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Detection of CD133 (prominin-1) in a human hepatoblastoma cell line (HuH-6 clone 5).

Akita M, Tanaka K, Murai N, Matsumoto S, Fujita K, Takaki T, Nishiyama H - Microsc. Res. Tech. (2013)

Bottom Line: An antibody against CD133 decreased cell migration.Methyl-β-cyclodextrin treatment decreased cell adhesion as well as lamellipodium and filopodium formation.The results suggest that CD133 membrane localization plays a role in tumor cell adhesion and migration.

View Article: PubMed Central - PubMed

Affiliation: Division of Morphological Science, Biomedical Research Center, Saitama Medical University, Iruma-gun, Saitama, Japan. makita@saitama-med.ac.jp

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Related in: MedlinePlus

Effect of methyl-β-cyclodextrin (MβCD) on cells. a, b: Control. Cells are well spread and cell–cell adhesion is well developed. a: Phase contrast microscopy; b: thin section stained with toluidine blue. c, d: MβCD treatment induces morphological changes in cells. Cell–cell adhesion is diminished and cells acquire a round shape. Scale bar, 40 µm (for b and d). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
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fig10: Effect of methyl-β-cyclodextrin (MβCD) on cells. a, b: Control. Cells are well spread and cell–cell adhesion is well developed. a: Phase contrast microscopy; b: thin section stained with toluidine blue. c, d: MβCD treatment induces morphological changes in cells. Cell–cell adhesion is diminished and cells acquire a round shape. Scale bar, 40 µm (for b and d). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Mentions: MβCD treatment induced morphological changes in the cells. Cell–cell contact and cell spreading reduced, and many of the cells exhibited an oval shape (Figs. a–d). At the ultrastructural level, MβCD treatment decreased cell–cell contact (Fig. 11), and the CD133-positive cells acquired a round shape. Although lamellipodia and filopodia formation reduced on MβCD treatment, CD133 immunoreactivity was preserved (Figs.12 and 13).


Detection of CD133 (prominin-1) in a human hepatoblastoma cell line (HuH-6 clone 5).

Akita M, Tanaka K, Murai N, Matsumoto S, Fujita K, Takaki T, Nishiyama H - Microsc. Res. Tech. (2013)

Effect of methyl-β-cyclodextrin (MβCD) on cells. a, b: Control. Cells are well spread and cell–cell adhesion is well developed. a: Phase contrast microscopy; b: thin section stained with toluidine blue. c, d: MβCD treatment induces morphological changes in cells. Cell–cell adhesion is diminished and cells acquire a round shape. Scale bar, 40 µm (for b and d). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3842112&req=5

fig10: Effect of methyl-β-cyclodextrin (MβCD) on cells. a, b: Control. Cells are well spread and cell–cell adhesion is well developed. a: Phase contrast microscopy; b: thin section stained with toluidine blue. c, d: MβCD treatment induces morphological changes in cells. Cell–cell adhesion is diminished and cells acquire a round shape. Scale bar, 40 µm (for b and d). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
Mentions: MβCD treatment induced morphological changes in the cells. Cell–cell contact and cell spreading reduced, and many of the cells exhibited an oval shape (Figs. a–d). At the ultrastructural level, MβCD treatment decreased cell–cell contact (Fig. 11), and the CD133-positive cells acquired a round shape. Although lamellipodia and filopodia formation reduced on MβCD treatment, CD133 immunoreactivity was preserved (Figs.12 and 13).

Bottom Line: An antibody against CD133 decreased cell migration.Methyl-β-cyclodextrin treatment decreased cell adhesion as well as lamellipodium and filopodium formation.The results suggest that CD133 membrane localization plays a role in tumor cell adhesion and migration.

View Article: PubMed Central - PubMed

Affiliation: Division of Morphological Science, Biomedical Research Center, Saitama Medical University, Iruma-gun, Saitama, Japan. makita@saitama-med.ac.jp

Show MeSH
Related in: MedlinePlus