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The significance of peroxisome function in chronological aging of Saccharomyces cerevisiae.

Lefevre SD, van Roermund CW, Wanders RJ, Veenhuis M, van der Klei IJ - Aging Cell (2013)

Bottom Line: We show that intact peroxisomes are an important factor in yeast chronological aging because all pex mutants showed a reduced chronological lifespan.The strongest reduction was observed in Δpex5 cells.Our data indicate that this is related to the complete inactivation of the peroxisomal β-oxidation pathway in these cells due to the mislocalization of thiolase.

View Article: PubMed Central - PubMed

Affiliation: Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, P.O. Box 11103, 9700CC, Groningen, The Netherlands.

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The significance of β-oxidation in chronological aging. (A) Chronological lifespan of Δpot1 and Δtgl3 cells relative to WT control cells. Cells were grown on 0.5% glucose. The data represent mean ± SEM from at least two experiments. (B) The mean chronological lifespans calculated from the data presented in panels A and D. *P < 0.05; **P < 0.01; ***P < 0.005. (C) Accumulation of free fatty acids (FFA) in WT, Δpex3, Δ3'pex5, and Δpot1 cells at day 3 of a chronological aging experiment. *P < 0.05. (D) Chronological lifespans of WT cells grown on 0.5% glucose or 0.1% oleic acid. Data represent mean ± SEM from at least three experiments. (E) Transmission electron microscopy images showing the high content of lipid bodies in WT cells grown on oleic acid during chronological aging.
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fig04: The significance of β-oxidation in chronological aging. (A) Chronological lifespan of Δpot1 and Δtgl3 cells relative to WT control cells. Cells were grown on 0.5% glucose. The data represent mean ± SEM from at least two experiments. (B) The mean chronological lifespans calculated from the data presented in panels A and D. *P < 0.05; **P < 0.01; ***P < 0.005. (C) Accumulation of free fatty acids (FFA) in WT, Δpex3, Δ3'pex5, and Δpot1 cells at day 3 of a chronological aging experiment. *P < 0.05. (D) Chronological lifespans of WT cells grown on 0.5% glucose or 0.1% oleic acid. Data represent mean ± SEM from at least three experiments. (E) Transmission electron microscopy images showing the high content of lipid bodies in WT cells grown on oleic acid during chronological aging.

Mentions: First, we deleted the third enzyme of the β-oxidation pathway (3-ketoacyl-CoA thiolase, Δpot1), which completely prohibits β-oxidation (see below; Fig. 5A,B). The mean and the maximal lifespan of Δpot1 cells are shortened (11.1 ± 1.44 days/20.4 ± 3.42 days) relative to WT controls (16 ± 2.52 days/26.6 ± 3.33 days; Fig. 4A,B, Table 1), indicating that β-oxidation is important for chronological aging.


The significance of peroxisome function in chronological aging of Saccharomyces cerevisiae.

Lefevre SD, van Roermund CW, Wanders RJ, Veenhuis M, van der Klei IJ - Aging Cell (2013)

The significance of β-oxidation in chronological aging. (A) Chronological lifespan of Δpot1 and Δtgl3 cells relative to WT control cells. Cells were grown on 0.5% glucose. The data represent mean ± SEM from at least two experiments. (B) The mean chronological lifespans calculated from the data presented in panels A and D. *P < 0.05; **P < 0.01; ***P < 0.005. (C) Accumulation of free fatty acids (FFA) in WT, Δpex3, Δ3'pex5, and Δpot1 cells at day 3 of a chronological aging experiment. *P < 0.05. (D) Chronological lifespans of WT cells grown on 0.5% glucose or 0.1% oleic acid. Data represent mean ± SEM from at least three experiments. (E) Transmission electron microscopy images showing the high content of lipid bodies in WT cells grown on oleic acid during chronological aging.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3824234&req=5

fig04: The significance of β-oxidation in chronological aging. (A) Chronological lifespan of Δpot1 and Δtgl3 cells relative to WT control cells. Cells were grown on 0.5% glucose. The data represent mean ± SEM from at least two experiments. (B) The mean chronological lifespans calculated from the data presented in panels A and D. *P < 0.05; **P < 0.01; ***P < 0.005. (C) Accumulation of free fatty acids (FFA) in WT, Δpex3, Δ3'pex5, and Δpot1 cells at day 3 of a chronological aging experiment. *P < 0.05. (D) Chronological lifespans of WT cells grown on 0.5% glucose or 0.1% oleic acid. Data represent mean ± SEM from at least three experiments. (E) Transmission electron microscopy images showing the high content of lipid bodies in WT cells grown on oleic acid during chronological aging.
Mentions: First, we deleted the third enzyme of the β-oxidation pathway (3-ketoacyl-CoA thiolase, Δpot1), which completely prohibits β-oxidation (see below; Fig. 5A,B). The mean and the maximal lifespan of Δpot1 cells are shortened (11.1 ± 1.44 days/20.4 ± 3.42 days) relative to WT controls (16 ± 2.52 days/26.6 ± 3.33 days; Fig. 4A,B, Table 1), indicating that β-oxidation is important for chronological aging.

Bottom Line: We show that intact peroxisomes are an important factor in yeast chronological aging because all pex mutants showed a reduced chronological lifespan.The strongest reduction was observed in Δpex5 cells.Our data indicate that this is related to the complete inactivation of the peroxisomal β-oxidation pathway in these cells due to the mislocalization of thiolase.

View Article: PubMed Central - PubMed

Affiliation: Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, P.O. Box 11103, 9700CC, Groningen, The Netherlands.

Show MeSH
Related in: MedlinePlus