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Archaeal and bacterial communities in three alkaline hot springs in Heart Lake Geyser Basin, Yellowstone National Park.

Bowen De León K, Gerlach R, Peyton BM, Fields MW - Front Microbiol (2013)

Bottom Line: The archaeal community was more variable across time and was predominantly a methanogenic community in the 44 and 63°C springs and a thermophilic community in the 75°C spring.The 75°C spring demonstrated large shifts in the archaeal populations and was predominantly Candidatus Nitrosocaldus, an ammonia-oxidizing crenarchaeote, in the 2007 sample, and almost exclusively Thermofilum or Candidatus Caldiarchaeum in the 2009 sample, depending on SSU rRNA gene region examined.The majority of sequences were dissimilar (≥10% different) to any known organisms suggesting that HLGB possesses numerous new phylogenetic groups that warrant cultivation efforts.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Montana State University Bozeman, MT, USA ; Center for Biofilm Engineering, Montana State University Bozeman, MT, USA.

ABSTRACT
The Heart Lake Geyser Basin (HLGB) is remotely located at the base of Mount Sheridan in southern Yellowstone National Park (YNP), Wyoming, USA and is situated along Witch Creek and the northwestern shore of Heart Lake. Likely because of its location, little is known about the microbial community structure of springs in the HLGB. Bacterial and archaeal populations were monitored via small subunit (SSU) rRNA gene pyrosequencing over 3 years in 3 alkaline (pH 8.5) hot springs with varying temperatures (44°C, 63°C, 75°C). The bacterial populations were generally stable over time, but varied by temperature. The dominant bacterial community changed from moderately thermophilic and photosynthetic members (Cyanobacteria and Chloroflexi) at 44°C to a mixed photosynthetic and thermophilic community (Deinococcus-Thermus) at 63°C and a non-photosynthetic thermophilic community at 75°C. The archaeal community was more variable across time and was predominantly a methanogenic community in the 44 and 63°C springs and a thermophilic community in the 75°C spring. The 75°C spring demonstrated large shifts in the archaeal populations and was predominantly Candidatus Nitrosocaldus, an ammonia-oxidizing crenarchaeote, in the 2007 sample, and almost exclusively Thermofilum or Candidatus Caldiarchaeum in the 2009 sample, depending on SSU rRNA gene region examined. The majority of sequences were dissimilar (≥10% different) to any known organisms suggesting that HLGB possesses numerous new phylogenetic groups that warrant cultivation efforts.

No MeSH data available.


Related in: MedlinePlus

Difference from BLAST result for OTUs of varying relative abundances for the bacterial (A), archaeal forward (B), and archaeal reverse (C) datasets. The relative abundance of each OTU (clustered at 97% similarity) is plotted against the percent difference from the most similar BLAST result identified at the genus level.
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Figure 5: Difference from BLAST result for OTUs of varying relative abundances for the bacterial (A), archaeal forward (B), and archaeal reverse (C) datasets. The relative abundance of each OTU (clustered at 97% similarity) is plotted against the percent difference from the most similar BLAST result identified at the genus level.

Mentions: Many OTUs were different from any known organisms in the NCBI nucleotide database (Figure 5). The majority of OTUs with high similarity to a known organism were closely related to Deinococcus-Thermus and Cyanobacteria. Those at low relative abundance varied from 0 to 20% different from any known organisms, the point at which sequences were no longer considered as SSU rRNA gene sequences. The archaeal datasets (i.e., forward and reverse) had similar results in that relatively few OTUs were similar to anything in the database, even for those high in abundance.


Archaeal and bacterial communities in three alkaline hot springs in Heart Lake Geyser Basin, Yellowstone National Park.

Bowen De León K, Gerlach R, Peyton BM, Fields MW - Front Microbiol (2013)

Difference from BLAST result for OTUs of varying relative abundances for the bacterial (A), archaeal forward (B), and archaeal reverse (C) datasets. The relative abundance of each OTU (clustered at 97% similarity) is plotted against the percent difference from the most similar BLAST result identified at the genus level.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3824361&req=5

Figure 5: Difference from BLAST result for OTUs of varying relative abundances for the bacterial (A), archaeal forward (B), and archaeal reverse (C) datasets. The relative abundance of each OTU (clustered at 97% similarity) is plotted against the percent difference from the most similar BLAST result identified at the genus level.
Mentions: Many OTUs were different from any known organisms in the NCBI nucleotide database (Figure 5). The majority of OTUs with high similarity to a known organism were closely related to Deinococcus-Thermus and Cyanobacteria. Those at low relative abundance varied from 0 to 20% different from any known organisms, the point at which sequences were no longer considered as SSU rRNA gene sequences. The archaeal datasets (i.e., forward and reverse) had similar results in that relatively few OTUs were similar to anything in the database, even for those high in abundance.

Bottom Line: The archaeal community was more variable across time and was predominantly a methanogenic community in the 44 and 63°C springs and a thermophilic community in the 75°C spring.The 75°C spring demonstrated large shifts in the archaeal populations and was predominantly Candidatus Nitrosocaldus, an ammonia-oxidizing crenarchaeote, in the 2007 sample, and almost exclusively Thermofilum or Candidatus Caldiarchaeum in the 2009 sample, depending on SSU rRNA gene region examined.The majority of sequences were dissimilar (≥10% different) to any known organisms suggesting that HLGB possesses numerous new phylogenetic groups that warrant cultivation efforts.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Montana State University Bozeman, MT, USA ; Center for Biofilm Engineering, Montana State University Bozeman, MT, USA.

ABSTRACT
The Heart Lake Geyser Basin (HLGB) is remotely located at the base of Mount Sheridan in southern Yellowstone National Park (YNP), Wyoming, USA and is situated along Witch Creek and the northwestern shore of Heart Lake. Likely because of its location, little is known about the microbial community structure of springs in the HLGB. Bacterial and archaeal populations were monitored via small subunit (SSU) rRNA gene pyrosequencing over 3 years in 3 alkaline (pH 8.5) hot springs with varying temperatures (44°C, 63°C, 75°C). The bacterial populations were generally stable over time, but varied by temperature. The dominant bacterial community changed from moderately thermophilic and photosynthetic members (Cyanobacteria and Chloroflexi) at 44°C to a mixed photosynthetic and thermophilic community (Deinococcus-Thermus) at 63°C and a non-photosynthetic thermophilic community at 75°C. The archaeal community was more variable across time and was predominantly a methanogenic community in the 44 and 63°C springs and a thermophilic community in the 75°C spring. The 75°C spring demonstrated large shifts in the archaeal populations and was predominantly Candidatus Nitrosocaldus, an ammonia-oxidizing crenarchaeote, in the 2007 sample, and almost exclusively Thermofilum or Candidatus Caldiarchaeum in the 2009 sample, depending on SSU rRNA gene region examined. The majority of sequences were dissimilar (≥10% different) to any known organisms suggesting that HLGB possesses numerous new phylogenetic groups that warrant cultivation efforts.

No MeSH data available.


Related in: MedlinePlus