The significance of peroxisome function in chronological aging of Saccharomyces cerevisiae.
Bottom Line: We show that intact peroxisomes are an important factor in yeast chronological aging because all pex mutants showed a reduced chronological lifespan.The strongest reduction was observed in Δpex5 cells.Our data indicate that this is related to the complete inactivation of the peroxisomal β-oxidation pathway in these cells due to the mislocalization of thiolase.
Affiliation: Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, P.O. Box 11103, 9700CC, Groningen, The Netherlands.Show MeSH
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Mentions: We first analyzed the CLS of a Δpex3 strain as this mutant shows the most severe peroxisome biogenesis defect. We did not observe significant differences in CLS (Fig. 1A,B, Table 1), upon growth of Δpex3 and wild-type (WT) control cultures on mineral media containing 2% glucose. At these conditions, the CLS of S. cerevisiae is mainly determined by acetic acid toxicity (Burtner et al., 2009). However, when Δpex3 cells were grown on mineral media containing 0.5% glucose, both the mean and maximal lifespan of the Δpex3 cultures were reduced (10.5 ± 2.98 days/22.9 ± 2.43 days) relative to the WT control (16 ± 2.52 days/26.6 ± 3.33 days) (Fig. 1C,D, Table 1). We therefore performed all subsequent CLS experiments with media containing 0.5% glucose.
Affiliation: Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, P.O. Box 11103, 9700CC, Groningen, The Netherlands.