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Occurrence of gustducin-immunoreactive cells in von Ebner's glands of guinea pigs.

Ibira Y, Yokosuka H, Haga-Tsujimura M, Yoshie S - Histochem. Cell Biol. (2013)

Bottom Line: Double immunofluorescence microscopy confirmed these cells to be entirely immunopositive to type III inositol 1,4,5-triphosphate receptor (IP3R-3), phospholipase Cβ2 (PLCβ2), and villin and also partly immunopositive to neuron-specific enolase (NSE) and calbindin D-28K.The gustducin-immunoreactive cells in the vallate taste buds exhibited completely the same immunoreactivities for these five molecules.Accordingly, the present results give credence to a consideration that the gustducin-immunnoreactive cells in both locations are identical in function(s) e.g., chemo-reception.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan.

ABSTRACT
An immunohistochemical examination of guinea-pig taste buds in vallate papillae revealed gustducin-immunoreactive cells in the area of von Ebner's glands, minor salivary glands. Since there have been no reports describing those cells in these locations for other species, we investigated these glands in order both to localize the cells and compare their immunoreactive characteristics with corresponding cells in the vallate taste buds. The gustducin-immunoreactive cells coincided with cells containing no secretory granules in the end portion of the glands, which was supported by the electron-microscopic immunocytochemistry. Double immunofluorescence microscopy confirmed these cells to be entirely immunopositive to type III inositol 1,4,5-triphosphate receptor (IP3R-3), phospholipase Cβ2 (PLCβ2), and villin and also partly immunopositive to neuron-specific enolase (NSE) and calbindin D-28K. The gustducin-immunoreactive cells in the vallate taste buds exhibited completely the same immunoreactivities for these five molecules. Accordingly, the present results give credence to a consideration that the gustducin-immunnoreactive cells in both locations are identical in function(s) e.g., chemo-reception.

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a–c Semithin sections of von Ebner’s glands stained with toluidine blue (a, b) or immunostained for gustducin (c). a End portion of the glands. Predominant are the secretory cells accumulating secretory granules in the supranuclear cytoplasm (asterisks). A clear cell intermingles with the secretory cells (arrow). Note that the clear cell lacks secretory granules. b, c Consecutive sections. The clear cells exclusively exhibit the immunoreactivity for gustducin (arrowheads). Scale bars 10 μm
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Fig2: a–c Semithin sections of von Ebner’s glands stained with toluidine blue (a, b) or immunostained for gustducin (c). a End portion of the glands. Predominant are the secretory cells accumulating secretory granules in the supranuclear cytoplasm (asterisks). A clear cell intermingles with the secretory cells (arrow). Note that the clear cell lacks secretory granules. b, c Consecutive sections. The clear cells exclusively exhibit the immunoreactivity for gustducin (arrowheads). Scale bars 10 μm

Mentions: Observation of 0.5-μm semithin sections of von Ebner’s glands stained with toluidine blue readily confirmed that the end portion, tubulo-alveolar in shape, consisted of typical serous cells accumulating densely stained secretory granules in the supranuclear cytoplasm (Fig. 2a). Careful examinations often revealed cells with different aspects in the end portion. These cells appeared clear, being oval to triangular in profile, and contained no secretory granules (Fig. 2a, b). They usually existed solitarily in the end portion. All the clear cells exhibited immunoreactivity for gustducin (Fig. 2b, c). In support of these light-microscopic findings, the electron-microscopic immunocytochemistry showed that cells immunopositive to gustducin did not contain any secretory granules in the supranuclear cytoplasm (Fig. 3). Although we have not yet examined precisely the fine structure of the glands by conventional electron microscopy, the immunoreactive cell developed endoplasmic reticulum-like structures in the whole cytoplasm and contained a round, large, and electron-lucent nucleus (Fig. 3). These structural features resembled those of the Type II cell in the vallate taste bud of guinea pigs (Yoshie et al. 1990). However, we failed to detect the gustducin-immunoreactive cells forming microvilli at the apical surface.Fig. 2


Occurrence of gustducin-immunoreactive cells in von Ebner's glands of guinea pigs.

Ibira Y, Yokosuka H, Haga-Tsujimura M, Yoshie S - Histochem. Cell Biol. (2013)

a–c Semithin sections of von Ebner’s glands stained with toluidine blue (a, b) or immunostained for gustducin (c). a End portion of the glands. Predominant are the secretory cells accumulating secretory granules in the supranuclear cytoplasm (asterisks). A clear cell intermingles with the secretory cells (arrow). Note that the clear cell lacks secretory granules. b, c Consecutive sections. The clear cells exclusively exhibit the immunoreactivity for gustducin (arrowheads). Scale bars 10 μm
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3824220&req=5

Fig2: a–c Semithin sections of von Ebner’s glands stained with toluidine blue (a, b) or immunostained for gustducin (c). a End portion of the glands. Predominant are the secretory cells accumulating secretory granules in the supranuclear cytoplasm (asterisks). A clear cell intermingles with the secretory cells (arrow). Note that the clear cell lacks secretory granules. b, c Consecutive sections. The clear cells exclusively exhibit the immunoreactivity for gustducin (arrowheads). Scale bars 10 μm
Mentions: Observation of 0.5-μm semithin sections of von Ebner’s glands stained with toluidine blue readily confirmed that the end portion, tubulo-alveolar in shape, consisted of typical serous cells accumulating densely stained secretory granules in the supranuclear cytoplasm (Fig. 2a). Careful examinations often revealed cells with different aspects in the end portion. These cells appeared clear, being oval to triangular in profile, and contained no secretory granules (Fig. 2a, b). They usually existed solitarily in the end portion. All the clear cells exhibited immunoreactivity for gustducin (Fig. 2b, c). In support of these light-microscopic findings, the electron-microscopic immunocytochemistry showed that cells immunopositive to gustducin did not contain any secretory granules in the supranuclear cytoplasm (Fig. 3). Although we have not yet examined precisely the fine structure of the glands by conventional electron microscopy, the immunoreactive cell developed endoplasmic reticulum-like structures in the whole cytoplasm and contained a round, large, and electron-lucent nucleus (Fig. 3). These structural features resembled those of the Type II cell in the vallate taste bud of guinea pigs (Yoshie et al. 1990). However, we failed to detect the gustducin-immunoreactive cells forming microvilli at the apical surface.Fig. 2

Bottom Line: Double immunofluorescence microscopy confirmed these cells to be entirely immunopositive to type III inositol 1,4,5-triphosphate receptor (IP3R-3), phospholipase Cβ2 (PLCβ2), and villin and also partly immunopositive to neuron-specific enolase (NSE) and calbindin D-28K.The gustducin-immunoreactive cells in the vallate taste buds exhibited completely the same immunoreactivities for these five molecules.Accordingly, the present results give credence to a consideration that the gustducin-immunnoreactive cells in both locations are identical in function(s) e.g., chemo-reception.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan.

ABSTRACT
An immunohistochemical examination of guinea-pig taste buds in vallate papillae revealed gustducin-immunoreactive cells in the area of von Ebner's glands, minor salivary glands. Since there have been no reports describing those cells in these locations for other species, we investigated these glands in order both to localize the cells and compare their immunoreactive characteristics with corresponding cells in the vallate taste buds. The gustducin-immunoreactive cells coincided with cells containing no secretory granules in the end portion of the glands, which was supported by the electron-microscopic immunocytochemistry. Double immunofluorescence microscopy confirmed these cells to be entirely immunopositive to type III inositol 1,4,5-triphosphate receptor (IP3R-3), phospholipase Cβ2 (PLCβ2), and villin and also partly immunopositive to neuron-specific enolase (NSE) and calbindin D-28K. The gustducin-immunoreactive cells in the vallate taste buds exhibited completely the same immunoreactivities for these five molecules. Accordingly, the present results give credence to a consideration that the gustducin-immunnoreactive cells in both locations are identical in function(s) e.g., chemo-reception.

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