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Mutational analysis of cj0183 Campylobacter jejuni promoter.

Salamasznska-Guz A, Grodzik M, Klimuszko D - Curr. Microbiol. (2013)

Bottom Line: It encodes a protein which shows partial homology to TlyC of Brachyspira hyodysenteriae.The aim of this work was to determine the mechanisms of gene regulation by cloning DNA fragments lying upstream of the cj0183 gene.Mutations in cj0183 -10 region, -16 region, and -35 region resulted in changes in gene transcription.

View Article: PubMed Central - PubMed

Affiliation: Department of Pre-Clinical Sciences, Warsaw University of Life Sciences, Warsaw, Poland, asalam@tlen.pl.

ABSTRACT
Gene-nominated cj0183 was identified in Campylobacter jejuni NCTC 11168 and in two human isolates 81116 and 81-176. It encodes a protein which shows partial homology to TlyC of Brachyspira hyodysenteriae. The aim of this work was to determine the mechanisms of gene regulation by cloning DNA fragments lying upstream of the cj0183 gene. The β-galactosidase activity determined for the strain harboring the plasmid with the fragment upstream of cj0183 indicated the presence of a promoter in this DNA region. Mutations in cj0183 -10 region, -16 region, and -35 region resulted in changes in gene transcription.

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Related in: MedlinePlus

Mapping of the transcriptional start site of cj0183 gene. Putative location of the cj0183 transcriptional start point determined by primer extension assay. Putative -35, -16, and -10 sequence motifs of a σ70-promoter and start codon of cj0183 are boxed. The ribosomal binding site is underlined
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Fig1: Mapping of the transcriptional start site of cj0183 gene. Putative location of the cj0183 transcriptional start point determined by primer extension assay. Putative -35, -16, and -10 sequence motifs of a σ70-promoter and start codon of cj0183 are boxed. The ribosomal binding site is underlined

Mentions: The coding and promoter sequences of the cj0183 gene are highly conserved among sequenced C. jejuni genomes (99–100 % for coding and 100 % for promoter sequence). Based on bioinformatic analysis (RSAT) it seems highly probable that cj0183 remains under the control of the σ70-recognized promoter [13]. Sequence of the upstream region of cj0183 showed no homology to sequences recognized by other sigma factors of C. jejuni. The cj0183 promoter contains three typical conserved promoter motifs: -10, -16, and -35 [17], at an appropriate distance upstream from the transcriptional start site (Fig. 1). Position of the first T in the -10 motif of the predicted promoter in relation to the start codon is 42 bp; majority of -10 motifs predicted by Petersen et al. [10] start 30–43 bp upstream of the start codon.Fig. 1


Mutational analysis of cj0183 Campylobacter jejuni promoter.

Salamasznska-Guz A, Grodzik M, Klimuszko D - Curr. Microbiol. (2013)

Mapping of the transcriptional start site of cj0183 gene. Putative location of the cj0183 transcriptional start point determined by primer extension assay. Putative -35, -16, and -10 sequence motifs of a σ70-promoter and start codon of cj0183 are boxed. The ribosomal binding site is underlined
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3824568&req=5

Fig1: Mapping of the transcriptional start site of cj0183 gene. Putative location of the cj0183 transcriptional start point determined by primer extension assay. Putative -35, -16, and -10 sequence motifs of a σ70-promoter and start codon of cj0183 are boxed. The ribosomal binding site is underlined
Mentions: The coding and promoter sequences of the cj0183 gene are highly conserved among sequenced C. jejuni genomes (99–100 % for coding and 100 % for promoter sequence). Based on bioinformatic analysis (RSAT) it seems highly probable that cj0183 remains under the control of the σ70-recognized promoter [13]. Sequence of the upstream region of cj0183 showed no homology to sequences recognized by other sigma factors of C. jejuni. The cj0183 promoter contains three typical conserved promoter motifs: -10, -16, and -35 [17], at an appropriate distance upstream from the transcriptional start site (Fig. 1). Position of the first T in the -10 motif of the predicted promoter in relation to the start codon is 42 bp; majority of -10 motifs predicted by Petersen et al. [10] start 30–43 bp upstream of the start codon.Fig. 1

Bottom Line: It encodes a protein which shows partial homology to TlyC of Brachyspira hyodysenteriae.The aim of this work was to determine the mechanisms of gene regulation by cloning DNA fragments lying upstream of the cj0183 gene.Mutations in cj0183 -10 region, -16 region, and -35 region resulted in changes in gene transcription.

View Article: PubMed Central - PubMed

Affiliation: Department of Pre-Clinical Sciences, Warsaw University of Life Sciences, Warsaw, Poland, asalam@tlen.pl.

ABSTRACT
Gene-nominated cj0183 was identified in Campylobacter jejuni NCTC 11168 and in two human isolates 81116 and 81-176. It encodes a protein which shows partial homology to TlyC of Brachyspira hyodysenteriae. The aim of this work was to determine the mechanisms of gene regulation by cloning DNA fragments lying upstream of the cj0183 gene. The β-galactosidase activity determined for the strain harboring the plasmid with the fragment upstream of cj0183 indicated the presence of a promoter in this DNA region. Mutations in cj0183 -10 region, -16 region, and -35 region resulted in changes in gene transcription.

Show MeSH
Related in: MedlinePlus