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Quantitative analysis of betaine in Lycii Fructus by HILIC-ELSD.

Zhao BT, Jeong SY, Hwangbo K, Moon DC, Seo EK, Lee D, Lee JH, Min BS, Ma ES, Son JK, Woo MH - Arch. Pharm. Res. (2013)

Bottom Line: Betaine was separated with an Atlantis hydrophilic interaction liquid chromatography silica column (4.6 × 150 mm, 5 μm, 100 Å) by isocratic elution using 30 mM ammonium acetate buffer and acetonitrile (20:80, v/v %) as the mobile phase.The method was fully validated with respect to linearity, precision, accuracy, stability and robustness.The HPLC/ELSD method was applied successfully to the quantification of betaine in the extract of Lycii Fructus.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, South Korea.

ABSTRACT
A rapid and simple high-performance liquid chromatography (HPLC) method with evaporative light scattering detection (ELSD) was developed for the determination of betaine from Lycii Fructus. Betaine was separated with an Atlantis hydrophilic interaction liquid chromatography silica column (4.6 × 150 mm, 5 μm, 100 Å) by isocratic elution using 30 mM ammonium acetate buffer and acetonitrile (20:80, v/v %) as the mobile phase. The flow rate was 1.0 mL/min, and the temperature for the spray chamber and drift tube was set at 30 and 50 °C, respectively. The method was fully validated with respect to linearity, precision, accuracy, stability and robustness. The HPLC/ELSD method was applied successfully to the quantification of betaine in the extract of Lycii Fructus. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of twenty-six L. barbarum L. from China (BC01-BC26), 3 L. barbarum L. (BJ27-BJ29) from Japan, 12 L. chinense Miller from China (CC30-CC41) and 51 L. chinense Miller samples (CK42-CK92) from Korea. The results indicate that the established HPLC/ELSD method is suitable for quality evaluation of Lycii Fructus.

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Structures of betaine and an internal standard
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Fig1: Structures of betaine and an internal standard

Mentions: The betaine standard was kindly provided by Professor Eun-Kyoung Seo (Ewha Womans University, Seoul, Korea), which was separated from L. chinense Miller (CK42). The internal standard (I.S.) of β-alanine was purchased from Junsei chemical. The compound structures are shown in Fig. 1. The purities of these compounds were determined to be greater than 98 % by normalization of the peak areas detected by HPLC analyses. Acetonitrile was purchased from Merck K GaA (Darmstadt, Germany). All other chemicals used were of analytic grade unless otherwise noted. Distilled water was prepared using the Milli-Q purification system (Millipore, Bedford, MA, USA). This study adopted the fruit samples of 26 L. barbarum L. from China (BC01–BC26), 3 L. barbarum L. (BJ27–BJ29) from Japan, 12 L. chinense Miller from China (CC30–CC41), 51 L. chinense Miller samples (CK42–CK92) were cultured from Korea. These samples cultured in different regions were provided by the National Center for Standardization of Herbal Medicine.Fig. 1


Quantitative analysis of betaine in Lycii Fructus by HILIC-ELSD.

Zhao BT, Jeong SY, Hwangbo K, Moon DC, Seo EK, Lee D, Lee JH, Min BS, Ma ES, Son JK, Woo MH - Arch. Pharm. Res. (2013)

Structures of betaine and an internal standard
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3824576&req=5

Fig1: Structures of betaine and an internal standard
Mentions: The betaine standard was kindly provided by Professor Eun-Kyoung Seo (Ewha Womans University, Seoul, Korea), which was separated from L. chinense Miller (CK42). The internal standard (I.S.) of β-alanine was purchased from Junsei chemical. The compound structures are shown in Fig. 1. The purities of these compounds were determined to be greater than 98 % by normalization of the peak areas detected by HPLC analyses. Acetonitrile was purchased from Merck K GaA (Darmstadt, Germany). All other chemicals used were of analytic grade unless otherwise noted. Distilled water was prepared using the Milli-Q purification system (Millipore, Bedford, MA, USA). This study adopted the fruit samples of 26 L. barbarum L. from China (BC01–BC26), 3 L. barbarum L. (BJ27–BJ29) from Japan, 12 L. chinense Miller from China (CC30–CC41), 51 L. chinense Miller samples (CK42–CK92) were cultured from Korea. These samples cultured in different regions were provided by the National Center for Standardization of Herbal Medicine.Fig. 1

Bottom Line: Betaine was separated with an Atlantis hydrophilic interaction liquid chromatography silica column (4.6 × 150 mm, 5 μm, 100 Å) by isocratic elution using 30 mM ammonium acetate buffer and acetonitrile (20:80, v/v %) as the mobile phase.The method was fully validated with respect to linearity, precision, accuracy, stability and robustness.The HPLC/ELSD method was applied successfully to the quantification of betaine in the extract of Lycii Fructus.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, South Korea.

ABSTRACT
A rapid and simple high-performance liquid chromatography (HPLC) method with evaporative light scattering detection (ELSD) was developed for the determination of betaine from Lycii Fructus. Betaine was separated with an Atlantis hydrophilic interaction liquid chromatography silica column (4.6 × 150 mm, 5 μm, 100 Å) by isocratic elution using 30 mM ammonium acetate buffer and acetonitrile (20:80, v/v %) as the mobile phase. The flow rate was 1.0 mL/min, and the temperature for the spray chamber and drift tube was set at 30 and 50 °C, respectively. The method was fully validated with respect to linearity, precision, accuracy, stability and robustness. The HPLC/ELSD method was applied successfully to the quantification of betaine in the extract of Lycii Fructus. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of twenty-six L. barbarum L. from China (BC01-BC26), 3 L. barbarum L. (BJ27-BJ29) from Japan, 12 L. chinense Miller from China (CC30-CC41) and 51 L. chinense Miller samples (CK42-CK92) from Korea. The results indicate that the established HPLC/ELSD method is suitable for quality evaluation of Lycii Fructus.

Show MeSH
Related in: MedlinePlus