the insertion position of the T-DNA in the knock-out line. Red: UTR; orange: exon; ATG: start codon; TAA: stop codon. B) Phylogenetic tree of the Arabidopsis SAUR-family including SAUR-like proteins.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3842426&req=5

pone-0082596-g001: Schematical representation of the SAUR76 gene and its relationship to other members of the SAUR-family.A) Linear representation of the SAUR76 gene, including the insertion position of the T-DNA in the knock-out line. Red: UTR; orange: exon; ATG: start codon; TAA: stop codon. B) Phylogenetic tree of the Arabidopsis SAUR-family including SAUR-like proteins.
Mentions: To decipher the mechanism(s) by which ACC mediates the control of cell elongation, a microarray was performed on control and 3h ACC-treated roots. This transcriptome analysis identified 240 differentially expressed genes [26]. Besides genes with a presumptive role in the ethylene-signalling pathway and cell wall metabolism, also several auxin-related genes were identified. Since ethylene is documented to influence auxin transport and biosynthesis [27–30] as part of well documented ethylene/auxin crosstalk [31], this was as expected. In the list of the 10 most upregulated genes by ACC, one predicted auxin-related gene, At5g20820, was present. This gene encodes a one-exon-protein of 127 amino acids (Figure 1A) that is, according to the TAIR website (www.arabidopsis.org), predicted to belong to the SAUR-like auxin-responsive protein family based on the presence of an auxin-responsive SAUR-protein domain (IPR003676).

Bottom Line: From a micro-array performed on roots that were treated with aminocyclopropane-1-carboxylic acid (ACC), the precursor of ethylene, a Small auxin-up RNA (SAUR)-like gene was found to be up regulated.Furthermore, confocal analysis of protein-GFP fusions localized the protein in the nucleus, cytoplasm and plasma membrane.SAUR76 expression was quantified in several mutants in ethylene and auxin-related pathways, which led to the conclusion that the expression of SAUR76 is mainly regulated by the increase in auxin that results from the addition of ACC, rather than by ACC itself.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, University of Antwerp, Antwerpen, Belgium.

ABSTRACT
The root of Arabidopsis thaliana is used as a model system to unravel the molecular nature of cell elongation and its arrest. From a micro-array performed on roots that were treated with aminocyclopropane-1-carboxylic acid (ACC), the precursor of ethylene, a Small auxin-up RNA (SAUR)-like gene was found to be up regulated. As it appeared as the 76th gene in the family, it was named SAUR76. Root and leaf growth of overexpression lines ectopically expressing SAUR76 indicated the possible involvement of the gene in the division process. Using promoter::GUS and GFP lines strong expression was seen in endodermal and pericycle cells at the end of the elongation zone and during several stages of lateral root primordia development. ACC and IAA/NAA were able to induce a strong up regulation of the gene and changed the expression towards cortical and even epidermal cells at the beginning of the elongation zone. Confirmation of this up regulation of expression was delivered using qPCR, which also indicated that the expression quickly returned to normal levels when the inducing IAA-stimulus was removed, a behaviour also seen in other SAUR genes. Furthermore, confocal analysis of protein-GFP fusions localized the protein in the nucleus, cytoplasm and plasma membrane. SAUR76 expression was quantified in several mutants in ethylene and auxin-related pathways, which led to the conclusion that the expression of SAUR76 is mainly regulated by the increase in auxin that results from the addition of ACC, rather than by ACC itself.

Show MeSH