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The significance of peroxisome function in chronological aging of Saccharomyces cerevisiae.

Lefevre SD, van Roermund CW, Wanders RJ, Veenhuis M, van der Klei IJ - Aging Cell (2013)

Bottom Line: We show that intact peroxisomes are an important factor in yeast chronological aging because all pex mutants showed a reduced chronological lifespan.The strongest reduction was observed in Δpex5 cells.Our data indicate that this is related to the complete inactivation of the peroxisomal β-oxidation pathway in these cells due to the mislocalization of thiolase.

View Article: PubMed Central - PubMed

Affiliation: Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, P.O. Box 11103, 9700CC, Groningen, The Netherlands.

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Peroxisome-deficient cells have a shorter lifespan than wild-type cells. (A) Chronological lifespan of WT and Δpex3 cells grown on 2% glucose. (B) The mean chronological lifespans of WT and Δpex3 cells calculated from the data in panel A. (C) Chronological lifespan of WT, Δpex3, Δpex5, and Δpex6 cells grown on 0.5% glucose. (D) The mean chronological lifespans of WT, Δpex3, Δpex5, and Δpex6 cells calculated from the data in panel C. Data represent mean ± SEM from at least three independent experiments. *P < 0.05; ***P < 0.005.
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fig01: Peroxisome-deficient cells have a shorter lifespan than wild-type cells. (A) Chronological lifespan of WT and Δpex3 cells grown on 2% glucose. (B) The mean chronological lifespans of WT and Δpex3 cells calculated from the data in panel A. (C) Chronological lifespan of WT, Δpex3, Δpex5, and Δpex6 cells grown on 0.5% glucose. (D) The mean chronological lifespans of WT, Δpex3, Δpex5, and Δpex6 cells calculated from the data in panel C. Data represent mean ± SEM from at least three independent experiments. *P < 0.05; ***P < 0.005.

Mentions: We first analyzed the CLS of a Δpex3 strain as this mutant shows the most severe peroxisome biogenesis defect. We did not observe significant differences in CLS (Fig. 1A,B, Table 1), upon growth of Δpex3 and wild-type (WT) control cultures on mineral media containing 2% glucose. At these conditions, the CLS of S. cerevisiae is mainly determined by acetic acid toxicity (Burtner et al., 2009). However, when Δpex3 cells were grown on mineral media containing 0.5% glucose, both the mean and maximal lifespan of the Δpex3 cultures were reduced (10.5 ± 2.98 days/22.9 ± 2.43 days) relative to the WT control (16 ± 2.52 days/26.6 ± 3.33 days) (Fig. 1C,D, Table 1). We therefore performed all subsequent CLS experiments with media containing 0.5% glucose.


The significance of peroxisome function in chronological aging of Saccharomyces cerevisiae.

Lefevre SD, van Roermund CW, Wanders RJ, Veenhuis M, van der Klei IJ - Aging Cell (2013)

Peroxisome-deficient cells have a shorter lifespan than wild-type cells. (A) Chronological lifespan of WT and Δpex3 cells grown on 2% glucose. (B) The mean chronological lifespans of WT and Δpex3 cells calculated from the data in panel A. (C) Chronological lifespan of WT, Δpex3, Δpex5, and Δpex6 cells grown on 0.5% glucose. (D) The mean chronological lifespans of WT, Δpex3, Δpex5, and Δpex6 cells calculated from the data in panel C. Data represent mean ± SEM from at least three independent experiments. *P < 0.05; ***P < 0.005.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3824234&req=5

fig01: Peroxisome-deficient cells have a shorter lifespan than wild-type cells. (A) Chronological lifespan of WT and Δpex3 cells grown on 2% glucose. (B) The mean chronological lifespans of WT and Δpex3 cells calculated from the data in panel A. (C) Chronological lifespan of WT, Δpex3, Δpex5, and Δpex6 cells grown on 0.5% glucose. (D) The mean chronological lifespans of WT, Δpex3, Δpex5, and Δpex6 cells calculated from the data in panel C. Data represent mean ± SEM from at least three independent experiments. *P < 0.05; ***P < 0.005.
Mentions: We first analyzed the CLS of a Δpex3 strain as this mutant shows the most severe peroxisome biogenesis defect. We did not observe significant differences in CLS (Fig. 1A,B, Table 1), upon growth of Δpex3 and wild-type (WT) control cultures on mineral media containing 2% glucose. At these conditions, the CLS of S. cerevisiae is mainly determined by acetic acid toxicity (Burtner et al., 2009). However, when Δpex3 cells were grown on mineral media containing 0.5% glucose, both the mean and maximal lifespan of the Δpex3 cultures were reduced (10.5 ± 2.98 days/22.9 ± 2.43 days) relative to the WT control (16 ± 2.52 days/26.6 ± 3.33 days) (Fig. 1C,D, Table 1). We therefore performed all subsequent CLS experiments with media containing 0.5% glucose.

Bottom Line: We show that intact peroxisomes are an important factor in yeast chronological aging because all pex mutants showed a reduced chronological lifespan.The strongest reduction was observed in Δpex5 cells.Our data indicate that this is related to the complete inactivation of the peroxisomal β-oxidation pathway in these cells due to the mislocalization of thiolase.

View Article: PubMed Central - PubMed

Affiliation: Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, P.O. Box 11103, 9700CC, Groningen, The Netherlands.

Show MeSH
Related in: MedlinePlus