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Mesoporous silicon microparticles enhance MHC class I cross-antigen presentation by human dendritic cells.

Jiménez-Periáñez A, Abos Gracia B, López Relaño J, Diez-Rivero CM, Reche PA, Martínez-Naves E, Matveyeva E, Gómez del Moral M - Clin. Dev. Immunol. (2013)

Bottom Line: The mesoporous silicon microparticles (MSMPs) are excellent vehicles for releasing molecules inside the cell.The aim of this work was to use MSMPs to deliver viral specific MHC class I restricted epitopes into human antigen presenting cells (monocyte derived dendritic cells, MDDCs) to facilitate their capture, processing, and presentation to CD8+ (cytotoxic) T lymphocytes.We show for the first time that MSMPs vehiculation of antigenic peptides enhances their MHC class I presentation by human MDDCs to CD8 T lymphocytes.

View Article: PubMed Central - PubMed

Affiliation: EM-Silicon Nano-Technologies SL, Nat. R. Cisternas 8, 46010 Valencia, Spain.

ABSTRACT
The mesoporous silicon microparticles (MSMPs) are excellent vehicles for releasing molecules inside the cell. The aim of this work was to use MSMPs to deliver viral specific MHC class I restricted epitopes into human antigen presenting cells (monocyte derived dendritic cells, MDDCs) to facilitate their capture, processing, and presentation to CD8+ (cytotoxic) T lymphocytes. We show for the first time that MSMPs vehiculation of antigenic peptides enhances their MHC class I presentation by human MDDCs to CD8 T lymphocytes.

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The scanning electron microscopy photos of the as-prepared porous silicon material: (a) in-plan view of the treated silicon wafer (×50.000), (b) transversal view of the porous material (×5.000), (c) particle surface after grinding (×50.000).
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fig2: The scanning electron microscopy photos of the as-prepared porous silicon material: (a) in-plan view of the treated silicon wafer (×50.000), (b) transversal view of the porous material (×5.000), (c) particle surface after grinding (×50.000).

Mentions: The surface area and pore size distribution were analyzed by nitrogen adsorption/desorption method (77.1 K, Micrometrics ASAP2000 volumetric analyser). Measurements were performed prior to the milling and after the milling and cells assays; samples were out-gassed under dynamic vacuum overnight at 130°C (for initial not milled material) or at only 37°C (after bioassays). Both the BET (Brunauer-Emmett-Teller) and the BJH (Barrett-Joyner-Halenda) approximations were used to calculate surface areas. The pore size distribution curves were calculated using the BJH method. Using the total volumes of adsorbed nitrogen and the weight of the porous sample used in each analysis, we were able to calculate the porosity as well. This method yielded values of 65–67%, quite similar to that obtained by gravimetric method. All the calculated data are presented in Table 1. The morphology of the initial material was visualized with the high resolution Scanning Electron Microscopy (SEM, Hitachi S4500). In Figure 2, the SEM images of the fabricated material are shown: (a) before scratching of the porous layer out from the Si wafer, (b) the transversal view of a big fragment of porous material and (c) the individual particle after milling. The pores of less than 20 nm penetrated throughout the material are clearly seen on these photos.


Mesoporous silicon microparticles enhance MHC class I cross-antigen presentation by human dendritic cells.

Jiménez-Periáñez A, Abos Gracia B, López Relaño J, Diez-Rivero CM, Reche PA, Martínez-Naves E, Matveyeva E, Gómez del Moral M - Clin. Dev. Immunol. (2013)

The scanning electron microscopy photos of the as-prepared porous silicon material: (a) in-plan view of the treated silicon wafer (×50.000), (b) transversal view of the porous material (×5.000), (c) particle surface after grinding (×50.000).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3842076&req=5

fig2: The scanning electron microscopy photos of the as-prepared porous silicon material: (a) in-plan view of the treated silicon wafer (×50.000), (b) transversal view of the porous material (×5.000), (c) particle surface after grinding (×50.000).
Mentions: The surface area and pore size distribution were analyzed by nitrogen adsorption/desorption method (77.1 K, Micrometrics ASAP2000 volumetric analyser). Measurements were performed prior to the milling and after the milling and cells assays; samples were out-gassed under dynamic vacuum overnight at 130°C (for initial not milled material) or at only 37°C (after bioassays). Both the BET (Brunauer-Emmett-Teller) and the BJH (Barrett-Joyner-Halenda) approximations were used to calculate surface areas. The pore size distribution curves were calculated using the BJH method. Using the total volumes of adsorbed nitrogen and the weight of the porous sample used in each analysis, we were able to calculate the porosity as well. This method yielded values of 65–67%, quite similar to that obtained by gravimetric method. All the calculated data are presented in Table 1. The morphology of the initial material was visualized with the high resolution Scanning Electron Microscopy (SEM, Hitachi S4500). In Figure 2, the SEM images of the fabricated material are shown: (a) before scratching of the porous layer out from the Si wafer, (b) the transversal view of a big fragment of porous material and (c) the individual particle after milling. The pores of less than 20 nm penetrated throughout the material are clearly seen on these photos.

Bottom Line: The mesoporous silicon microparticles (MSMPs) are excellent vehicles for releasing molecules inside the cell.The aim of this work was to use MSMPs to deliver viral specific MHC class I restricted epitopes into human antigen presenting cells (monocyte derived dendritic cells, MDDCs) to facilitate their capture, processing, and presentation to CD8+ (cytotoxic) T lymphocytes.We show for the first time that MSMPs vehiculation of antigenic peptides enhances their MHC class I presentation by human MDDCs to CD8 T lymphocytes.

View Article: PubMed Central - PubMed

Affiliation: EM-Silicon Nano-Technologies SL, Nat. R. Cisternas 8, 46010 Valencia, Spain.

ABSTRACT
The mesoporous silicon microparticles (MSMPs) are excellent vehicles for releasing molecules inside the cell. The aim of this work was to use MSMPs to deliver viral specific MHC class I restricted epitopes into human antigen presenting cells (monocyte derived dendritic cells, MDDCs) to facilitate their capture, processing, and presentation to CD8+ (cytotoxic) T lymphocytes. We show for the first time that MSMPs vehiculation of antigenic peptides enhances their MHC class I presentation by human MDDCs to CD8 T lymphocytes.

Show MeSH