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Evaluation of the potential effect of transgenic rice expressing Cry1Ab on the hematology and enzyme activity in organs of female Swiss rats.

Wang Y, Wei B, Tian Y, Wang Z, Tian Y, Tan S, Dong S, Song Q - PLoS ONE (2013)

Bottom Line: Similarly, Cry1Ab rice had no effect on enzyme activities in a variety of organs of Swiss rat.Quantitative real-time PCR (qPCR) analysis of the 6 genes encoding enzymes responsible for the major detoxification functions of liver revealed that Cry1Ab rice exerted no influences on the levels of these transcripts in liver of Swiss rat, indicating that significant differences registered in part of the blood biochemical parameters in the 30 day study might result from other untested organs or tissues in response to the stress of exogenous Cry1Ab protein.The results suggest that Cry1Ab protein has no significant long-term (90 day) effects on female Swiss rat.

View Article: PubMed Central - PubMed

Affiliation: College of Bioscience and Biotechnology, Hunan Agriculture University, Changsha, China.

ABSTRACT
To assess the safety of transgenic rice expressing Cry1Ab protein to vertebrates, the effect of Cry1Ab rice on broad health indicators in blood and various organs of Swiss rats were analyzed. The 30 and 90 day safety studies of Cry1Ab rice on female Swiss rats revealed that Cry1Ab rice had no significant effect on the several elements of blood lymph including hemogram, calcium ion concentration and apoptosis rate of lymphocytes, indicating that Cry1Ab protein could not affect the blood lymph of Swiss rat. Similarly, Cry1Ab rice had no effect on enzyme activities in a variety of organs of Swiss rat. However, Cry1Ab rice did have significant effects on the blood biochemistry indexes including urea, triglyceride (TG), glutamic oxalacetic transaminase (AST) and alkaline phosphatase (ALP) after the rats were fed with Cry1Ab rice for 30 days, but not after 90 days, indicating that Cry1Ab protein may influence blood metabolism for a short duration. Quantitative real-time PCR (qPCR) analysis of the 6 genes encoding enzymes responsible for the major detoxification functions of liver revealed that Cry1Ab rice exerted no influences on the levels of these transcripts in liver of Swiss rat, indicating that significant differences registered in part of the blood biochemical parameters in the 30 day study might result from other untested organs or tissues in response to the stress of exogenous Cry1Ab protein. The results suggest that Cry1Ab protein has no significant long-term (90 day) effects on female Swiss rat.

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The variation of apoptosis rate in Swiss rats under the effect of Cry1Ab protein.A1: the 30 day feeding Swiss rats in control group. A2: the 90 day feeding Swiss rats in control group. A3: the 30 day feeding Swiss rats in test group. A4: the 90 days feeding Swiss rats in test group. Note: The X coordinate means the percent of dyeing Annexin V cells. The Y coordinate means the percent of dyeing PI cells. The B1 coordinate means the percent of necrosis cells and the late apoptosis cells. The B2 coordinate means the percent of dead cells of mechanical damage. The B3 coordinate means the percent of survived cells. The B4 coordinate means the percent of the early apoptosis cells.
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pone-0080424-g001: The variation of apoptosis rate in Swiss rats under the effect of Cry1Ab protein.A1: the 30 day feeding Swiss rats in control group. A2: the 90 day feeding Swiss rats in control group. A3: the 30 day feeding Swiss rats in test group. A4: the 90 days feeding Swiss rats in test group. Note: The X coordinate means the percent of dyeing Annexin V cells. The Y coordinate means the percent of dyeing PI cells. The B1 coordinate means the percent of necrosis cells and the late apoptosis cells. The B2 coordinate means the percent of dead cells of mechanical damage. The B3 coordinate means the percent of survived cells. The B4 coordinate means the percent of the early apoptosis cells.

Mentions: Cry1Ab protein had no significant effects on the apoptosis rate of blood lymphocytes of Swiss rat in both 30 and 90 day studies (Fig. 1). The number of apoptosis cells in the 30 days study attained near 0%. Although in the 90 days study, the number of apoptosis cells was 0.1% in the control group and 0.4% in the test group, the differences between them had not attained the significant level.


Evaluation of the potential effect of transgenic rice expressing Cry1Ab on the hematology and enzyme activity in organs of female Swiss rats.

Wang Y, Wei B, Tian Y, Wang Z, Tian Y, Tan S, Dong S, Song Q - PLoS ONE (2013)

The variation of apoptosis rate in Swiss rats under the effect of Cry1Ab protein.A1: the 30 day feeding Swiss rats in control group. A2: the 90 day feeding Swiss rats in control group. A3: the 30 day feeding Swiss rats in test group. A4: the 90 days feeding Swiss rats in test group. Note: The X coordinate means the percent of dyeing Annexin V cells. The Y coordinate means the percent of dyeing PI cells. The B1 coordinate means the percent of necrosis cells and the late apoptosis cells. The B2 coordinate means the percent of dead cells of mechanical damage. The B3 coordinate means the percent of survived cells. The B4 coordinate means the percent of the early apoptosis cells.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3842383&req=5

pone-0080424-g001: The variation of apoptosis rate in Swiss rats under the effect of Cry1Ab protein.A1: the 30 day feeding Swiss rats in control group. A2: the 90 day feeding Swiss rats in control group. A3: the 30 day feeding Swiss rats in test group. A4: the 90 days feeding Swiss rats in test group. Note: The X coordinate means the percent of dyeing Annexin V cells. The Y coordinate means the percent of dyeing PI cells. The B1 coordinate means the percent of necrosis cells and the late apoptosis cells. The B2 coordinate means the percent of dead cells of mechanical damage. The B3 coordinate means the percent of survived cells. The B4 coordinate means the percent of the early apoptosis cells.
Mentions: Cry1Ab protein had no significant effects on the apoptosis rate of blood lymphocytes of Swiss rat in both 30 and 90 day studies (Fig. 1). The number of apoptosis cells in the 30 days study attained near 0%. Although in the 90 days study, the number of apoptosis cells was 0.1% in the control group and 0.4% in the test group, the differences between them had not attained the significant level.

Bottom Line: Similarly, Cry1Ab rice had no effect on enzyme activities in a variety of organs of Swiss rat.Quantitative real-time PCR (qPCR) analysis of the 6 genes encoding enzymes responsible for the major detoxification functions of liver revealed that Cry1Ab rice exerted no influences on the levels of these transcripts in liver of Swiss rat, indicating that significant differences registered in part of the blood biochemical parameters in the 30 day study might result from other untested organs or tissues in response to the stress of exogenous Cry1Ab protein.The results suggest that Cry1Ab protein has no significant long-term (90 day) effects on female Swiss rat.

View Article: PubMed Central - PubMed

Affiliation: College of Bioscience and Biotechnology, Hunan Agriculture University, Changsha, China.

ABSTRACT
To assess the safety of transgenic rice expressing Cry1Ab protein to vertebrates, the effect of Cry1Ab rice on broad health indicators in blood and various organs of Swiss rats were analyzed. The 30 and 90 day safety studies of Cry1Ab rice on female Swiss rats revealed that Cry1Ab rice had no significant effect on the several elements of blood lymph including hemogram, calcium ion concentration and apoptosis rate of lymphocytes, indicating that Cry1Ab protein could not affect the blood lymph of Swiss rat. Similarly, Cry1Ab rice had no effect on enzyme activities in a variety of organs of Swiss rat. However, Cry1Ab rice did have significant effects on the blood biochemistry indexes including urea, triglyceride (TG), glutamic oxalacetic transaminase (AST) and alkaline phosphatase (ALP) after the rats were fed with Cry1Ab rice for 30 days, but not after 90 days, indicating that Cry1Ab protein may influence blood metabolism for a short duration. Quantitative real-time PCR (qPCR) analysis of the 6 genes encoding enzymes responsible for the major detoxification functions of liver revealed that Cry1Ab rice exerted no influences on the levels of these transcripts in liver of Swiss rat, indicating that significant differences registered in part of the blood biochemical parameters in the 30 day study might result from other untested organs or tissues in response to the stress of exogenous Cry1Ab protein. The results suggest that Cry1Ab protein has no significant long-term (90 day) effects on female Swiss rat.

Show MeSH
Related in: MedlinePlus