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Deoxynivalenol affects the composition of the basement membrane proteins and influences en route the migration of CD16(+) cells into the intestinal epithelium.

Nossol C, Diesing AK, Kahlert S, Kersten S, Kluess J, Ponsuksili S, Hartig R, Wimmers K, Dänicke S, Rothkötter HJ - Mycotoxin Res (2013)

Bottom Line: The in vivo results were extended with microarray analyses of epithelial cell (IPEC-J2 cells).Our results provide evidence that already low basolateral concentrations of DON (50 ng/mL) influence the production of the BM protein laminin by epithelial cells.Thus, DON affects the composition of the BM.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anatomy, Otto-von-Guericke University Magdeburg, Leipziger Strasse 44, 39120, Magdeburg, Germany, constanze.nossol@med.ovgu.de.

ABSTRACT
The numerous pores in the basement membrane (BM) of the intestinal villi are essential for the communication of enterocytes with cells in the lamina propria, an important mechanism for the induction of intestinal immune responses. The intestinal epithelial barrier is affected by the mycotoxin deoxynivalenol (DON) from both the apical (luminal) and basolateral (serosal) side. The pig is the most susceptible species to the anorectic and immune-modulating effects of DON, which is most prevalent in crops. We analysed in pigs the effect of DON-contaminated feed on the composition and perforation of the BM and the presence of CD16(+) cells or their dendrites in the epithelium. In addition to in vivo experiments, in vitro studies were carried out. Using microarray analyses, the effects of DON on IPEC-J2 cells were studied with the focus on the BM. Our in vivo results showed in the control pigs: (1) a significant increased pore number (p ≤ 0.001) in the jejunum in comparison to ileum, (2) no difference in the pore size, and (3) comparable frequency of intraepithelial CD16(+) cells/dendrites in the jejunum and ileum. There was a marked trend that DON feeding increases: (1) the pore number in jejunum, and (2) the number of CD16(+) cells/dendrites in the epithelium (Tukey-Kramer; p = 0.055 and p = 0.067, respectively). The in vivo results were extended with microarray analyses of epithelial cell (IPEC-J2 cells). The down-regulation of genes like syndecan, fibulin 6 and BM-40 was observed. These proteins are important factors in the BM composition and in formation of pores. Our results provide evidence that already low basolateral concentrations of DON (50 ng/mL) influence the production of the BM protein laminin by epithelial cells. Thus, DON affects the composition of the BM.

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Average number of CD16+ cells or dendrites. No differences were found between the groups (F test; p = 0.509). A marked effect was observed with focus on gut segment (T; p = 0.087) and the interaction of group × gut segment (T; p = 0.091). A higher number of CD16+ cells/dendrites were detected in the DON-diet group of the jejunum in comparison to control (control: 3.4; DON group: 4.9). The comparison of the control and DON-diet group of the ileum resulted in no significant differences. A tendency of a higher number of CD16+ cell/dendrites were found in the comparison of the two DON-diet groups (t test; T, p = 0.067)
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Fig6: Average number of CD16+ cells or dendrites. No differences were found between the groups (F test; p = 0.509). A marked effect was observed with focus on gut segment (T; p = 0.087) and the interaction of group × gut segment (T; p = 0.091). A higher number of CD16+ cells/dendrites were detected in the DON-diet group of the jejunum in comparison to control (control: 3.4; DON group: 4.9). The comparison of the control and DON-diet group of the ileum resulted in no significant differences. A tendency of a higher number of CD16+ cell/dendrites were found in the comparison of the two DON-diet groups (t test; T, p = 0.067)

Mentions: The frequency of CD16+ cells or dendrites of CD16+ cells/1,000 μm BM was determined. In the first statistical analysis, the dataset was characterised using a two-way design to detect any effect of the intestinal segment and treatment. No differences were found between the treatments (F test, p = 0.509). A marked effect was observed between the intestinal segments (trend; p = 0.087) and the interaction of ‘treatment × intestinal segment’ (trend; p = 0.091). In the next step, the least square mean differences were analysed with a Tukey–Kramer post hoc test. A tendency of an up-regulation of CD16+ cells/dendrites was found in the DON-treatment group of the jejunum in comparison to the DON-group of the ileum (jejunum: 4.9; ileum: 2.8; p = 0.067; Fig. 6). No differences between control and DON-diet groups were observed in the ileum. The comparison of both control groups showed no significant differences.Fig. 6


Deoxynivalenol affects the composition of the basement membrane proteins and influences en route the migration of CD16(+) cells into the intestinal epithelium.

Nossol C, Diesing AK, Kahlert S, Kersten S, Kluess J, Ponsuksili S, Hartig R, Wimmers K, Dänicke S, Rothkötter HJ - Mycotoxin Res (2013)

Average number of CD16+ cells or dendrites. No differences were found between the groups (F test; p = 0.509). A marked effect was observed with focus on gut segment (T; p = 0.087) and the interaction of group × gut segment (T; p = 0.091). A higher number of CD16+ cells/dendrites were detected in the DON-diet group of the jejunum in comparison to control (control: 3.4; DON group: 4.9). The comparison of the control and DON-diet group of the ileum resulted in no significant differences. A tendency of a higher number of CD16+ cell/dendrites were found in the comparison of the two DON-diet groups (t test; T, p = 0.067)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3824239&req=5

Fig6: Average number of CD16+ cells or dendrites. No differences were found between the groups (F test; p = 0.509). A marked effect was observed with focus on gut segment (T; p = 0.087) and the interaction of group × gut segment (T; p = 0.091). A higher number of CD16+ cells/dendrites were detected in the DON-diet group of the jejunum in comparison to control (control: 3.4; DON group: 4.9). The comparison of the control and DON-diet group of the ileum resulted in no significant differences. A tendency of a higher number of CD16+ cell/dendrites were found in the comparison of the two DON-diet groups (t test; T, p = 0.067)
Mentions: The frequency of CD16+ cells or dendrites of CD16+ cells/1,000 μm BM was determined. In the first statistical analysis, the dataset was characterised using a two-way design to detect any effect of the intestinal segment and treatment. No differences were found between the treatments (F test, p = 0.509). A marked effect was observed between the intestinal segments (trend; p = 0.087) and the interaction of ‘treatment × intestinal segment’ (trend; p = 0.091). In the next step, the least square mean differences were analysed with a Tukey–Kramer post hoc test. A tendency of an up-regulation of CD16+ cells/dendrites was found in the DON-treatment group of the jejunum in comparison to the DON-group of the ileum (jejunum: 4.9; ileum: 2.8; p = 0.067; Fig. 6). No differences between control and DON-diet groups were observed in the ileum. The comparison of both control groups showed no significant differences.Fig. 6

Bottom Line: The in vivo results were extended with microarray analyses of epithelial cell (IPEC-J2 cells).Our results provide evidence that already low basolateral concentrations of DON (50 ng/mL) influence the production of the BM protein laminin by epithelial cells.Thus, DON affects the composition of the BM.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anatomy, Otto-von-Guericke University Magdeburg, Leipziger Strasse 44, 39120, Magdeburg, Germany, constanze.nossol@med.ovgu.de.

ABSTRACT
The numerous pores in the basement membrane (BM) of the intestinal villi are essential for the communication of enterocytes with cells in the lamina propria, an important mechanism for the induction of intestinal immune responses. The intestinal epithelial barrier is affected by the mycotoxin deoxynivalenol (DON) from both the apical (luminal) and basolateral (serosal) side. The pig is the most susceptible species to the anorectic and immune-modulating effects of DON, which is most prevalent in crops. We analysed in pigs the effect of DON-contaminated feed on the composition and perforation of the BM and the presence of CD16(+) cells or their dendrites in the epithelium. In addition to in vivo experiments, in vitro studies were carried out. Using microarray analyses, the effects of DON on IPEC-J2 cells were studied with the focus on the BM. Our in vivo results showed in the control pigs: (1) a significant increased pore number (p ≤ 0.001) in the jejunum in comparison to ileum, (2) no difference in the pore size, and (3) comparable frequency of intraepithelial CD16(+) cells/dendrites in the jejunum and ileum. There was a marked trend that DON feeding increases: (1) the pore number in jejunum, and (2) the number of CD16(+) cells/dendrites in the epithelium (Tukey-Kramer; p = 0.055 and p = 0.067, respectively). The in vivo results were extended with microarray analyses of epithelial cell (IPEC-J2 cells). The down-regulation of genes like syndecan, fibulin 6 and BM-40 was observed. These proteins are important factors in the BM composition and in formation of pores. Our results provide evidence that already low basolateral concentrations of DON (50 ng/mL) influence the production of the BM protein laminin by epithelial cells. Thus, DON affects the composition of the BM.

Show MeSH
Related in: MedlinePlus