Limits...
Deoxynivalenol affects the composition of the basement membrane proteins and influences en route the migration of CD16(+) cells into the intestinal epithelium.

Nossol C, Diesing AK, Kahlert S, Kersten S, Kluess J, Ponsuksili S, Hartig R, Wimmers K, Dänicke S, Rothkötter HJ - Mycotoxin Res (2013)

Bottom Line: The in vivo results were extended with microarray analyses of epithelial cell (IPEC-J2 cells).Our results provide evidence that already low basolateral concentrations of DON (50 ng/mL) influence the production of the BM protein laminin by epithelial cells.Thus, DON affects the composition of the BM.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anatomy, Otto-von-Guericke University Magdeburg, Leipziger Strasse 44, 39120, Magdeburg, Germany, constanze.nossol@med.ovgu.de.

ABSTRACT
The numerous pores in the basement membrane (BM) of the intestinal villi are essential for the communication of enterocytes with cells in the lamina propria, an important mechanism for the induction of intestinal immune responses. The intestinal epithelial barrier is affected by the mycotoxin deoxynivalenol (DON) from both the apical (luminal) and basolateral (serosal) side. The pig is the most susceptible species to the anorectic and immune-modulating effects of DON, which is most prevalent in crops. We analysed in pigs the effect of DON-contaminated feed on the composition and perforation of the BM and the presence of CD16(+) cells or their dendrites in the epithelium. In addition to in vivo experiments, in vitro studies were carried out. Using microarray analyses, the effects of DON on IPEC-J2 cells were studied with the focus on the BM. Our in vivo results showed in the control pigs: (1) a significant increased pore number (p ≤ 0.001) in the jejunum in comparison to ileum, (2) no difference in the pore size, and (3) comparable frequency of intraepithelial CD16(+) cells/dendrites in the jejunum and ileum. There was a marked trend that DON feeding increases: (1) the pore number in jejunum, and (2) the number of CD16(+) cells/dendrites in the epithelium (Tukey-Kramer; p = 0.055 and p = 0.067, respectively). The in vivo results were extended with microarray analyses of epithelial cell (IPEC-J2 cells). The down-regulation of genes like syndecan, fibulin 6 and BM-40 was observed. These proteins are important factors in the BM composition and in formation of pores. Our results provide evidence that already low basolateral concentrations of DON (50 ng/mL) influence the production of the BM protein laminin by epithelial cells. Thus, DON affects the composition of the BM.

Show MeSH

Related in: MedlinePlus

Distribution of the pore size. In the control groups of the jejunum and the ileum, a total amount of 498 (jejunum) and 931 pores (ileum) were analysed. The percentage of the different pore sizes is illustrated. In the jejunum as well as in the ileum, about 80 % of the pores had a size between 0.25 and 5 μm. In the DON-diet groups, a total amount of 788 (jejunum) and 1,035 (ileum) pores were found; 74–76 % of the pores had a size between 0.11 and 0.5 μm
© Copyright Policy - OpenAccess
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3824239&req=5

Fig2: Distribution of the pore size. In the control groups of the jejunum and the ileum, a total amount of 498 (jejunum) and 931 pores (ileum) were analysed. The percentage of the different pore sizes is illustrated. In the jejunum as well as in the ileum, about 80 % of the pores had a size between 0.25 and 5 μm. In the DON-diet groups, a total amount of 788 (jejunum) and 1,035 (ileum) pores were found; 74–76 % of the pores had a size between 0.11 and 0.5 μm

Mentions: The presence of laminin was used as a marker to localise the BM in 5-μm cryo-sections in the jejunum and ileum. Analysis of BM pores in jejunum and ileum of non-treated control animals resulted in pore sizes ranging from 0.51 to 27.95 μm in the jejunum and from 0.25 to 34.26 μm in the ileum. Pores were clustered in size ranges spanning 1 μm and numbers of pores within each cluster were plotted (Fig. 2). In both segments of the intestine, large pores greater than 10 μm are rare. About 80 % of the pores were smaller than 5 μm. An average pore size of 3.6 μm was measured in the jejunum and of 3.4 μm in the ileum (Fig. 3). Similar results were found for the DON-diet group (jejunum: 3.8 μm; ileum: 4 μm). The average pore size was independent of the intestinal segment and independent of DON feeding.Fig. 2


Deoxynivalenol affects the composition of the basement membrane proteins and influences en route the migration of CD16(+) cells into the intestinal epithelium.

Nossol C, Diesing AK, Kahlert S, Kersten S, Kluess J, Ponsuksili S, Hartig R, Wimmers K, Dänicke S, Rothkötter HJ - Mycotoxin Res (2013)

Distribution of the pore size. In the control groups of the jejunum and the ileum, a total amount of 498 (jejunum) and 931 pores (ileum) were analysed. The percentage of the different pore sizes is illustrated. In the jejunum as well as in the ileum, about 80 % of the pores had a size between 0.25 and 5 μm. In the DON-diet groups, a total amount of 788 (jejunum) and 1,035 (ileum) pores were found; 74–76 % of the pores had a size between 0.11 and 0.5 μm
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3824239&req=5

Fig2: Distribution of the pore size. In the control groups of the jejunum and the ileum, a total amount of 498 (jejunum) and 931 pores (ileum) were analysed. The percentage of the different pore sizes is illustrated. In the jejunum as well as in the ileum, about 80 % of the pores had a size between 0.25 and 5 μm. In the DON-diet groups, a total amount of 788 (jejunum) and 1,035 (ileum) pores were found; 74–76 % of the pores had a size between 0.11 and 0.5 μm
Mentions: The presence of laminin was used as a marker to localise the BM in 5-μm cryo-sections in the jejunum and ileum. Analysis of BM pores in jejunum and ileum of non-treated control animals resulted in pore sizes ranging from 0.51 to 27.95 μm in the jejunum and from 0.25 to 34.26 μm in the ileum. Pores were clustered in size ranges spanning 1 μm and numbers of pores within each cluster were plotted (Fig. 2). In both segments of the intestine, large pores greater than 10 μm are rare. About 80 % of the pores were smaller than 5 μm. An average pore size of 3.6 μm was measured in the jejunum and of 3.4 μm in the ileum (Fig. 3). Similar results were found for the DON-diet group (jejunum: 3.8 μm; ileum: 4 μm). The average pore size was independent of the intestinal segment and independent of DON feeding.Fig. 2

Bottom Line: The in vivo results were extended with microarray analyses of epithelial cell (IPEC-J2 cells).Our results provide evidence that already low basolateral concentrations of DON (50 ng/mL) influence the production of the BM protein laminin by epithelial cells.Thus, DON affects the composition of the BM.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anatomy, Otto-von-Guericke University Magdeburg, Leipziger Strasse 44, 39120, Magdeburg, Germany, constanze.nossol@med.ovgu.de.

ABSTRACT
The numerous pores in the basement membrane (BM) of the intestinal villi are essential for the communication of enterocytes with cells in the lamina propria, an important mechanism for the induction of intestinal immune responses. The intestinal epithelial barrier is affected by the mycotoxin deoxynivalenol (DON) from both the apical (luminal) and basolateral (serosal) side. The pig is the most susceptible species to the anorectic and immune-modulating effects of DON, which is most prevalent in crops. We analysed in pigs the effect of DON-contaminated feed on the composition and perforation of the BM and the presence of CD16(+) cells or their dendrites in the epithelium. In addition to in vivo experiments, in vitro studies were carried out. Using microarray analyses, the effects of DON on IPEC-J2 cells were studied with the focus on the BM. Our in vivo results showed in the control pigs: (1) a significant increased pore number (p ≤ 0.001) in the jejunum in comparison to ileum, (2) no difference in the pore size, and (3) comparable frequency of intraepithelial CD16(+) cells/dendrites in the jejunum and ileum. There was a marked trend that DON feeding increases: (1) the pore number in jejunum, and (2) the number of CD16(+) cells/dendrites in the epithelium (Tukey-Kramer; p = 0.055 and p = 0.067, respectively). The in vivo results were extended with microarray analyses of epithelial cell (IPEC-J2 cells). The down-regulation of genes like syndecan, fibulin 6 and BM-40 was observed. These proteins are important factors in the BM composition and in formation of pores. Our results provide evidence that already low basolateral concentrations of DON (50 ng/mL) influence the production of the BM protein laminin by epithelial cells. Thus, DON affects the composition of the BM.

Show MeSH
Related in: MedlinePlus