Limits...
Tyrosine phosphorylation modulates the vascular responses of mesenteric arteries from human colorectal tumors.

Ferrero E, Mauricio MD, Granado M, García-Villar O, Aldasoro M, Vila JM, Hidalgo M, Ferrero JL, Fernández N, Monge L, García-Villalón AL - Biomed Res Int (2013)

Bottom Line: Increasing tyrosine phosphorylation with the phosphatase inhibitor, sodium orthovanadate produced arterial contraction which was lower in tumoral than in control arteries, whereas it reduced the contraction to noradrenaline in tumoral but not in control arteries and reduced the relaxation to bradykinin in control but not in tumoral arteries.Protein expression of VEGF-A and of the VEGF receptor FLT1 was similar in control and tumoral arteries, but expression of the VEGF receptor KDR was increased in tumoral compared with control arteries.This suggests that tyrosine phosphorylation may produce inhibition of the contraction in tumoral mesenteric arteries, which may increase blood flow to the tumor when tyrosine phosphorylation is increased by stimulation of VEGF receptors.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Cirugía General y Digestiva (Seccion B), Hospital Universitario "12 de Octubre", Universidad Complutense, Avenida de Córdoba, s/n, 28041 Madrid, Spain.

ABSTRACT
The aim of this study was to analyze whether tyrosine phosphorylation in tumoral arteries may modulate their vascular response. To do this, mesenteric arteries supplying blood flow to colorectal tumors or to normal intestine were obtained during surgery and prepared for isometric tension recording in an organ bath. Increasing tyrosine phosphorylation with the phosphatase inhibitor, sodium orthovanadate produced arterial contraction which was lower in tumoral than in control arteries, whereas it reduced the contraction to noradrenaline in tumoral but not in control arteries and reduced the relaxation to bradykinin in control but not in tumoral arteries. Protein expression of VEGF-A and of the VEGF receptor FLT1 was similar in control and tumoral arteries, but expression of the VEGF receptor KDR was increased in tumoral compared with control arteries. This suggests that tyrosine phosphorylation may produce inhibition of the contraction in tumoral mesenteric arteries, which may increase blood flow to the tumor when tyrosine phosphorylation is increased by stimulation of VEGF receptors.

Show MeSH

Related in: MedlinePlus

RT-PCR analysis of mRNA expression for VEGF-A, FLT1, and KDR in mesenteric arteries supplying colorectal tumours (tumour, n = 6) and those supplying normal colon (control, n = 6). Values were normalized to GAPDH mRNA expression. Data are presented as mean ± SEM. *P < 0.05 statistically significant versus control samples.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3842070&req=5

fig4: RT-PCR analysis of mRNA expression for VEGF-A, FLT1, and KDR in mesenteric arteries supplying colorectal tumours (tumour, n = 6) and those supplying normal colon (control, n = 6). Values were normalized to GAPDH mRNA expression. Data are presented as mean ± SEM. *P < 0.05 statistically significant versus control samples.

Mentions: Quantitative PCR-RT analysis revealed a significant decrease in VEGF-A expression in tumor samples compared to the control (1.18 ± 0.20 versus 0.36 ± 0.06, P < 0.05). We have also determined the expression of KDR and FLT1, the main receptors of VEGF-A in the arteries [20]. Compared with the control, the vascular mRNA gene expression of KDR and FLT1 from tumor samples was significantly downregulated (60% and 64%, resp., P < 0.05). As a control, the expression of GAPDH was measured in parallel using the same mRNA samples (Figure 4).


Tyrosine phosphorylation modulates the vascular responses of mesenteric arteries from human colorectal tumors.

Ferrero E, Mauricio MD, Granado M, García-Villar O, Aldasoro M, Vila JM, Hidalgo M, Ferrero JL, Fernández N, Monge L, García-Villalón AL - Biomed Res Int (2013)

RT-PCR analysis of mRNA expression for VEGF-A, FLT1, and KDR in mesenteric arteries supplying colorectal tumours (tumour, n = 6) and those supplying normal colon (control, n = 6). Values were normalized to GAPDH mRNA expression. Data are presented as mean ± SEM. *P < 0.05 statistically significant versus control samples.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3842070&req=5

fig4: RT-PCR analysis of mRNA expression for VEGF-A, FLT1, and KDR in mesenteric arteries supplying colorectal tumours (tumour, n = 6) and those supplying normal colon (control, n = 6). Values were normalized to GAPDH mRNA expression. Data are presented as mean ± SEM. *P < 0.05 statistically significant versus control samples.
Mentions: Quantitative PCR-RT analysis revealed a significant decrease in VEGF-A expression in tumor samples compared to the control (1.18 ± 0.20 versus 0.36 ± 0.06, P < 0.05). We have also determined the expression of KDR and FLT1, the main receptors of VEGF-A in the arteries [20]. Compared with the control, the vascular mRNA gene expression of KDR and FLT1 from tumor samples was significantly downregulated (60% and 64%, resp., P < 0.05). As a control, the expression of GAPDH was measured in parallel using the same mRNA samples (Figure 4).

Bottom Line: Increasing tyrosine phosphorylation with the phosphatase inhibitor, sodium orthovanadate produced arterial contraction which was lower in tumoral than in control arteries, whereas it reduced the contraction to noradrenaline in tumoral but not in control arteries and reduced the relaxation to bradykinin in control but not in tumoral arteries.Protein expression of VEGF-A and of the VEGF receptor FLT1 was similar in control and tumoral arteries, but expression of the VEGF receptor KDR was increased in tumoral compared with control arteries.This suggests that tyrosine phosphorylation may produce inhibition of the contraction in tumoral mesenteric arteries, which may increase blood flow to the tumor when tyrosine phosphorylation is increased by stimulation of VEGF receptors.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Cirugía General y Digestiva (Seccion B), Hospital Universitario "12 de Octubre", Universidad Complutense, Avenida de Córdoba, s/n, 28041 Madrid, Spain.

ABSTRACT
The aim of this study was to analyze whether tyrosine phosphorylation in tumoral arteries may modulate their vascular response. To do this, mesenteric arteries supplying blood flow to colorectal tumors or to normal intestine were obtained during surgery and prepared for isometric tension recording in an organ bath. Increasing tyrosine phosphorylation with the phosphatase inhibitor, sodium orthovanadate produced arterial contraction which was lower in tumoral than in control arteries, whereas it reduced the contraction to noradrenaline in tumoral but not in control arteries and reduced the relaxation to bradykinin in control but not in tumoral arteries. Protein expression of VEGF-A and of the VEGF receptor FLT1 was similar in control and tumoral arteries, but expression of the VEGF receptor KDR was increased in tumoral compared with control arteries. This suggests that tyrosine phosphorylation may produce inhibition of the contraction in tumoral mesenteric arteries, which may increase blood flow to the tumor when tyrosine phosphorylation is increased by stimulation of VEGF receptors.

Show MeSH
Related in: MedlinePlus