Limits...
Mesoporous silicon microparticles enhance MHC class I cross-antigen presentation by human dendritic cells.

Jiménez-Periáñez A, Abos Gracia B, López Relaño J, Diez-Rivero CM, Reche PA, Martínez-Naves E, Matveyeva E, Gómez del Moral M - Clin. Dev. Immunol. (2013)

Bottom Line: The mesoporous silicon microparticles (MSMPs) are excellent vehicles for releasing molecules inside the cell.The aim of this work was to use MSMPs to deliver viral specific MHC class I restricted epitopes into human antigen presenting cells (monocyte derived dendritic cells, MDDCs) to facilitate their capture, processing, and presentation to CD8+ (cytotoxic) T lymphocytes.We show for the first time that MSMPs vehiculation of antigenic peptides enhances their MHC class I presentation by human MDDCs to CD8 T lymphocytes.

View Article: PubMed Central - PubMed

Affiliation: EM-Silicon Nano-Technologies SL, Nat. R. Cisternas 8, 46010 Valencia, Spain.

ABSTRACT
The mesoporous silicon microparticles (MSMPs) are excellent vehicles for releasing molecules inside the cell. The aim of this work was to use MSMPs to deliver viral specific MHC class I restricted epitopes into human antigen presenting cells (monocyte derived dendritic cells, MDDCs) to facilitate their capture, processing, and presentation to CD8+ (cytotoxic) T lymphocytes. We show for the first time that MSMPs vehiculation of antigenic peptides enhances their MHC class I presentation by human MDDCs to CD8 T lymphocytes.

Show MeSH
The microparticles are internalized into dendritic cells (DC). (a) Photographs show control cells (MDDCs) or with internalized unloaded (MDDCs-MSMPs) or peptide loaded (MDDCs-MSMPs-CEFpp) microparticles. After 24 h of incubation the cells are aggregated, and the particles are inside vacuoles ((b)-(c) and insert). Trypan blue dye was used to determine the percentage of live MDDCs relative to a nontreatment control following 24 h incubation with MSMPs (d). *P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3842076&req=5

fig3: The microparticles are internalized into dendritic cells (DC). (a) Photographs show control cells (MDDCs) or with internalized unloaded (MDDCs-MSMPs) or peptide loaded (MDDCs-MSMPs-CEFpp) microparticles. After 24 h of incubation the cells are aggregated, and the particles are inside vacuoles ((b)-(c) and insert). Trypan blue dye was used to determine the percentage of live MDDCs relative to a nontreatment control following 24 h incubation with MSMPs (d). *P < 0.05.

Mentions: After ensuring that MSMPs were not contaminated with endotoxin (see Figure S1 in Supplementary Material), initial experiments were performed to determine the best microparticle-MDDC incubation conditions to accurately measure and visualize intracellular microparticles. The best microparticle-MDDC ratio was 10 : 1. After 2 h approximately 30% of MDDCs contained embedded microparticles in membrane veils and endosomes. At 24 h large MDDC clusters with multiple engulfed microparticles in endosomes could be observed under light microscope. At this time 100% of human MDDCs had microparticles engulfed (Figures 3(a)–3(c)). Flow cytometry detected great differences in the complexity (SSC) of MDDCs exposed to microparticles compared to unexposed MDDCs, no differences were observed in size (FSC) between exposed or not MDDCs (see Figure S2 in Supplementary Material).


Mesoporous silicon microparticles enhance MHC class I cross-antigen presentation by human dendritic cells.

Jiménez-Periáñez A, Abos Gracia B, López Relaño J, Diez-Rivero CM, Reche PA, Martínez-Naves E, Matveyeva E, Gómez del Moral M - Clin. Dev. Immunol. (2013)

The microparticles are internalized into dendritic cells (DC). (a) Photographs show control cells (MDDCs) or with internalized unloaded (MDDCs-MSMPs) or peptide loaded (MDDCs-MSMPs-CEFpp) microparticles. After 24 h of incubation the cells are aggregated, and the particles are inside vacuoles ((b)-(c) and insert). Trypan blue dye was used to determine the percentage of live MDDCs relative to a nontreatment control following 24 h incubation with MSMPs (d). *P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3842076&req=5

fig3: The microparticles are internalized into dendritic cells (DC). (a) Photographs show control cells (MDDCs) or with internalized unloaded (MDDCs-MSMPs) or peptide loaded (MDDCs-MSMPs-CEFpp) microparticles. After 24 h of incubation the cells are aggregated, and the particles are inside vacuoles ((b)-(c) and insert). Trypan blue dye was used to determine the percentage of live MDDCs relative to a nontreatment control following 24 h incubation with MSMPs (d). *P < 0.05.
Mentions: After ensuring that MSMPs were not contaminated with endotoxin (see Figure S1 in Supplementary Material), initial experiments were performed to determine the best microparticle-MDDC incubation conditions to accurately measure and visualize intracellular microparticles. The best microparticle-MDDC ratio was 10 : 1. After 2 h approximately 30% of MDDCs contained embedded microparticles in membrane veils and endosomes. At 24 h large MDDC clusters with multiple engulfed microparticles in endosomes could be observed under light microscope. At this time 100% of human MDDCs had microparticles engulfed (Figures 3(a)–3(c)). Flow cytometry detected great differences in the complexity (SSC) of MDDCs exposed to microparticles compared to unexposed MDDCs, no differences were observed in size (FSC) between exposed or not MDDCs (see Figure S2 in Supplementary Material).

Bottom Line: The mesoporous silicon microparticles (MSMPs) are excellent vehicles for releasing molecules inside the cell.The aim of this work was to use MSMPs to deliver viral specific MHC class I restricted epitopes into human antigen presenting cells (monocyte derived dendritic cells, MDDCs) to facilitate their capture, processing, and presentation to CD8+ (cytotoxic) T lymphocytes.We show for the first time that MSMPs vehiculation of antigenic peptides enhances their MHC class I presentation by human MDDCs to CD8 T lymphocytes.

View Article: PubMed Central - PubMed

Affiliation: EM-Silicon Nano-Technologies SL, Nat. R. Cisternas 8, 46010 Valencia, Spain.

ABSTRACT
The mesoporous silicon microparticles (MSMPs) are excellent vehicles for releasing molecules inside the cell. The aim of this work was to use MSMPs to deliver viral specific MHC class I restricted epitopes into human antigen presenting cells (monocyte derived dendritic cells, MDDCs) to facilitate their capture, processing, and presentation to CD8+ (cytotoxic) T lymphocytes. We show for the first time that MSMPs vehiculation of antigenic peptides enhances their MHC class I presentation by human MDDCs to CD8 T lymphocytes.

Show MeSH