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High density of peritumoral lymphatic vessels measured by D2-40/podoplanin and LYVE-1 expression in gastric cancer patients: an excellent prognostic indicator or a false friend?

Rudno-Rudzinska J, Kielan W, Grzebieniak Z, Dziegiel P, Donizy P, Mazur G, Knakiewicz M, Frejlich E, Halon A - Gastric Cancer (2012)

Bottom Line: Statistical analysis showed no impact of increased intratumoral or peritumoral LVD on gastric cancer patient survival, irrespective of the protein used to stain lymphatic vessels.Analysis showed that the probability of overall survival was decreased in the cases with enhanced VEGF-D immunoreactivity (P = 0.0045).VEGF-D is the only marker that can be regarded as an unfavorable prognostic indicator for patients with advanced gastric cancer.

View Article: PubMed Central - PubMed

Affiliation: 2nd Department of General and Oncological Surgery, Wroclaw Medical University, Borowska 213, 50-556, Wrocław, Poland, jrr@id.pl.

ABSTRACT

Background: One of the most important prognostic indicators in gastric cancer is the presence of metastases in lymph nodes. Even now, little is known about lymphangiogenesis in neoplastic tissue, and little is also known about the transmission of a neoplastic cell from the tumor mass into a lymphatic vessel.

Methods: This study examined the relationships between the density of lymphatic vessels (LVD) stained immunohistochemically with lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) and D2-40 (podoplanin) antibodies, the expression of vascular endothelial growth factor (VEGF)-C/D, selected clinical and pathomorphological factors, and the 5-year overall survival of gastric cancer patients.

Results: Statistical analysis showed no impact of increased intratumoral or peritumoral LVD on gastric cancer patient survival, irrespective of the protein used to stain lymphatic vessels. Analysis showed that the probability of overall survival was decreased in the cases with enhanced VEGF-D immunoreactivity (P = 0.0045).

Conclusion: The study showed that the studied markers cannot be used to determine the required extent of the surgical procedure, as they have no statistically significant correlation with the degree of progression of the cancer, the stage of the disease assessed according to the TNM 5th classification of malignant tumors, clinicopathological features, and patient survival. VEGF-D is the only marker that can be regarded as an unfavorable prognostic indicator for patients with advanced gastric cancer.

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Comparison of lymphatic vessels staining with two different markers in pairs in the same case. Intratumoral expression of LYVE-1 (a), D2-40 (b), vascular endothelial growth factors (VEGF-C) (c), and VEGF-D (d). a, b ×200; c, d ×100
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Fig2: Comparison of lymphatic vessels staining with two different markers in pairs in the same case. Intratumoral expression of LYVE-1 (a), D2-40 (b), vascular endothelial growth factors (VEGF-C) (c), and VEGF-D (d). a, b ×200; c, d ×100

Mentions: Comparison of the densities of lymphatic vessels stained with the two markers demonstrated that both the mean and maximum LVD were higher if D2-40 had been used (Fig. 2a, b). Analysis revealed that the difference was statistically significant both in the intratumoral compartment and in the peritumoral zone (Wilcoxon test: Z = 4.9, P = 0.000001; Z = 2.28, P = 0.02, respectively).Fig. 2


High density of peritumoral lymphatic vessels measured by D2-40/podoplanin and LYVE-1 expression in gastric cancer patients: an excellent prognostic indicator or a false friend?

Rudno-Rudzinska J, Kielan W, Grzebieniak Z, Dziegiel P, Donizy P, Mazur G, Knakiewicz M, Frejlich E, Halon A - Gastric Cancer (2012)

Comparison of lymphatic vessels staining with two different markers in pairs in the same case. Intratumoral expression of LYVE-1 (a), D2-40 (b), vascular endothelial growth factors (VEGF-C) (c), and VEGF-D (d). a, b ×200; c, d ×100
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3824260&req=5

Fig2: Comparison of lymphatic vessels staining with two different markers in pairs in the same case. Intratumoral expression of LYVE-1 (a), D2-40 (b), vascular endothelial growth factors (VEGF-C) (c), and VEGF-D (d). a, b ×200; c, d ×100
Mentions: Comparison of the densities of lymphatic vessels stained with the two markers demonstrated that both the mean and maximum LVD were higher if D2-40 had been used (Fig. 2a, b). Analysis revealed that the difference was statistically significant both in the intratumoral compartment and in the peritumoral zone (Wilcoxon test: Z = 4.9, P = 0.000001; Z = 2.28, P = 0.02, respectively).Fig. 2

Bottom Line: Statistical analysis showed no impact of increased intratumoral or peritumoral LVD on gastric cancer patient survival, irrespective of the protein used to stain lymphatic vessels.Analysis showed that the probability of overall survival was decreased in the cases with enhanced VEGF-D immunoreactivity (P = 0.0045).VEGF-D is the only marker that can be regarded as an unfavorable prognostic indicator for patients with advanced gastric cancer.

View Article: PubMed Central - PubMed

Affiliation: 2nd Department of General and Oncological Surgery, Wroclaw Medical University, Borowska 213, 50-556, Wrocław, Poland, jrr@id.pl.

ABSTRACT

Background: One of the most important prognostic indicators in gastric cancer is the presence of metastases in lymph nodes. Even now, little is known about lymphangiogenesis in neoplastic tissue, and little is also known about the transmission of a neoplastic cell from the tumor mass into a lymphatic vessel.

Methods: This study examined the relationships between the density of lymphatic vessels (LVD) stained immunohistochemically with lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) and D2-40 (podoplanin) antibodies, the expression of vascular endothelial growth factor (VEGF)-C/D, selected clinical and pathomorphological factors, and the 5-year overall survival of gastric cancer patients.

Results: Statistical analysis showed no impact of increased intratumoral or peritumoral LVD on gastric cancer patient survival, irrespective of the protein used to stain lymphatic vessels. Analysis showed that the probability of overall survival was decreased in the cases with enhanced VEGF-D immunoreactivity (P = 0.0045).

Conclusion: The study showed that the studied markers cannot be used to determine the required extent of the surgical procedure, as they have no statistically significant correlation with the degree of progression of the cancer, the stage of the disease assessed according to the TNM 5th classification of malignant tumors, clinicopathological features, and patient survival. VEGF-D is the only marker that can be regarded as an unfavorable prognostic indicator for patients with advanced gastric cancer.

Show MeSH
Related in: MedlinePlus