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Quantitative analysis of betaine in Lycii Fructus by HILIC-ELSD.

Zhao BT, Jeong SY, Hwangbo K, Moon DC, Seo EK, Lee D, Lee JH, Min BS, Ma ES, Son JK, Woo MH - Arch. Pharm. Res. (2013)

Bottom Line: Betaine was separated with an Atlantis hydrophilic interaction liquid chromatography silica column (4.6 × 150 mm, 5 μm, 100 Å) by isocratic elution using 30 mM ammonium acetate buffer and acetonitrile (20:80, v/v %) as the mobile phase.The method was fully validated with respect to linearity, precision, accuracy, stability and robustness.The HPLC/ELSD method was applied successfully to the quantification of betaine in the extract of Lycii Fructus.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, South Korea.

ABSTRACT
A rapid and simple high-performance liquid chromatography (HPLC) method with evaporative light scattering detection (ELSD) was developed for the determination of betaine from Lycii Fructus. Betaine was separated with an Atlantis hydrophilic interaction liquid chromatography silica column (4.6 × 150 mm, 5 μm, 100 Å) by isocratic elution using 30 mM ammonium acetate buffer and acetonitrile (20:80, v/v %) as the mobile phase. The flow rate was 1.0 mL/min, and the temperature for the spray chamber and drift tube was set at 30 and 50 °C, respectively. The method was fully validated with respect to linearity, precision, accuracy, stability and robustness. The HPLC/ELSD method was applied successfully to the quantification of betaine in the extract of Lycii Fructus. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of twenty-six L. barbarum L. from China (BC01-BC26), 3 L. barbarum L. (BJ27-BJ29) from Japan, 12 L. chinense Miller from China (CC30-CC41) and 51 L. chinense Miller samples (CK42-CK92) from Korea. The results indicate that the established HPLC/ELSD method is suitable for quality evaluation of Lycii Fructus.

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HClust of 92 authentic specimens of Lycii Fructus. Group A (empty circle): L. chinense Miller (CC30–CC41 and CK42–CK92), Group B (filled circle): L. barbarum L. (BC01–BC26 and BJ27–BJ29)
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Fig4: HClust of 92 authentic specimens of Lycii Fructus. Group A (empty circle): L. chinense Miller (CC30–CC41 and CK42–CK92), Group B (filled circle): L. barbarum L. (BC01–BC26 and BJ27–BJ29)

Mentions: To evaluate the phytochemical equivalency among the 26 L. barbarum L. from China (BC01–BC26), 3 L. barbarum L. (BJ27–BJ29) from Japan, 12 L. chinense Miller from China (CC30–CC41), and 51 L. chinense Miller samples (CK42–CK92) from Korea, pattern recognition analysis was conducted using the peak areas of betaine and one unknown peaks. In the pattern recognition analysis we indicated that all of the samples were largely clustered into two groups, A (L. chinense Miller) and B (L. barbarum L.). This result revealed that the pattern analysis of Lycii Fructus samples had a relationship with species (L. barbarum L. and L. chinense Miller) regardless of geographical origin (Fig. 4). Interestingly, although the contents of betaine were similar in the two species of L. barbarum L. and L. chinense Miller, Hclust could distinguish between L. barbarum L. and L. chinense Miller. Hence, this pattern analysis results could be used for the quality control of Lycii Fructus.Fig. 4


Quantitative analysis of betaine in Lycii Fructus by HILIC-ELSD.

Zhao BT, Jeong SY, Hwangbo K, Moon DC, Seo EK, Lee D, Lee JH, Min BS, Ma ES, Son JK, Woo MH - Arch. Pharm. Res. (2013)

HClust of 92 authentic specimens of Lycii Fructus. Group A (empty circle): L. chinense Miller (CC30–CC41 and CK42–CK92), Group B (filled circle): L. barbarum L. (BC01–BC26 and BJ27–BJ29)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3824576&req=5

Fig4: HClust of 92 authentic specimens of Lycii Fructus. Group A (empty circle): L. chinense Miller (CC30–CC41 and CK42–CK92), Group B (filled circle): L. barbarum L. (BC01–BC26 and BJ27–BJ29)
Mentions: To evaluate the phytochemical equivalency among the 26 L. barbarum L. from China (BC01–BC26), 3 L. barbarum L. (BJ27–BJ29) from Japan, 12 L. chinense Miller from China (CC30–CC41), and 51 L. chinense Miller samples (CK42–CK92) from Korea, pattern recognition analysis was conducted using the peak areas of betaine and one unknown peaks. In the pattern recognition analysis we indicated that all of the samples were largely clustered into two groups, A (L. chinense Miller) and B (L. barbarum L.). This result revealed that the pattern analysis of Lycii Fructus samples had a relationship with species (L. barbarum L. and L. chinense Miller) regardless of geographical origin (Fig. 4). Interestingly, although the contents of betaine were similar in the two species of L. barbarum L. and L. chinense Miller, Hclust could distinguish between L. barbarum L. and L. chinense Miller. Hence, this pattern analysis results could be used for the quality control of Lycii Fructus.Fig. 4

Bottom Line: Betaine was separated with an Atlantis hydrophilic interaction liquid chromatography silica column (4.6 × 150 mm, 5 μm, 100 Å) by isocratic elution using 30 mM ammonium acetate buffer and acetonitrile (20:80, v/v %) as the mobile phase.The method was fully validated with respect to linearity, precision, accuracy, stability and robustness.The HPLC/ELSD method was applied successfully to the quantification of betaine in the extract of Lycii Fructus.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, South Korea.

ABSTRACT
A rapid and simple high-performance liquid chromatography (HPLC) method with evaporative light scattering detection (ELSD) was developed for the determination of betaine from Lycii Fructus. Betaine was separated with an Atlantis hydrophilic interaction liquid chromatography silica column (4.6 × 150 mm, 5 μm, 100 Å) by isocratic elution using 30 mM ammonium acetate buffer and acetonitrile (20:80, v/v %) as the mobile phase. The flow rate was 1.0 mL/min, and the temperature for the spray chamber and drift tube was set at 30 and 50 °C, respectively. The method was fully validated with respect to linearity, precision, accuracy, stability and robustness. The HPLC/ELSD method was applied successfully to the quantification of betaine in the extract of Lycii Fructus. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of twenty-six L. barbarum L. from China (BC01-BC26), 3 L. barbarum L. (BJ27-BJ29) from Japan, 12 L. chinense Miller from China (CC30-CC41) and 51 L. chinense Miller samples (CK42-CK92) from Korea. The results indicate that the established HPLC/ELSD method is suitable for quality evaluation of Lycii Fructus.

Show MeSH
Related in: MedlinePlus