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Tissue kidney injury molecule-1 expression in the prediction of renal function for several years after kidney biopsy.

Simic Ogrizovic S, Bojic S, Basta-Jovanovic G, Radojevic S, Pavlovic J, Kotur Stevuljevic J, Dopsaj V, Naumovic R - Dis. Markers (2013)

Bottom Line: Kidney function (MDRD formula) and proteinuria/day were evaluated at the time of biopsy (GFR0) and 6, 12, 24, and 36 months later.Meanwhile, TIN inflammation was the best predictor of all measured kidney functions during three years, while tissue KIM-1 expression (P = 0.016) was a predictor only at 6 months after biopsy.Tissue KIM-1 expression significantly predicts kidney function solely at 6 months after biopsy, when the effects of immune and nonimmune treatments are the strongest.

View Article: PubMed Central - PubMed

Affiliation: Clinic of Nephrology, Clinical Center of Serbia, Pasterova 2, 11 000 Belgrade, Serbia ; School of Medicine, University of Belgrade, Serbia.

ABSTRACT

Objectives: Retrospective study was designed to examine the importance of tissue kidney injury molecule-1 (KIM-1) expression in predicting kidney function in sixty patients (27 males) aged 34.15 ± 12.23 years with different kidney diseases over three years after kidney biopsy.

Materials and methods: Tissue KIM-1 expression was determined immunohistochemically and KIM-1 staining was scored semiquantitatively, as well as tubulointerstitialis (TIN), inflammation, atrophy, and fibrosis. Kidney function (MDRD formula) and proteinuria/day were evaluated at the time of biopsy (GFR0) and 6, 12, 24, and 36 months later.

Results: Significantly positive correlations between tissue KIM-1 expression and age (r = 0.313), TIN inflammation (r = 0.456), fibrosis (r = 0.317), and proteinuria at 6 months (r = 0.394) as well as negative correlations with GFR0 (r = -0.572), GFR6 (r = -0.442), GFR24 (r = -0.398), and GFR36 (r = -0.412) were found. Meanwhile, TIN inflammation was the best predictor of all measured kidney functions during three years, while tissue KIM-1 expression (P = 0.016) was a predictor only at 6 months after biopsy.

Conclusion: Tissue KIM-1 expression significantly predicts kidney function solely at 6 months after biopsy, when the effects of immune and nonimmune treatments are the strongest.

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Related in: MedlinePlus

KIM-1 positive tubules are of intense red color. (a) Apical membrane of tubulocytes has the strongest affinity for KIM-1 specific antibodies. (b) Entire cytoplasm of tubulocytes has strong affinity for KIM-1 specific antibodies.
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fig1: KIM-1 positive tubules are of intense red color. (a) Apical membrane of tubulocytes has the strongest affinity for KIM-1 specific antibodies. (b) Entire cytoplasm of tubulocytes has strong affinity for KIM-1 specific antibodies.

Mentions: Tissue KIM-1 expression was significantly induced in all kidney biopsies except in one patient with MCGN. Tissue KIM-1 had a typically apical localization, but it could also be found in the cytoplasm with no apparent affinity for the apical membrane of tubulocytes. KIM-1 positive tubules were located mainly in the TIN regions affected by inflammation or fibrosis but were not observed in regions with end-stage fibrosis. KIM-1 positive tubules were often dilated cortical tubules, sometimes with initial signs of atrophy. Completely atrophic tubules were KIM-1 negative (Figure 1).


Tissue kidney injury molecule-1 expression in the prediction of renal function for several years after kidney biopsy.

Simic Ogrizovic S, Bojic S, Basta-Jovanovic G, Radojevic S, Pavlovic J, Kotur Stevuljevic J, Dopsaj V, Naumovic R - Dis. Markers (2013)

KIM-1 positive tubules are of intense red color. (a) Apical membrane of tubulocytes has the strongest affinity for KIM-1 specific antibodies. (b) Entire cytoplasm of tubulocytes has strong affinity for KIM-1 specific antibodies.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3824354&req=5

fig1: KIM-1 positive tubules are of intense red color. (a) Apical membrane of tubulocytes has the strongest affinity for KIM-1 specific antibodies. (b) Entire cytoplasm of tubulocytes has strong affinity for KIM-1 specific antibodies.
Mentions: Tissue KIM-1 expression was significantly induced in all kidney biopsies except in one patient with MCGN. Tissue KIM-1 had a typically apical localization, but it could also be found in the cytoplasm with no apparent affinity for the apical membrane of tubulocytes. KIM-1 positive tubules were located mainly in the TIN regions affected by inflammation or fibrosis but were not observed in regions with end-stage fibrosis. KIM-1 positive tubules were often dilated cortical tubules, sometimes with initial signs of atrophy. Completely atrophic tubules were KIM-1 negative (Figure 1).

Bottom Line: Kidney function (MDRD formula) and proteinuria/day were evaluated at the time of biopsy (GFR0) and 6, 12, 24, and 36 months later.Meanwhile, TIN inflammation was the best predictor of all measured kidney functions during three years, while tissue KIM-1 expression (P = 0.016) was a predictor only at 6 months after biopsy.Tissue KIM-1 expression significantly predicts kidney function solely at 6 months after biopsy, when the effects of immune and nonimmune treatments are the strongest.

View Article: PubMed Central - PubMed

Affiliation: Clinic of Nephrology, Clinical Center of Serbia, Pasterova 2, 11 000 Belgrade, Serbia ; School of Medicine, University of Belgrade, Serbia.

ABSTRACT

Objectives: Retrospective study was designed to examine the importance of tissue kidney injury molecule-1 (KIM-1) expression in predicting kidney function in sixty patients (27 males) aged 34.15 ± 12.23 years with different kidney diseases over three years after kidney biopsy.

Materials and methods: Tissue KIM-1 expression was determined immunohistochemically and KIM-1 staining was scored semiquantitatively, as well as tubulointerstitialis (TIN), inflammation, atrophy, and fibrosis. Kidney function (MDRD formula) and proteinuria/day were evaluated at the time of biopsy (GFR0) and 6, 12, 24, and 36 months later.

Results: Significantly positive correlations between tissue KIM-1 expression and age (r = 0.313), TIN inflammation (r = 0.456), fibrosis (r = 0.317), and proteinuria at 6 months (r = 0.394) as well as negative correlations with GFR0 (r = -0.572), GFR6 (r = -0.442), GFR24 (r = -0.398), and GFR36 (r = -0.412) were found. Meanwhile, TIN inflammation was the best predictor of all measured kidney functions during three years, while tissue KIM-1 expression (P = 0.016) was a predictor only at 6 months after biopsy.

Conclusion: Tissue KIM-1 expression significantly predicts kidney function solely at 6 months after biopsy, when the effects of immune and nonimmune treatments are the strongest.

Show MeSH
Related in: MedlinePlus