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Archaeal and bacterial communities in three alkaline hot springs in Heart Lake Geyser Basin, Yellowstone National Park.

Bowen De León K, Gerlach R, Peyton BM, Fields MW - Front Microbiol (2013)

Bottom Line: The archaeal community was more variable across time and was predominantly a methanogenic community in the 44 and 63°C springs and a thermophilic community in the 75°C spring.The 75°C spring demonstrated large shifts in the archaeal populations and was predominantly Candidatus Nitrosocaldus, an ammonia-oxidizing crenarchaeote, in the 2007 sample, and almost exclusively Thermofilum or Candidatus Caldiarchaeum in the 2009 sample, depending on SSU rRNA gene region examined.The majority of sequences were dissimilar (≥10% different) to any known organisms suggesting that HLGB possesses numerous new phylogenetic groups that warrant cultivation efforts.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Montana State University Bozeman, MT, USA ; Center for Biofilm Engineering, Montana State University Bozeman, MT, USA.

ABSTRACT
The Heart Lake Geyser Basin (HLGB) is remotely located at the base of Mount Sheridan in southern Yellowstone National Park (YNP), Wyoming, USA and is situated along Witch Creek and the northwestern shore of Heart Lake. Likely because of its location, little is known about the microbial community structure of springs in the HLGB. Bacterial and archaeal populations were monitored via small subunit (SSU) rRNA gene pyrosequencing over 3 years in 3 alkaline (pH 8.5) hot springs with varying temperatures (44°C, 63°C, 75°C). The bacterial populations were generally stable over time, but varied by temperature. The dominant bacterial community changed from moderately thermophilic and photosynthetic members (Cyanobacteria and Chloroflexi) at 44°C to a mixed photosynthetic and thermophilic community (Deinococcus-Thermus) at 63°C and a non-photosynthetic thermophilic community at 75°C. The archaeal community was more variable across time and was predominantly a methanogenic community in the 44 and 63°C springs and a thermophilic community in the 75°C spring. The 75°C spring demonstrated large shifts in the archaeal populations and was predominantly Candidatus Nitrosocaldus, an ammonia-oxidizing crenarchaeote, in the 2007 sample, and almost exclusively Thermofilum or Candidatus Caldiarchaeum in the 2009 sample, depending on SSU rRNA gene region examined. The majority of sequences were dissimilar (≥10% different) to any known organisms suggesting that HLGB possesses numerous new phylogenetic groups that warrant cultivation efforts.

No MeSH data available.


Related in: MedlinePlus

Relative abundance of bacterial phyla (A) and genera (B). Phyla (A) and genera (B) with a relative abundance <1 and <4%, respectively, were grouped as Other.
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Figure 2: Relative abundance of bacterial phyla (A) and genera (B). Phyla (A) and genera (B) with a relative abundance <1 and <4%, respectively, were grouped as Other.

Mentions: The bacterial populations were similar across time on the phylum and genus level for the 63 and 75°C spring samples, but differed in the 44°C spring samples (Figure 2). The 75°C spring was ~50% Thermus and the remaining 50% comprised of thermophilic genera. The 63°C spring was predominately Firmicutes and Chloroflexi. While 2007 and 2008 samples were also dominant in Cyanobacteria and Deinococcus-Thermus, the 2009 sample showed a decrease in these phyla and an increase in Dictyoglomi. On the genus level, the 2007 and 2008 samples for the 63°C spring differed in the relative abundance of Chloroflexus. The 2009 sample showed an increase in relative abundance of Dictyoglomus, Desulforudis, Thermovenabulum, and Verrucomicrobium compared to previous years. The 2007 and 2008 samples for the 44°C spring were similar on the phylum level and Chloroflexi were predominant (Figure 2). These samples were very diverse with genera <4% relative abundance comprising >50% of the total relative abundance. Levilinea was the genus with highest abundance and was only 5.6% of the total relative abundance. The 2008 sample showed a slight predominance of Oscillochloris and Syntrophus (14 and 10%, respectively) compared to other genera. The 2009 sample was less diverse and demonstrated a shift in dominance to Cyanobacteria, predominantly from the Pseudanabaena genus. Cluster analysis of the annual samples with the bacterial dataset resulted in distinct clustering by spring with the 75°C spring samples being quite dissimilar to samples from the other springs (Figure 4A).


Archaeal and bacterial communities in three alkaline hot springs in Heart Lake Geyser Basin, Yellowstone National Park.

Bowen De León K, Gerlach R, Peyton BM, Fields MW - Front Microbiol (2013)

Relative abundance of bacterial phyla (A) and genera (B). Phyla (A) and genera (B) with a relative abundance <1 and <4%, respectively, were grouped as Other.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3824361&req=5

Figure 2: Relative abundance of bacterial phyla (A) and genera (B). Phyla (A) and genera (B) with a relative abundance <1 and <4%, respectively, were grouped as Other.
Mentions: The bacterial populations were similar across time on the phylum and genus level for the 63 and 75°C spring samples, but differed in the 44°C spring samples (Figure 2). The 75°C spring was ~50% Thermus and the remaining 50% comprised of thermophilic genera. The 63°C spring was predominately Firmicutes and Chloroflexi. While 2007 and 2008 samples were also dominant in Cyanobacteria and Deinococcus-Thermus, the 2009 sample showed a decrease in these phyla and an increase in Dictyoglomi. On the genus level, the 2007 and 2008 samples for the 63°C spring differed in the relative abundance of Chloroflexus. The 2009 sample showed an increase in relative abundance of Dictyoglomus, Desulforudis, Thermovenabulum, and Verrucomicrobium compared to previous years. The 2007 and 2008 samples for the 44°C spring were similar on the phylum level and Chloroflexi were predominant (Figure 2). These samples were very diverse with genera <4% relative abundance comprising >50% of the total relative abundance. Levilinea was the genus with highest abundance and was only 5.6% of the total relative abundance. The 2008 sample showed a slight predominance of Oscillochloris and Syntrophus (14 and 10%, respectively) compared to other genera. The 2009 sample was less diverse and demonstrated a shift in dominance to Cyanobacteria, predominantly from the Pseudanabaena genus. Cluster analysis of the annual samples with the bacterial dataset resulted in distinct clustering by spring with the 75°C spring samples being quite dissimilar to samples from the other springs (Figure 4A).

Bottom Line: The archaeal community was more variable across time and was predominantly a methanogenic community in the 44 and 63°C springs and a thermophilic community in the 75°C spring.The 75°C spring demonstrated large shifts in the archaeal populations and was predominantly Candidatus Nitrosocaldus, an ammonia-oxidizing crenarchaeote, in the 2007 sample, and almost exclusively Thermofilum or Candidatus Caldiarchaeum in the 2009 sample, depending on SSU rRNA gene region examined.The majority of sequences were dissimilar (≥10% different) to any known organisms suggesting that HLGB possesses numerous new phylogenetic groups that warrant cultivation efforts.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Montana State University Bozeman, MT, USA ; Center for Biofilm Engineering, Montana State University Bozeman, MT, USA.

ABSTRACT
The Heart Lake Geyser Basin (HLGB) is remotely located at the base of Mount Sheridan in southern Yellowstone National Park (YNP), Wyoming, USA and is situated along Witch Creek and the northwestern shore of Heart Lake. Likely because of its location, little is known about the microbial community structure of springs in the HLGB. Bacterial and archaeal populations were monitored via small subunit (SSU) rRNA gene pyrosequencing over 3 years in 3 alkaline (pH 8.5) hot springs with varying temperatures (44°C, 63°C, 75°C). The bacterial populations were generally stable over time, but varied by temperature. The dominant bacterial community changed from moderately thermophilic and photosynthetic members (Cyanobacteria and Chloroflexi) at 44°C to a mixed photosynthetic and thermophilic community (Deinococcus-Thermus) at 63°C and a non-photosynthetic thermophilic community at 75°C. The archaeal community was more variable across time and was predominantly a methanogenic community in the 44 and 63°C springs and a thermophilic community in the 75°C spring. The 75°C spring demonstrated large shifts in the archaeal populations and was predominantly Candidatus Nitrosocaldus, an ammonia-oxidizing crenarchaeote, in the 2007 sample, and almost exclusively Thermofilum or Candidatus Caldiarchaeum in the 2009 sample, depending on SSU rRNA gene region examined. The majority of sequences were dissimilar (≥10% different) to any known organisms suggesting that HLGB possesses numerous new phylogenetic groups that warrant cultivation efforts.

No MeSH data available.


Related in: MedlinePlus