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Detection of CD133 (prominin-1) in a human hepatoblastoma cell line (HuH-6 clone 5).

Akita M, Tanaka K, Murai N, Matsumoto S, Fujita K, Takaki T, Nishiyama H - Microsc. Res. Tech. (2013)

Bottom Line: An antibody against CD133 decreased cell migration.Methyl-β-cyclodextrin treatment decreased cell adhesion as well as lamellipodium and filopodium formation.The results suggest that CD133 membrane localization plays a role in tumor cell adhesion and migration.

View Article: PubMed Central - PubMed

Affiliation: Division of Morphological Science, Biomedical Research Center, Saitama Medical University, Iruma-gun, Saitama, Japan. makita@saitama-med.ac.jp

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Related in: MedlinePlus

Inhibitory effect of CD133 antibody on cell migration. A migration assay was performed using the scratch wound healing assay and cells were viewed under an inverted optical microscope. a: Control. b: CD133 antibody treatment (10 μg/mL). Black lines with arrows at both ends indicate the migration distance of cells into the scraped areas. Dashed lines show the initial leading edges. Scale bar, 100 µm.
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fig09: Inhibitory effect of CD133 antibody on cell migration. A migration assay was performed using the scratch wound healing assay and cells were viewed under an inverted optical microscope. a: Control. b: CD133 antibody treatment (10 μg/mL). Black lines with arrows at both ends indicate the migration distance of cells into the scraped areas. Dashed lines show the initial leading edges. Scale bar, 100 µm.

Mentions: As shown in Figure 9, cell migration decreased after treatment with a CD133 antibody (10 μg/mL) for 24 h. The distance of cell migration from the leading edge was significantly shortened by the CD133 antibody treatment (control group, 187.6 ± 16.0 μm; CD133 treatment group, 96.9 ± 8.2 μm, P < 0.01). However, the cell migration distance was not significantly affected at the lower antibody concentration (1 μg/mL).


Detection of CD133 (prominin-1) in a human hepatoblastoma cell line (HuH-6 clone 5).

Akita M, Tanaka K, Murai N, Matsumoto S, Fujita K, Takaki T, Nishiyama H - Microsc. Res. Tech. (2013)

Inhibitory effect of CD133 antibody on cell migration. A migration assay was performed using the scratch wound healing assay and cells were viewed under an inverted optical microscope. a: Control. b: CD133 antibody treatment (10 μg/mL). Black lines with arrows at both ends indicate the migration distance of cells into the scraped areas. Dashed lines show the initial leading edges. Scale bar, 100 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3842112&req=5

fig09: Inhibitory effect of CD133 antibody on cell migration. A migration assay was performed using the scratch wound healing assay and cells were viewed under an inverted optical microscope. a: Control. b: CD133 antibody treatment (10 μg/mL). Black lines with arrows at both ends indicate the migration distance of cells into the scraped areas. Dashed lines show the initial leading edges. Scale bar, 100 µm.
Mentions: As shown in Figure 9, cell migration decreased after treatment with a CD133 antibody (10 μg/mL) for 24 h. The distance of cell migration from the leading edge was significantly shortened by the CD133 antibody treatment (control group, 187.6 ± 16.0 μm; CD133 treatment group, 96.9 ± 8.2 μm, P < 0.01). However, the cell migration distance was not significantly affected at the lower antibody concentration (1 μg/mL).

Bottom Line: An antibody against CD133 decreased cell migration.Methyl-β-cyclodextrin treatment decreased cell adhesion as well as lamellipodium and filopodium formation.The results suggest that CD133 membrane localization plays a role in tumor cell adhesion and migration.

View Article: PubMed Central - PubMed

Affiliation: Division of Morphological Science, Biomedical Research Center, Saitama Medical University, Iruma-gun, Saitama, Japan. makita@saitama-med.ac.jp

Show MeSH
Related in: MedlinePlus