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C. elegans sensing of and entrainment along obstacles require different neurons at different body locations.

Nam SW, Qian C, Kim SH, van Noort D, Chiam KH, Park S - Sci Rep (2013)

Bottom Line: We probe C. elegans mechanosensation using a microfabricated platform where worms encounter a linear array of asymmetric funnel-like barriers.We found that sensing of and moving along barriers require different sets of neurons located at different parts of the animal.Wild-type worms sense and move along the barrier walls, leading to their accumulation in one side of the barriers due to the barriers' asymmetric shape.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry and Nano Science, Ewha Global Top5 Research Program, Ewha Womans University, Seoul, Korea.

ABSTRACT
We probe C. elegans mechanosensation using a microfabricated platform where worms encounter a linear array of asymmetric funnel-like barriers. We found that sensing of and moving along barriers require different sets of neurons located at different parts of the animal. Wild-type worms sense and move along the barrier walls, leading to their accumulation in one side of the barriers due to the barriers' asymmetric shape. However, mec-4 and mec-10 mutants deficient in touch sensory neurons in the body exhibited reversal movements at the walls, leading to no accumulation in either side of the barriers. In contrast, osm-9 mutants deficient in touch sensory neurons in the nose, moved along the barrier walls. Thus, touch sensory neurons ALM and AVM in the body are required for C. elegans to sense and move along obstacles, whereas the ASH and FLP neurons in the nose are required only for sensing of but not moving along obstacles.

Show MeSH
The rectification of wild-type and mec-4 worms obtained from computational modeling.(A) Ratio of the density of worms in the right side of the funnels to all the worms in the chamber as a function of time. The ratios are averaged from 10 simulations, each of 50 worms and for 210 min. (B) Representative trajectory of a N2 worm. Color codes for time (blue: early; red: late). (C) Representative trajectory of a mec-4 mutant.
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f6: The rectification of wild-type and mec-4 worms obtained from computational modeling.(A) Ratio of the density of worms in the right side of the funnels to all the worms in the chamber as a function of time. The ratios are averaged from 10 simulations, each of 50 worms and for 210 min. (B) Representative trajectory of a N2 worm. Color codes for time (blue: early; red: late). (C) Representative trajectory of a mec-4 mutant.

Mentions: From the simulations, representative trajectories of wild-type and mec-4 worms are shown in Fig. 6B and 6C, respectively. The rectification for wild-type increased with time while mec-4 did not present such an effect (Fig. 6A), consistent with experimental results (Fig. 2B). The simulated trajectories are not deterministic, because they incorporate randomness in the form of reorientations at the wall, and thus, they also reflect the randomness observed in the experiments.


C. elegans sensing of and entrainment along obstacles require different neurons at different body locations.

Nam SW, Qian C, Kim SH, van Noort D, Chiam KH, Park S - Sci Rep (2013)

The rectification of wild-type and mec-4 worms obtained from computational modeling.(A) Ratio of the density of worms in the right side of the funnels to all the worms in the chamber as a function of time. The ratios are averaged from 10 simulations, each of 50 worms and for 210 min. (B) Representative trajectory of a N2 worm. Color codes for time (blue: early; red: late). (C) Representative trajectory of a mec-4 mutant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3842086&req=5

f6: The rectification of wild-type and mec-4 worms obtained from computational modeling.(A) Ratio of the density of worms in the right side of the funnels to all the worms in the chamber as a function of time. The ratios are averaged from 10 simulations, each of 50 worms and for 210 min. (B) Representative trajectory of a N2 worm. Color codes for time (blue: early; red: late). (C) Representative trajectory of a mec-4 mutant.
Mentions: From the simulations, representative trajectories of wild-type and mec-4 worms are shown in Fig. 6B and 6C, respectively. The rectification for wild-type increased with time while mec-4 did not present such an effect (Fig. 6A), consistent with experimental results (Fig. 2B). The simulated trajectories are not deterministic, because they incorporate randomness in the form of reorientations at the wall, and thus, they also reflect the randomness observed in the experiments.

Bottom Line: We probe C. elegans mechanosensation using a microfabricated platform where worms encounter a linear array of asymmetric funnel-like barriers.We found that sensing of and moving along barriers require different sets of neurons located at different parts of the animal.Wild-type worms sense and move along the barrier walls, leading to their accumulation in one side of the barriers due to the barriers' asymmetric shape.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry and Nano Science, Ewha Global Top5 Research Program, Ewha Womans University, Seoul, Korea.

ABSTRACT
We probe C. elegans mechanosensation using a microfabricated platform where worms encounter a linear array of asymmetric funnel-like barriers. We found that sensing of and moving along barriers require different sets of neurons located at different parts of the animal. Wild-type worms sense and move along the barrier walls, leading to their accumulation in one side of the barriers due to the barriers' asymmetric shape. However, mec-4 and mec-10 mutants deficient in touch sensory neurons in the body exhibited reversal movements at the walls, leading to no accumulation in either side of the barriers. In contrast, osm-9 mutants deficient in touch sensory neurons in the nose, moved along the barrier walls. Thus, touch sensory neurons ALM and AVM in the body are required for C. elegans to sense and move along obstacles, whereas the ASH and FLP neurons in the nose are required only for sensing of but not moving along obstacles.

Show MeSH