Detection of CD133 (prominin-1) in a human hepatoblastoma cell line (HuH-6 clone 5).
Bottom Line: An antibody against CD133 decreased cell migration.Methyl-β-cyclodextrin treatment decreased cell adhesion as well as lamellipodium and filopodium formation.The results suggest that CD133 membrane localization plays a role in tumor cell adhesion and migration.
Affiliation: Division of Morphological Science, Biomedical Research Center, Saitama Medical University, Iruma-gun, Saitama, Japan. email@example.comShow MeSH
Related in: MedlinePlus
Mentions: As shown in Figure 9, cell migration decreased after treatment with a CD133 antibody (10 μg/mL) for 24 h. The distance of cell migration from the leading edge was significantly shortened by the CD133 antibody treatment (control group, 187.6 ± 16.0 μm; CD133 treatment group, 96.9 ± 8.2 μm, P < 0.01). However, the cell migration distance was not significantly affected at the lower antibody concentration (1 μg/mL).
Affiliation: Division of Morphological Science, Biomedical Research Center, Saitama Medical University, Iruma-gun, Saitama, Japan. firstname.lastname@example.org