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DNA barcoding survey of Trichoderma diversity in soil and litter of the Colombian lowland Amazonian rainforest reveals Trichoderma strigosellum sp. nov. and other species.

López-Quintero CA, Atanasova L, Franco-Molano AE, Gams W, Komon-Zelazowska M, Theelen B, Müller WH, Boekhout T, Druzhinina I - Antonie Van Leeuwenhoek (2013)

Bottom Line: DNA barcoding of 107 strains based on the internal transcribed spacers 1 and 2 (ITS1 and 2) of the ribosomal RNA gene cluster and the partial sequence of the translation elongation factor 1 alpha (tef1) gene revealed that the diversity of Trichoderma was dominated (71 %) by three common cosmopolitan species, namely Trichoderma harzianum sensu lato (41 %), Trichoderma spirale (17 %) and Trichoderma koningiopsis (13 %).Multigene phylogenetic analysis and phenotype profiling of four strains with an ITS1 and 2 phylotype similar to Trichoderma strigosum revealed a new sister species of the latter that is described here as Trichoderma strigosellum sp. nov.Sequence similarity searches revealed that this species also occurs in soils of Malaysia and Cameroon, suggesting a pantropical distribution.

View Article: PubMed Central - PubMed

Affiliation: CBS Fungal Biodiversity Centre (CBS-KNAW), Utrecht, The Netherlands.

ABSTRACT
The diversity of Trichoderma (Hypocreales, Ascomycota) colonizing leaf litter as well as the rhizosphere of Garcinia macrophylla (Clusiaceae) was investigated in primary and secondary rain forests in Colombian Amazonia. DNA barcoding of 107 strains based on the internal transcribed spacers 1 and 2 (ITS1 and 2) of the ribosomal RNA gene cluster and the partial sequence of the translation elongation factor 1 alpha (tef1) gene revealed that the diversity of Trichoderma was dominated (71 %) by three common cosmopolitan species, namely Trichoderma harzianum sensu lato (41 %), Trichoderma spirale (17 %) and Trichoderma koningiopsis (13 %). Four ITS 1 and 2 phylotypes (13 strains) could not be identified with certainty. Multigene phylogenetic analysis and phenotype profiling of four strains with an ITS1 and 2 phylotype similar to Trichoderma strigosum revealed a new sister species of the latter that is described here as Trichoderma strigosellum sp. nov. Sequence similarity searches revealed that this species also occurs in soils of Malaysia and Cameroon, suggesting a pantropical distribution.

Show MeSH
Morphology of Trichoderma strigosellum sp. nov. CBS 102817. a. Colony on cornmeal agar (CMA) at room temperature; b. Branching conidiophores and phialides on CMA; c. Conidia on CMA; d. Drawing of conidation and conidia from CMA; e. Low magnification of SEM image of spore clusters; f. SEM of hyphae, phialides and conidiogenesis
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Fig6: Morphology of Trichoderma strigosellum sp. nov. CBS 102817. a. Colony on cornmeal agar (CMA) at room temperature; b. Branching conidiophores and phialides on CMA; c. Conidia on CMA; d. Drawing of conidation and conidia from CMA; e. Low magnification of SEM image of spore clusters; f. SEM of hyphae, phialides and conidiogenesis

Mentions: A new species is similar to T. koningii and T. koningiopsis but differentiated morphologically by much less developed aerial mycelium. Differing from its closest relative, T. strigosum, by complete absence of sterile conidiophore elongations and better growth at higher temperatures. Colonies on OA dark grey-green (Fig. 6) reaching 7–8 cm diameter after 5 days on CMA at 24 °C, and 9 cm after 6 days on CMD, SNA and OA at 27 °C, but only 0.1–0.4 cm on these three media at 36 °C. Submerged mycelium of young colonies irregularly and loosely branched, spreading radially; aerial mycelium with central conidiation after 4 days on CMD; zonate, with zones on OA approximately 15–18 mm distant, with scattered small pustules with deep green colour (27E8); growth on SNA sparse with loosely branched hyphae that form conidial heads, pustules hardly distinguishable, but after 7 days near the margin of the plate becoming distinct. Odor somewhat musty, but strain CBS 102805 had a coconut odor on OA.


DNA barcoding survey of Trichoderma diversity in soil and litter of the Colombian lowland Amazonian rainforest reveals Trichoderma strigosellum sp. nov. and other species.

López-Quintero CA, Atanasova L, Franco-Molano AE, Gams W, Komon-Zelazowska M, Theelen B, Müller WH, Boekhout T, Druzhinina I - Antonie Van Leeuwenhoek (2013)

Morphology of Trichoderma strigosellum sp. nov. CBS 102817. a. Colony on cornmeal agar (CMA) at room temperature; b. Branching conidiophores and phialides on CMA; c. Conidia on CMA; d. Drawing of conidation and conidia from CMA; e. Low magnification of SEM image of spore clusters; f. SEM of hyphae, phialides and conidiogenesis
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3824238&req=5

Fig6: Morphology of Trichoderma strigosellum sp. nov. CBS 102817. a. Colony on cornmeal agar (CMA) at room temperature; b. Branching conidiophores and phialides on CMA; c. Conidia on CMA; d. Drawing of conidation and conidia from CMA; e. Low magnification of SEM image of spore clusters; f. SEM of hyphae, phialides and conidiogenesis
Mentions: A new species is similar to T. koningii and T. koningiopsis but differentiated morphologically by much less developed aerial mycelium. Differing from its closest relative, T. strigosum, by complete absence of sterile conidiophore elongations and better growth at higher temperatures. Colonies on OA dark grey-green (Fig. 6) reaching 7–8 cm diameter after 5 days on CMA at 24 °C, and 9 cm after 6 days on CMD, SNA and OA at 27 °C, but only 0.1–0.4 cm on these three media at 36 °C. Submerged mycelium of young colonies irregularly and loosely branched, spreading radially; aerial mycelium with central conidiation after 4 days on CMD; zonate, with zones on OA approximately 15–18 mm distant, with scattered small pustules with deep green colour (27E8); growth on SNA sparse with loosely branched hyphae that form conidial heads, pustules hardly distinguishable, but after 7 days near the margin of the plate becoming distinct. Odor somewhat musty, but strain CBS 102805 had a coconut odor on OA.

Bottom Line: DNA barcoding of 107 strains based on the internal transcribed spacers 1 and 2 (ITS1 and 2) of the ribosomal RNA gene cluster and the partial sequence of the translation elongation factor 1 alpha (tef1) gene revealed that the diversity of Trichoderma was dominated (71 %) by three common cosmopolitan species, namely Trichoderma harzianum sensu lato (41 %), Trichoderma spirale (17 %) and Trichoderma koningiopsis (13 %).Multigene phylogenetic analysis and phenotype profiling of four strains with an ITS1 and 2 phylotype similar to Trichoderma strigosum revealed a new sister species of the latter that is described here as Trichoderma strigosellum sp. nov.Sequence similarity searches revealed that this species also occurs in soils of Malaysia and Cameroon, suggesting a pantropical distribution.

View Article: PubMed Central - PubMed

Affiliation: CBS Fungal Biodiversity Centre (CBS-KNAW), Utrecht, The Netherlands.

ABSTRACT
The diversity of Trichoderma (Hypocreales, Ascomycota) colonizing leaf litter as well as the rhizosphere of Garcinia macrophylla (Clusiaceae) was investigated in primary and secondary rain forests in Colombian Amazonia. DNA barcoding of 107 strains based on the internal transcribed spacers 1 and 2 (ITS1 and 2) of the ribosomal RNA gene cluster and the partial sequence of the translation elongation factor 1 alpha (tef1) gene revealed that the diversity of Trichoderma was dominated (71 %) by three common cosmopolitan species, namely Trichoderma harzianum sensu lato (41 %), Trichoderma spirale (17 %) and Trichoderma koningiopsis (13 %). Four ITS 1 and 2 phylotypes (13 strains) could not be identified with certainty. Multigene phylogenetic analysis and phenotype profiling of four strains with an ITS1 and 2 phylotype similar to Trichoderma strigosum revealed a new sister species of the latter that is described here as Trichoderma strigosellum sp. nov. Sequence similarity searches revealed that this species also occurs in soils of Malaysia and Cameroon, suggesting a pantropical distribution.

Show MeSH