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Occurrence of gustducin-immunoreactive cells in von Ebner's glands of guinea pigs.

Ibira Y, Yokosuka H, Haga-Tsujimura M, Yoshie S - Histochem. Cell Biol. (2013)

Bottom Line: Double immunofluorescence microscopy confirmed these cells to be entirely immunopositive to type III inositol 1,4,5-triphosphate receptor (IP3R-3), phospholipase Cβ2 (PLCβ2), and villin and also partly immunopositive to neuron-specific enolase (NSE) and calbindin D-28K.The gustducin-immunoreactive cells in the vallate taste buds exhibited completely the same immunoreactivities for these five molecules.Accordingly, the present results give credence to a consideration that the gustducin-immunnoreactive cells in both locations are identical in function(s) e.g., chemo-reception.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan.

ABSTRACT
An immunohistochemical examination of guinea-pig taste buds in vallate papillae revealed gustducin-immunoreactive cells in the area of von Ebner's glands, minor salivary glands. Since there have been no reports describing those cells in these locations for other species, we investigated these glands in order both to localize the cells and compare their immunoreactive characteristics with corresponding cells in the vallate taste buds. The gustducin-immunoreactive cells coincided with cells containing no secretory granules in the end portion of the glands, which was supported by the electron-microscopic immunocytochemistry. Double immunofluorescence microscopy confirmed these cells to be entirely immunopositive to type III inositol 1,4,5-triphosphate receptor (IP3R-3), phospholipase Cβ2 (PLCβ2), and villin and also partly immunopositive to neuron-specific enolase (NSE) and calbindin D-28K. The gustducin-immunoreactive cells in the vallate taste buds exhibited completely the same immunoreactivities for these five molecules. Accordingly, the present results give credence to a consideration that the gustducin-immunnoreactive cells in both locations are identical in function(s) e.g., chemo-reception.

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a–o Laser-scanning microscopic immunohistochemistry for gustducin (a–e) and the others (f–j); overlays of a–e and f–j (k–o) in von Ebner’s glands, respectively. The gustducin-immunoreactive cells are all immunopositive to IP3R-3 (k), PLCβ2 (l), and villin (m) but only partly immunopositive to NSE (n) and calbindin D-28K (o). Scale bars 10 μm
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Fig4: a–o Laser-scanning microscopic immunohistochemistry for gustducin (a–e) and the others (f–j); overlays of a–e and f–j (k–o) in von Ebner’s glands, respectively. The gustducin-immunoreactive cells are all immunopositive to IP3R-3 (k), PLCβ2 (l), and villin (m) but only partly immunopositive to NSE (n) and calbindin D-28K (o). Scale bars 10 μm

Mentions: The gustducin-immunoreactive cells in von Ebner’s glands were entirely immunopositive to IP3R-3, PLCβ2, and villin; they are partly immonopositive to NSE (69 % of the cells) and calbindin D-28K (72 %) (Fig. 4). Moreover, all the cells immunopositive to IP3R-3, PLCβ2, or villin were concomitantly immunoreactive for gustducin. These immunohistochemical data are summarized in Table 1.Fig. 4


Occurrence of gustducin-immunoreactive cells in von Ebner's glands of guinea pigs.

Ibira Y, Yokosuka H, Haga-Tsujimura M, Yoshie S - Histochem. Cell Biol. (2013)

a–o Laser-scanning microscopic immunohistochemistry for gustducin (a–e) and the others (f–j); overlays of a–e and f–j (k–o) in von Ebner’s glands, respectively. The gustducin-immunoreactive cells are all immunopositive to IP3R-3 (k), PLCβ2 (l), and villin (m) but only partly immunopositive to NSE (n) and calbindin D-28K (o). Scale bars 10 μm
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3824220&req=5

Fig4: a–o Laser-scanning microscopic immunohistochemistry for gustducin (a–e) and the others (f–j); overlays of a–e and f–j (k–o) in von Ebner’s glands, respectively. The gustducin-immunoreactive cells are all immunopositive to IP3R-3 (k), PLCβ2 (l), and villin (m) but only partly immunopositive to NSE (n) and calbindin D-28K (o). Scale bars 10 μm
Mentions: The gustducin-immunoreactive cells in von Ebner’s glands were entirely immunopositive to IP3R-3, PLCβ2, and villin; they are partly immonopositive to NSE (69 % of the cells) and calbindin D-28K (72 %) (Fig. 4). Moreover, all the cells immunopositive to IP3R-3, PLCβ2, or villin were concomitantly immunoreactive for gustducin. These immunohistochemical data are summarized in Table 1.Fig. 4

Bottom Line: Double immunofluorescence microscopy confirmed these cells to be entirely immunopositive to type III inositol 1,4,5-triphosphate receptor (IP3R-3), phospholipase Cβ2 (PLCβ2), and villin and also partly immunopositive to neuron-specific enolase (NSE) and calbindin D-28K.The gustducin-immunoreactive cells in the vallate taste buds exhibited completely the same immunoreactivities for these five molecules.Accordingly, the present results give credence to a consideration that the gustducin-immunnoreactive cells in both locations are identical in function(s) e.g., chemo-reception.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan.

ABSTRACT
An immunohistochemical examination of guinea-pig taste buds in vallate papillae revealed gustducin-immunoreactive cells in the area of von Ebner's glands, minor salivary glands. Since there have been no reports describing those cells in these locations for other species, we investigated these glands in order both to localize the cells and compare their immunoreactive characteristics with corresponding cells in the vallate taste buds. The gustducin-immunoreactive cells coincided with cells containing no secretory granules in the end portion of the glands, which was supported by the electron-microscopic immunocytochemistry. Double immunofluorescence microscopy confirmed these cells to be entirely immunopositive to type III inositol 1,4,5-triphosphate receptor (IP3R-3), phospholipase Cβ2 (PLCβ2), and villin and also partly immunopositive to neuron-specific enolase (NSE) and calbindin D-28K. The gustducin-immunoreactive cells in the vallate taste buds exhibited completely the same immunoreactivities for these five molecules. Accordingly, the present results give credence to a consideration that the gustducin-immunnoreactive cells in both locations are identical in function(s) e.g., chemo-reception.

Show MeSH