Limits...
Resveratrol-loaded polymeric nanoparticles: validation of an HPLC-PDA method to determine the drug entrapment and evaluation of its antioxidant activity.

da Rocha Lindner G, Khalil NM, Mainardes RM - ScientificWorldJournal (2013)

Bottom Line: The mean diameter of the nanoparticles varied between 180 and 220 nm, and the encapsulation efficiency of RVT ranged from 60% to 88%.The profile obtained from the PLA nanoparticles containing RVT demonstrated that after 24 h, there was almost no increase in antioxidant activity, which was lower than that of the free RVT and RVT-loaded PLA-PEG nanoparticles.For PLA-PEG nanoparticles, the radical-scavenging activity of RVT was shown to increase with time, and after 48 h, it was similar to that observed with free RVT.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Laboratory of Pharmaceutical Nanotechnology, Universidade Estadual do Centro-Oeste/UNICENTRO, Rua Simeão Camargo Varela de Sá 03, 85040-080 Guarapuava, PR, Brazil.

ABSTRACT
Poly(lactic acid) (PLA) and PLA-poly(ethylene glycol) (PLA-PEG) nanoparticles containing resveratrol (RVT) were developed, and their antioxidant activity was evaluated. An analytical method using high performance liquid chromatography (HPLC)/photodiode array (PDA) detection was also developed and validated for RVT determination in nanoparticles. The mobile phase consisted of methanol : water (51 : 49, v/v) flowed at 0.9 mL/min, and the PDA detector was set at wavelength of 306 nm. The mean diameter of the nanoparticles varied between 180 and 220 nm, and the encapsulation efficiency of RVT ranged from 60% to 88%. The nanoparticles containing RVT were evaluated for their ability to scavenge the radical (2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) (ABTS•⁺). The profile obtained from the PLA nanoparticles containing RVT demonstrated that after 24 h, there was almost no increase in antioxidant activity, which was lower than that of the free RVT and RVT-loaded PLA-PEG nanoparticles. For PLA-PEG nanoparticles, the radical-scavenging activity of RVT was shown to increase with time, and after 48 h, it was similar to that observed with free RVT.

Show MeSH

Related in: MedlinePlus

Representative HPLC chromatograms of RVT sample in supernatant from nanoparticles (a) and supernatant from blank nanoparticles (b). Conditions: mobile phase, methanol : water (51 : 49, v/v); flow rate, 0.9 mL/min; PDA detection wavelength, 306 nm; column temperature, 25°C; injection volume, 20 μL.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3824335&req=5

fig2: Representative HPLC chromatograms of RVT sample in supernatant from nanoparticles (a) and supernatant from blank nanoparticles (b). Conditions: mobile phase, methanol : water (51 : 49, v/v); flow rate, 0.9 mL/min; PDA detection wavelength, 306 nm; column temperature, 25°C; injection volume, 20 μL.

Mentions: The specificity of the method was evaluated by comparing the chromatograms of RVT standards and samples to those with potential interfering formulation components. For this purpose, blank nanoparticles (drug-unloaded nanoparticles) were prepared as described in Section 2.6.1, and the supernatant obtained after centrifugation was diluted in a methanol : water mixture (50 : 50, v/v) and analyzed by the described HPLC method. The representative chromatogram of the RVT sample (Figure 2(a)) showed the RVT peak at approximately 6.4 min, which was in agreement with that obtained for the RVT standard (Figure 1). No peaks at this retention time were observed in the chromatogram of the supernatant from the blank nanoparticles (Figure 2(b)), which indicates that there was no interference in the quantitative determination of RVT from the formulation components.


Resveratrol-loaded polymeric nanoparticles: validation of an HPLC-PDA method to determine the drug entrapment and evaluation of its antioxidant activity.

da Rocha Lindner G, Khalil NM, Mainardes RM - ScientificWorldJournal (2013)

Representative HPLC chromatograms of RVT sample in supernatant from nanoparticles (a) and supernatant from blank nanoparticles (b). Conditions: mobile phase, methanol : water (51 : 49, v/v); flow rate, 0.9 mL/min; PDA detection wavelength, 306 nm; column temperature, 25°C; injection volume, 20 μL.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3824335&req=5

fig2: Representative HPLC chromatograms of RVT sample in supernatant from nanoparticles (a) and supernatant from blank nanoparticles (b). Conditions: mobile phase, methanol : water (51 : 49, v/v); flow rate, 0.9 mL/min; PDA detection wavelength, 306 nm; column temperature, 25°C; injection volume, 20 μL.
Mentions: The specificity of the method was evaluated by comparing the chromatograms of RVT standards and samples to those with potential interfering formulation components. For this purpose, blank nanoparticles (drug-unloaded nanoparticles) were prepared as described in Section 2.6.1, and the supernatant obtained after centrifugation was diluted in a methanol : water mixture (50 : 50, v/v) and analyzed by the described HPLC method. The representative chromatogram of the RVT sample (Figure 2(a)) showed the RVT peak at approximately 6.4 min, which was in agreement with that obtained for the RVT standard (Figure 1). No peaks at this retention time were observed in the chromatogram of the supernatant from the blank nanoparticles (Figure 2(b)), which indicates that there was no interference in the quantitative determination of RVT from the formulation components.

Bottom Line: The mean diameter of the nanoparticles varied between 180 and 220 nm, and the encapsulation efficiency of RVT ranged from 60% to 88%.The profile obtained from the PLA nanoparticles containing RVT demonstrated that after 24 h, there was almost no increase in antioxidant activity, which was lower than that of the free RVT and RVT-loaded PLA-PEG nanoparticles.For PLA-PEG nanoparticles, the radical-scavenging activity of RVT was shown to increase with time, and after 48 h, it was similar to that observed with free RVT.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Laboratory of Pharmaceutical Nanotechnology, Universidade Estadual do Centro-Oeste/UNICENTRO, Rua Simeão Camargo Varela de Sá 03, 85040-080 Guarapuava, PR, Brazil.

ABSTRACT
Poly(lactic acid) (PLA) and PLA-poly(ethylene glycol) (PLA-PEG) nanoparticles containing resveratrol (RVT) were developed, and their antioxidant activity was evaluated. An analytical method using high performance liquid chromatography (HPLC)/photodiode array (PDA) detection was also developed and validated for RVT determination in nanoparticles. The mobile phase consisted of methanol : water (51 : 49, v/v) flowed at 0.9 mL/min, and the PDA detector was set at wavelength of 306 nm. The mean diameter of the nanoparticles varied between 180 and 220 nm, and the encapsulation efficiency of RVT ranged from 60% to 88%. The nanoparticles containing RVT were evaluated for their ability to scavenge the radical (2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) (ABTS•⁺). The profile obtained from the PLA nanoparticles containing RVT demonstrated that after 24 h, there was almost no increase in antioxidant activity, which was lower than that of the free RVT and RVT-loaded PLA-PEG nanoparticles. For PLA-PEG nanoparticles, the radical-scavenging activity of RVT was shown to increase with time, and after 48 h, it was similar to that observed with free RVT.

Show MeSH
Related in: MedlinePlus