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Alterations in expression profile of iron-related genes in colorectal cancer.

Hamara K, Bielecka-Kowalska A, Przybylowska-Sygut K, Sygut A, Dziki A, Szemraj J - Mol. Biol. Rep. (2013)

Bottom Line: For the first time we have shown, that ferroportin concentration is significantly associated with miR-194 level, causing the reduction of this transporter amount in tumor tissues of patients with more advanced stages of CRC.We have also shown the alterations in expressing profile of miR-31, miR-133a, miR-141, miR-145, miR-149, miR-182 and miR-194, which were observed even in the early stage of disease, and identified a set of genes, which take place in correct assigning of patients in dependence of CRC stage.These iron-related genes could become potential diagnostic or prognostic indicators for patients with CRC.

View Article: PubMed Central - PubMed

ABSTRACT
Iron can play a role in colorectal cancer (CRC) development. The expression of genes involved in iron metabolism and its regulation in CRC has not been investigated well. Also the correlation between the level of iron-related genes expression and cancer progression is not known. In this study we collected paired samples of primary adenocarcinoma and adjacent normal mucosa from 73 patients. We assessed the mRNA or miRNA levels of 21 genes and verify their association with clinicopathological characteristics of CRC patients. Our experiments revealed, that the level of divalent metal transporter 1 transcript is well correlated with mRNA levels of iron regulatory proteins (IRPs) in tumor specimens. We have shown, that IRP2 can also be engaged in the mRNA stabilization of other iron transporter-transferrin receptor 1 (TfR1) in early stage of disease, however, in more advanced stages of CRC, mRNA level of TfR1 is related to miR-31 level. For the first time we have shown, that ferroportin concentration is significantly associated with miR-194 level, causing the reduction of this transporter amount in tumor tissues of patients with more advanced stages of CRC. We have also shown the alterations in expressing profile of miR-31, miR-133a, miR-141, miR-145, miR-149, miR-182 and miR-194, which were observed even in the early stage of disease, and identified a set of genes, which take place in correct assigning of patients in dependence of CRC stage. These iron-related genes could become potential diagnostic or prognostic indicators for patients with CRC.

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Expression of Heph gene in tumors localized in various parts of colon. Data presented as mean ± SE for the relative expression of analyzed gene calculated as . Significance of differences, estimated with one-way ANOVA and post hoc Tukey’s test was p = 0.040
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Fig2: Expression of Heph gene in tumors localized in various parts of colon. Data presented as mean ± SE for the relative expression of analyzed gene calculated as . Significance of differences, estimated with one-way ANOVA and post hoc Tukey’s test was p = 0.040

Mentions: According to QPCR data, the expression profiles of HJV, IRP2, TfR2 and miR-149 genes were dependent on depth of tumor invasion. HJV, IRP2 and TfR2 were up-regulated and mir-149 gene was down-regulated in T3 stage of CRC in comparison to T1 and T2 stages (Fig. 1). There was no statistically significant difference in the expression of remained iron-related genes among various T stages. Additionally, the Heph demonstrated higher expression in sigmoid colon than in cecum or rectum as was shown in Fig. 2, whereas the tumor location remained without significant influence for the expression of other tested genes. Moreover, we compared the expression of iron-related genes in male and female populations and found, that the Tf was significantly (p = 0.009) overexpressed in women. The data did not reveal any additional alterations in mRNA or miRNA levels in dependence on cancer cell differentiation, nodal invasion, diagnosis or patients age.Fig. 1


Alterations in expression profile of iron-related genes in colorectal cancer.

Hamara K, Bielecka-Kowalska A, Przybylowska-Sygut K, Sygut A, Dziki A, Szemraj J - Mol. Biol. Rep. (2013)

Expression of Heph gene in tumors localized in various parts of colon. Data presented as mean ± SE for the relative expression of analyzed gene calculated as . Significance of differences, estimated with one-way ANOVA and post hoc Tukey’s test was p = 0.040
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3824343&req=5

Fig2: Expression of Heph gene in tumors localized in various parts of colon. Data presented as mean ± SE for the relative expression of analyzed gene calculated as . Significance of differences, estimated with one-way ANOVA and post hoc Tukey’s test was p = 0.040
Mentions: According to QPCR data, the expression profiles of HJV, IRP2, TfR2 and miR-149 genes were dependent on depth of tumor invasion. HJV, IRP2 and TfR2 were up-regulated and mir-149 gene was down-regulated in T3 stage of CRC in comparison to T1 and T2 stages (Fig. 1). There was no statistically significant difference in the expression of remained iron-related genes among various T stages. Additionally, the Heph demonstrated higher expression in sigmoid colon than in cecum or rectum as was shown in Fig. 2, whereas the tumor location remained without significant influence for the expression of other tested genes. Moreover, we compared the expression of iron-related genes in male and female populations and found, that the Tf was significantly (p = 0.009) overexpressed in women. The data did not reveal any additional alterations in mRNA or miRNA levels in dependence on cancer cell differentiation, nodal invasion, diagnosis or patients age.Fig. 1

Bottom Line: For the first time we have shown, that ferroportin concentration is significantly associated with miR-194 level, causing the reduction of this transporter amount in tumor tissues of patients with more advanced stages of CRC.We have also shown the alterations in expressing profile of miR-31, miR-133a, miR-141, miR-145, miR-149, miR-182 and miR-194, which were observed even in the early stage of disease, and identified a set of genes, which take place in correct assigning of patients in dependence of CRC stage.These iron-related genes could become potential diagnostic or prognostic indicators for patients with CRC.

View Article: PubMed Central - PubMed

ABSTRACT
Iron can play a role in colorectal cancer (CRC) development. The expression of genes involved in iron metabolism and its regulation in CRC has not been investigated well. Also the correlation between the level of iron-related genes expression and cancer progression is not known. In this study we collected paired samples of primary adenocarcinoma and adjacent normal mucosa from 73 patients. We assessed the mRNA or miRNA levels of 21 genes and verify their association with clinicopathological characteristics of CRC patients. Our experiments revealed, that the level of divalent metal transporter 1 transcript is well correlated with mRNA levels of iron regulatory proteins (IRPs) in tumor specimens. We have shown, that IRP2 can also be engaged in the mRNA stabilization of other iron transporter-transferrin receptor 1 (TfR1) in early stage of disease, however, in more advanced stages of CRC, mRNA level of TfR1 is related to miR-31 level. For the first time we have shown, that ferroportin concentration is significantly associated with miR-194 level, causing the reduction of this transporter amount in tumor tissues of patients with more advanced stages of CRC. We have also shown the alterations in expressing profile of miR-31, miR-133a, miR-141, miR-145, miR-149, miR-182 and miR-194, which were observed even in the early stage of disease, and identified a set of genes, which take place in correct assigning of patients in dependence of CRC stage. These iron-related genes could become potential diagnostic or prognostic indicators for patients with CRC.

Show MeSH
Related in: MedlinePlus