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Killer cell immunoglobulin-like receptor (KIR) genes and their HLA-C ligands in a Ugandan population.

Nakimuli A, Chazara O, Farrell L, Hiby SE, Tukwasibwe S, Knee O, Jayaraman J, Traherne JA, Elliott AM, Kaleebu P, Mirembe F, Moffett A - Immunogenetics (2013)

Bottom Line: We studied the frequencies of KIR genes and HLA-C1 and C2 groups in a large cohort (n = 492) from Kampala, Uganda, East Africa and compared our findings with published data from other populations in sub-Saharan Africa (SSA) and several European populations.C1 and C2 frequencies were similar to other SSA populations with a higher frequency of the C2 epitope (54.9 %) compared to Europe (average 39.7 %).Our results will help understand how KIR/HLA-C interactions contribute to resistance to pathogens and reproductive success.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Makerere University, Kampala, Uganda.

ABSTRACT
Killer cell immunoglobulin-like receptor (KIR) genes are expressed by natural killer cells and encoded by a family of genes exhibiting considerable haplotypic and allelic variation. HLA-C molecules, the dominant ligands for KIR, are present in all individuals and are discriminated by two KIR epitopes, C1 and C2. We studied the frequencies of KIR genes and HLA-C1 and C2 groups in a large cohort (n = 492) from Kampala, Uganda, East Africa and compared our findings with published data from other populations in sub-Saharan Africa (SSA) and several European populations. We find considerably more KIR diversity and weaker linkage disequilibrium in SSA compared to the European populations and describe several novel KIR genotypes. C1 and C2 frequencies were similar to other SSA populations with a higher frequency of the C2 epitope (54.9 %) compared to Europe (average 39.7 %). Analysis of this large cohort from Uganda in the context of other African populations reveals variations in KIR and HLA-C1 and C2 that are consistent with migrations within Africa and potential selection pressures on these genes. Our results will help understand how KIR/HLA-C interactions contribute to resistance to pathogens and reproductive success.

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Related in: MedlinePlus

KIR genotype profiles and frequencies observed in the Ugandan population. Reference numbers are according to the Allele Frequency Net database. New genotypes identified in this study are described as “novel.” Genes are presented in the order observed on sequenced KIR haplotypes except for KIR2DS3S5 and KIR2DL5. Inhibitory genes are in red, activating genes are in blue, and the pseudo gene KIR2DP1 is in gray
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Fig3: KIR genotype profiles and frequencies observed in the Ugandan population. Reference numbers are according to the Allele Frequency Net database. New genotypes identified in this study are described as “novel.” Genes are presented in the order observed on sequenced KIR haplotypes except for KIR2DS3S5 and KIR2DL5. Inhibitory genes are in red, activating genes are in blue, and the pseudo gene KIR2DP1 is in gray

Mentions: Genotyping of the entire cohort for the presence/absence of the 12 variable KIR genes allowed the definition of individual KIR genotypes. Fifty-two KIR gene content profiles were observed in the Ugandan population (Fig. 3). Nineteen genotypes had a frequency above 1 %, accounting for 90.0 % of individuals. Half of the individuals were classified as Ganda (both parents speaking the Ganda language, 50.9 %), but the other groups were broadly similar in KIR genotype frequencies (Table S 1). As in other African populations, the most frequent KIR genotype in the Ugandan population is homozygosity for the KIR A haplotype (28.1 %). The reported frequencies for this KIR AA genotype in Africa are variable, from 12 % in a South Africa Xhosa population to 42 % in Senegal (Williams et al. 2004; Yindom et al. 2010).Fig. 3


Killer cell immunoglobulin-like receptor (KIR) genes and their HLA-C ligands in a Ugandan population.

Nakimuli A, Chazara O, Farrell L, Hiby SE, Tukwasibwe S, Knee O, Jayaraman J, Traherne JA, Elliott AM, Kaleebu P, Mirembe F, Moffett A - Immunogenetics (2013)

KIR genotype profiles and frequencies observed in the Ugandan population. Reference numbers are according to the Allele Frequency Net database. New genotypes identified in this study are described as “novel.” Genes are presented in the order observed on sequenced KIR haplotypes except for KIR2DS3S5 and KIR2DL5. Inhibitory genes are in red, activating genes are in blue, and the pseudo gene KIR2DP1 is in gray
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3824577&req=5

Fig3: KIR genotype profiles and frequencies observed in the Ugandan population. Reference numbers are according to the Allele Frequency Net database. New genotypes identified in this study are described as “novel.” Genes are presented in the order observed on sequenced KIR haplotypes except for KIR2DS3S5 and KIR2DL5. Inhibitory genes are in red, activating genes are in blue, and the pseudo gene KIR2DP1 is in gray
Mentions: Genotyping of the entire cohort for the presence/absence of the 12 variable KIR genes allowed the definition of individual KIR genotypes. Fifty-two KIR gene content profiles were observed in the Ugandan population (Fig. 3). Nineteen genotypes had a frequency above 1 %, accounting for 90.0 % of individuals. Half of the individuals were classified as Ganda (both parents speaking the Ganda language, 50.9 %), but the other groups were broadly similar in KIR genotype frequencies (Table S 1). As in other African populations, the most frequent KIR genotype in the Ugandan population is homozygosity for the KIR A haplotype (28.1 %). The reported frequencies for this KIR AA genotype in Africa are variable, from 12 % in a South Africa Xhosa population to 42 % in Senegal (Williams et al. 2004; Yindom et al. 2010).Fig. 3

Bottom Line: We studied the frequencies of KIR genes and HLA-C1 and C2 groups in a large cohort (n = 492) from Kampala, Uganda, East Africa and compared our findings with published data from other populations in sub-Saharan Africa (SSA) and several European populations.C1 and C2 frequencies were similar to other SSA populations with a higher frequency of the C2 epitope (54.9 %) compared to Europe (average 39.7 %).Our results will help understand how KIR/HLA-C interactions contribute to resistance to pathogens and reproductive success.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Makerere University, Kampala, Uganda.

ABSTRACT
Killer cell immunoglobulin-like receptor (KIR) genes are expressed by natural killer cells and encoded by a family of genes exhibiting considerable haplotypic and allelic variation. HLA-C molecules, the dominant ligands for KIR, are present in all individuals and are discriminated by two KIR epitopes, C1 and C2. We studied the frequencies of KIR genes and HLA-C1 and C2 groups in a large cohort (n = 492) from Kampala, Uganda, East Africa and compared our findings with published data from other populations in sub-Saharan Africa (SSA) and several European populations. We find considerably more KIR diversity and weaker linkage disequilibrium in SSA compared to the European populations and describe several novel KIR genotypes. C1 and C2 frequencies were similar to other SSA populations with a higher frequency of the C2 epitope (54.9 %) compared to Europe (average 39.7 %). Analysis of this large cohort from Uganda in the context of other African populations reveals variations in KIR and HLA-C1 and C2 that are consistent with migrations within Africa and potential selection pressures on these genes. Our results will help understand how KIR/HLA-C interactions contribute to resistance to pathogens and reproductive success.

Show MeSH
Related in: MedlinePlus