Detection of CD133 (prominin-1) in a human hepatoblastoma cell line (HuH-6 clone 5).
Bottom Line: An antibody against CD133 decreased cell migration.Methyl-β-cyclodextrin treatment decreased cell adhesion as well as lamellipodium and filopodium formation.The results suggest that CD133 membrane localization plays a role in tumor cell adhesion and migration.
Affiliation: Division of Morphological Science, Biomedical Research Center, Saitama Medical University, Iruma-gun, Saitama, Japan. email@example.comShow MeSH
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Mentions: MβCD treatment induced morphological changes in the cells. Cell–cell contact and cell spreading reduced, and many of the cells exhibited an oval shape (Figs. a–d). At the ultrastructural level, MβCD treatment decreased cell–cell contact (Fig. 11), and the CD133-positive cells acquired a round shape. Although lamellipodia and filopodia formation reduced on MβCD treatment, CD133 immunoreactivity was preserved (Figs.12 and 13).
Affiliation: Division of Morphological Science, Biomedical Research Center, Saitama Medical University, Iruma-gun, Saitama, Japan. firstname.lastname@example.org