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Applications of fluorescent sensor based on 1H-pyrazolo[3,4-b]quinoline in analytical chemistry.

Mac M, Uchacz T, Danel A, Musiolik H - J Fluoresc (2013)

Bottom Line: Relatively high sensitivity but poor selectivity of the amino alcohol that contains indicator towards the two-valued cations was observed.However, upon addition of some amounts of water the selectivity of this sensor has been enhanced (especially towards lead cation).The preliminary results in analytical application of the sensor are discussed.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060, Krakow, Poland.

ABSTRACT
Fluorescent dye 2-[(2-Hydroxyethyl)-(1,3-diphenyl-1H-pyrazolo[3,4-b]quinolin-6-ylmethyl)-amino]ethanol (LL1) was examined for its efficiency in the detection of small inorganic cations (lithium, sodium, barium, calcium, magnesium, cadmium, lead and zinc). The dye was synthesized in the laboratory and investigated by means of both, steady-state and time-resolved fluorescence techniques. This compound acts as a fluorescent sensor suitable for detection of small inorganic cations (lithium, sodium, barium, calcium, magnesium, cadmium, lead and zinc) in strongly polar solvent (acetonitrile). An electron transfer from the electro-donative part (receptor) of the molecule to the acceptor part (fluorophore) is thought to be the main mechanism that underlies functionality of the compound as a sensor. This process can be retarded upon complexation of the receptor moiety by inorganic cations. Relatively high sensitivity but poor selectivity of the amino alcohol that contains indicator towards the two-valued cations was observed. However, upon addition of some amounts of water the selectivity of this sensor has been enhanced (especially towards lead cation). The preliminary results in analytical application of the sensor are discussed.

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Room absorption and fluorescence (inset) spectra of 1,3-diphenyl-1H-pyrazolo[3,4-b]quinoline (solid), LL1 (dash), LL1+Mg(ClO4)2 (dash dot dot) in acetonitrile. The concentration of Mg(ClO4)2 is equal to 4.104 M
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Fig1: Room absorption and fluorescence (inset) spectra of 1,3-diphenyl-1H-pyrazolo[3,4-b]quinoline (solid), LL1 (dash), LL1+Mg(ClO4)2 (dash dot dot) in acetonitrile. The concentration of Mg(ClO4)2 is equal to 4.104 M

Mentions: The absorption spectrum of LL1 in acetonitrile consists of two separate bands located between 250 and 325 nm and above 350 nm, respectively (Fig. 1). The first one is a structured band with a maximum at 260 nm (ε = 57400 dm3mol−1cm−1) while the second one is a Gaussian-like band having maximum at 397 nm (molar absorption coefficient ε = 7400 dm3mol−1cm−1). A simple comparison of the absorption spectra of LL1 and the parent molecule 1,3-diphenyl-1H-pyrazolo[3,4-b]quinoline (fluorophore of LL1) reveals a negligible effect of the electron-donating macrocyclic recognition unit on the energetics of the optical transitions within the fluorophore. For instance, the maxima of the absorption and fluorescence bands of LL1 in acetonitrile were found at 397 nm and 481 nm, whilst those of the fluorophore are centred at 395 nm 476 nm (see Fig. 1). It can also be inferred that the absorption spectra of LL1 are rather insensitive to the solvent polarity.Fig. 1


Applications of fluorescent sensor based on 1H-pyrazolo[3,4-b]quinoline in analytical chemistry.

Mac M, Uchacz T, Danel A, Musiolik H - J Fluoresc (2013)

Room absorption and fluorescence (inset) spectra of 1,3-diphenyl-1H-pyrazolo[3,4-b]quinoline (solid), LL1 (dash), LL1+Mg(ClO4)2 (dash dot dot) in acetonitrile. The concentration of Mg(ClO4)2 is equal to 4.104 M
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3824276&req=5

Fig1: Room absorption and fluorescence (inset) spectra of 1,3-diphenyl-1H-pyrazolo[3,4-b]quinoline (solid), LL1 (dash), LL1+Mg(ClO4)2 (dash dot dot) in acetonitrile. The concentration of Mg(ClO4)2 is equal to 4.104 M
Mentions: The absorption spectrum of LL1 in acetonitrile consists of two separate bands located between 250 and 325 nm and above 350 nm, respectively (Fig. 1). The first one is a structured band with a maximum at 260 nm (ε = 57400 dm3mol−1cm−1) while the second one is a Gaussian-like band having maximum at 397 nm (molar absorption coefficient ε = 7400 dm3mol−1cm−1). A simple comparison of the absorption spectra of LL1 and the parent molecule 1,3-diphenyl-1H-pyrazolo[3,4-b]quinoline (fluorophore of LL1) reveals a negligible effect of the electron-donating macrocyclic recognition unit on the energetics of the optical transitions within the fluorophore. For instance, the maxima of the absorption and fluorescence bands of LL1 in acetonitrile were found at 397 nm and 481 nm, whilst those of the fluorophore are centred at 395 nm 476 nm (see Fig. 1). It can also be inferred that the absorption spectra of LL1 are rather insensitive to the solvent polarity.Fig. 1

Bottom Line: Relatively high sensitivity but poor selectivity of the amino alcohol that contains indicator towards the two-valued cations was observed.However, upon addition of some amounts of water the selectivity of this sensor has been enhanced (especially towards lead cation).The preliminary results in analytical application of the sensor are discussed.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060, Krakow, Poland.

ABSTRACT
Fluorescent dye 2-[(2-Hydroxyethyl)-(1,3-diphenyl-1H-pyrazolo[3,4-b]quinolin-6-ylmethyl)-amino]ethanol (LL1) was examined for its efficiency in the detection of small inorganic cations (lithium, sodium, barium, calcium, magnesium, cadmium, lead and zinc). The dye was synthesized in the laboratory and investigated by means of both, steady-state and time-resolved fluorescence techniques. This compound acts as a fluorescent sensor suitable for detection of small inorganic cations (lithium, sodium, barium, calcium, magnesium, cadmium, lead and zinc) in strongly polar solvent (acetonitrile). An electron transfer from the electro-donative part (receptor) of the molecule to the acceptor part (fluorophore) is thought to be the main mechanism that underlies functionality of the compound as a sensor. This process can be retarded upon complexation of the receptor moiety by inorganic cations. Relatively high sensitivity but poor selectivity of the amino alcohol that contains indicator towards the two-valued cations was observed. However, upon addition of some amounts of water the selectivity of this sensor has been enhanced (especially towards lead cation). The preliminary results in analytical application of the sensor are discussed.

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