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C. elegans sensing of and entrainment along obstacles require different neurons at different body locations.

Nam SW, Qian C, Kim SH, van Noort D, Chiam KH, Park S - Sci Rep (2013)

Bottom Line: We probe C. elegans mechanosensation using a microfabricated platform where worms encounter a linear array of asymmetric funnel-like barriers.We found that sensing of and moving along barriers require different sets of neurons located at different parts of the animal.Wild-type worms sense and move along the barrier walls, leading to their accumulation in one side of the barriers due to the barriers' asymmetric shape.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry and Nano Science, Ewha Global Top5 Research Program, Ewha Womans University, Seoul, Korea.

ABSTRACT
We probe C. elegans mechanosensation using a microfabricated platform where worms encounter a linear array of asymmetric funnel-like barriers. We found that sensing of and moving along barriers require different sets of neurons located at different parts of the animal. Wild-type worms sense and move along the barrier walls, leading to their accumulation in one side of the barriers due to the barriers' asymmetric shape. However, mec-4 and mec-10 mutants deficient in touch sensory neurons in the body exhibited reversal movements at the walls, leading to no accumulation in either side of the barriers. In contrast, osm-9 mutants deficient in touch sensory neurons in the nose, moved along the barrier walls. Thus, touch sensory neurons ALM and AVM in the body are required for C. elegans to sense and move along obstacles, whereas the ASH and FLP neurons in the nose are required only for sensing of but not moving along obstacles.

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Relation between degenerin/Na+ channel and rectification behavior of C. elegans.(A) Effect of degenerin/Na+ channel blocker, amiloride, on the density ratio after 180 min. Asterisks denote values that are different from the value of mec-4 and mec-10 without treatment of amiloride at p < 0.0001 (One-way ANOVA with Bonferroni post-tests analysis). The ratio was given as the mean ± standard deviation (s.d.) of 4 independent experiments (each experiment with 50–60 young adult worms, each worm of length between 0.7 mm and 0.8 mm long). (B) Schematic drawing of the putative relationship between degenerin/Na+ channel and rectification behavior of C. elegans.
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f5: Relation between degenerin/Na+ channel and rectification behavior of C. elegans.(A) Effect of degenerin/Na+ channel blocker, amiloride, on the density ratio after 180 min. Asterisks denote values that are different from the value of mec-4 and mec-10 without treatment of amiloride at p < 0.0001 (One-way ANOVA with Bonferroni post-tests analysis). The ratio was given as the mean ± standard deviation (s.d.) of 4 independent experiments (each experiment with 50–60 young adult worms, each worm of length between 0.7 mm and 0.8 mm long). (B) Schematic drawing of the putative relationship between degenerin/Na+ channel and rectification behavior of C. elegans.

Mentions: The mec-4 and mec-10 genes encode their respective subunits of a mechanically-gated degenerin/Na+ channel in the touch sensory neurons in the body1215161718. In order to elucidate whether the mutated degenerin/Na+ channels are responsible for the lack of wall entrainment and reversal at the walls, the mec mutants were treated with amiloride, a degenerin/Na+ channel blocker16. After incubation with 0.3 mM amiloride for 14 hrs, mec-4 and mec-10 exhibited rectification, just like wild-type (Fig. 5A).


C. elegans sensing of and entrainment along obstacles require different neurons at different body locations.

Nam SW, Qian C, Kim SH, van Noort D, Chiam KH, Park S - Sci Rep (2013)

Relation between degenerin/Na+ channel and rectification behavior of C. elegans.(A) Effect of degenerin/Na+ channel blocker, amiloride, on the density ratio after 180 min. Asterisks denote values that are different from the value of mec-4 and mec-10 without treatment of amiloride at p < 0.0001 (One-way ANOVA with Bonferroni post-tests analysis). The ratio was given as the mean ± standard deviation (s.d.) of 4 independent experiments (each experiment with 50–60 young adult worms, each worm of length between 0.7 mm and 0.8 mm long). (B) Schematic drawing of the putative relationship between degenerin/Na+ channel and rectification behavior of C. elegans.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3842086&req=5

f5: Relation between degenerin/Na+ channel and rectification behavior of C. elegans.(A) Effect of degenerin/Na+ channel blocker, amiloride, on the density ratio after 180 min. Asterisks denote values that are different from the value of mec-4 and mec-10 without treatment of amiloride at p < 0.0001 (One-way ANOVA with Bonferroni post-tests analysis). The ratio was given as the mean ± standard deviation (s.d.) of 4 independent experiments (each experiment with 50–60 young adult worms, each worm of length between 0.7 mm and 0.8 mm long). (B) Schematic drawing of the putative relationship between degenerin/Na+ channel and rectification behavior of C. elegans.
Mentions: The mec-4 and mec-10 genes encode their respective subunits of a mechanically-gated degenerin/Na+ channel in the touch sensory neurons in the body1215161718. In order to elucidate whether the mutated degenerin/Na+ channels are responsible for the lack of wall entrainment and reversal at the walls, the mec mutants were treated with amiloride, a degenerin/Na+ channel blocker16. After incubation with 0.3 mM amiloride for 14 hrs, mec-4 and mec-10 exhibited rectification, just like wild-type (Fig. 5A).

Bottom Line: We probe C. elegans mechanosensation using a microfabricated platform where worms encounter a linear array of asymmetric funnel-like barriers.We found that sensing of and moving along barriers require different sets of neurons located at different parts of the animal.Wild-type worms sense and move along the barrier walls, leading to their accumulation in one side of the barriers due to the barriers' asymmetric shape.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry and Nano Science, Ewha Global Top5 Research Program, Ewha Womans University, Seoul, Korea.

ABSTRACT
We probe C. elegans mechanosensation using a microfabricated platform where worms encounter a linear array of asymmetric funnel-like barriers. We found that sensing of and moving along barriers require different sets of neurons located at different parts of the animal. Wild-type worms sense and move along the barrier walls, leading to their accumulation in one side of the barriers due to the barriers' asymmetric shape. However, mec-4 and mec-10 mutants deficient in touch sensory neurons in the body exhibited reversal movements at the walls, leading to no accumulation in either side of the barriers. In contrast, osm-9 mutants deficient in touch sensory neurons in the nose, moved along the barrier walls. Thus, touch sensory neurons ALM and AVM in the body are required for C. elegans to sense and move along obstacles, whereas the ASH and FLP neurons in the nose are required only for sensing of but not moving along obstacles.

Show MeSH